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Recombinant SIRT1 Antibody - Primary antibody, specific to SIRT1, Rabbit IgG

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Recombinant SIRT1 Antibody - Primary antibody, specific to SIRT1, Rabbit IgG

Grade & Purity:

See COA

Application:

IF/ICC
IHC
IP
WB

Host species: Rabbit
Reactive species: Human
Isotype: Rabbit IgG
Conjugation: Unconjugated

In stock

Item Number

Ab127656

Grouped product items
SKU	Size	Availability	Price	Qty
Ab127656-10μl	10μl	2	$59.90
Ab127656-50μl	50μl	1	$169.90
Ab127656-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$249.90
Ab127656-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,299.90

Recombinant; Rabbit anti Human SIRT1 Antibody; WB, IHC, IF/ICC, IP; Unconjugated

View related series

Click to view target information: SIRT1

Accession#:Q96EB6 (2) Gene ID:23411 (2) SIR2alpha (1) SIRT1 (2) Sirtuin 1 (1)

Basic Description

Product Name	Recombinant SIRT1 Antibody - Primary antibody, specific to SIRT1, Rabbit IgG
Synonyms	2.3.1.- \| EC 2.3.1.286 \| hSIR2 \| hSIRT1 \| NAD-dependent protein deacylase sirtuin-1 \| Regulatory protein SIR2 homolog 1 \| SIR2-like protein 1 \| EC 3.5.1 \| S. cerevisiae, homolog) 1 \| SIR2alpha \| SIR2L1 \| SIR2-like protein 1 \| SIRT1 \| Sirtuin 1 \| SIR2 \| 75
Specifications & Purity	Recombinant, ExactAb™, Validated, See COA
Host species	Rabbit
Specificity	SIRT1
Immunogen	A synthetic peptide derived from human SIRT1(AA 700-740)
Positive Control	WB: A549, HeLa, K562, MCF7, Jurkat and Hep G2 cell lysates. IHC: Rat stomach, Human pituitary tumor, Human colon, Human placenta, Mouse lung, Human kidney tissue. IF/ICC: HeLa cells. IP: HeLa lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human SIRT1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC, IP and so on.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

SIRT1 Tchem NAD-dependent protein deacetylase sirtuin-1 (0 Activities)

Discover Target: SIRT1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

SIRT1 Tchem NAD-dependent deacetylase sirtuin 1 (3505 Activities)

Discover Target: SIRT1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Western Blot: Recombinant SIRT1 Antibody (Ab127656)

Recombinant SIRT1 Antibody (Ab127656) - Western Blot
All lanes: Recombinant SIRT1 Antibody (Ab127656) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 82 kDa
Observed band size: 116 kDa
Exposure time: 155.2 s

IF/ICC: Recombinant SIRT1 Antibody (Ab127656)

Recombinant SIRT1 Antibody (Ab127656) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant SIRT1 Antibody (Ab127656) at 1/50 dilution.

IF/ICC: Recombinant SIRT1 Antibody (Ab127656)

Recombinant SIRT1 Antibody (Ab127656) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant SIRT1 Antibody (Ab127656).

IP: Recombinant SIRT1 Antibody (Ab127656)

Recombinant SIRT1 Antibody (Ab127656) - IP
Immunoprecipitate (IP) analysis using Recombinant SIRT1 Antibody (Ab127656) at 1/50 dilution. (WB with Recombinant SIRT1 Antibody (Ab127656) at 1/6000 dilution)

IHC: Recombinant SIRT1 Antibody (Ab127656)

Recombinant SIRT1 Antibody (Ab127656) - IHC
Immunohistochemical analysis of paraffin-embedded Rat stomach, using Recombinant SIRT1 Antibody (Ab127656) at 1/100 dilu

IHC: Recombinant SIRT1 Antibody (Ab127656)

Recombinant SIRT1 Antibody (Ab127656) - IHC
Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using Recombinant SIRT1 Antibody (Ab127656) at 1/100 dilution.

IHC: Recombinant SIRT1 Antibody (Ab127656)

Recombinant SIRT1 Antibody (Ab127656) - IHC
Immunohistochemical analysis of paraffin-embedded Human colon, using Recombinant SIRT1 Antibody (Ab127656) at 1/100 dilution.

IHC: Recombinant SIRT1 Antibody (Ab127656)

Recombinant SIRT1 Antibody (Ab127656) - IHC
Immunohistochemical analysis of paraffin-embedded Human placenta, using Recombinant SIRT1 Antibody (Ab127656) at 1/100 dilution.

IHC: Recombinant SIRT1 Antibody (Ab127656)

Recombinant SIRT1 Antibody (Ab127656) - IHC
Immunohistochemical analysis of paraffin-embedded Mouse lung, using Recombinant SIRT1 Antibody (Ab127656) at 1/100 dilution.

IHC: Recombinant SIRT1 Antibody (Ab127656)

Recombinant SIRT1 Antibody (Ab127656) - IHC
Immunohistochemical analysis of paraffin-embedded human kidney, using Recombinant SIRT1 Antibody (Ab127656).

Application

Application	Dilution info
WB	1/500-1/2000
IF/ICC	1/50 - 1/200
IHC	1/50-1/200
IP	1/30

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0708453	Certificate of Analysis	Jul 17, 2024	Ab127656
ZJ24F0708452	Certificate of Analysis	Jul 17, 2024	Ab127656

Documents & Articles

General Immunoprecipitation Protocol

Categories: Protocols

Tags:

Immunoprecipitation Western blot Cell lysate Co-immunoprecipitation IP Protein interaction Protein A/G magnetic beads

RNA Immunoprecipitation (RIP) Protocol

Categories: Protocols

Tags:

IP Protein A/G magnetic beads RNA immunoprecipitation RIP RNA protein interaction Isotype control antibody Input Crosslinking

Immobilized IP Protocol

Categories: Protocols

Tags:

Immunoprecipitation WB Western blot IP Protein interaction Immobilization immunoprecipitation Immobilized antibody

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