Grouped product items

SKU

Size

Availability

Price

Qty

Ab182004-10μl

10μl

Available within 8-12 weeks(?)

$69.90

Ab182004-50μl

50μl

$199.90

Ab182004-100μl

100μl

Available within 8-12 weeks(?)

$319.90

Ab182004-1ml

1ml

Available within 8-12 weeks(?)

$2,099.90

Basic Description

Product Name	Recombinant S100A8 Antibody - Primary antibody, specific to S100A8, Rabbit IgG
Synonyms	Calgranulin-A \| Calprotectin L1L subunit \| CFAG \| Cystic fibrosis antigen \| Leukocyte L1 complex light chain \| Migration inhibitory factor-related protein 8 \| MRP-8 \| p8 \| S100 calcium-binding protein A8 \| Urinary stone protein band A \| Calgranulin-A anti
Specifications & Purity	Recombinant, ExactAb™, Validated, See COA
Host species	Rabbit
Specificity	S100A8
Immunogen	A synthetic peptide derived from human S100A8 (AA 50-93).
Positive Control	WB: HL-60 lysates. IHC: Human breast cancer tissue.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human S100A8 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/2000 IHC: 1/50-1/200 Protein Function： S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

S100A8 Tbio Protein S100-A8 (0 Activities)

Discover Target: S100A8

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: Recombinant S100A8 Antibody (Ab182004)

Recombinant S100A8 Antibody (Ab182004) - Western Blot
All lanes: Recombinant S100A8 Antibody (Ab182004) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 11 kDa
Observed band size: 13 kDa
Exposure time: 29.9 s

IHC: Recombinant S100A8 Antibody (Ab182004)

Recombinant S100A8 Antibody (Ab182004) - IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Recombinant S100A8 Antibody (Ab182004).

Application

Application	Dilution info
WB	1/500-1/2000
IHC	1/50-1/200

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Documents & Articles

Protocol of Western Blotting For High Molecular Weights

Categories: Protocols

Tags:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

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Recombinant S100A8 Antibody - Primary antibody, specific to S100A8, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

SKU	Size	Availability	Price
Ab182004-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab182004-50μl	50μl	1	$199.90
Ab182004-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$319.90
Ab182004-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,099.90

Recombinant S100A8 Antibody - Primary antibody, specific to S100A8, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews