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蛋白酶K

47篇引用文献 COA COA
    级别和纯度:
  • EnzymoPure™
    •
  • 冻干粉，≥30 units/mg protein
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货号 (SKU) 包装规格 是否现货 价格 数量
P109033-25mg
 25mg 现货 Stock Image
P109033-100mg
 100mg 现货 Stock Image
P109033-500mg
 500mg 现货 Stock Image
P109033-1g
 1g 现货 Stock Image
P109033-5g
 5g 现货 Stock Image
P109033-25g
 25g 现货 Stock Image
P109033-100g
 100g 现货 Stock Image
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Amino Acid/Protein Metabolism (4559) Metabolic Enzyme/Protease (4889) Ser/Thr Protease (102) 水解酶 (20)
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基本描述

产品名称 蛋白酶K
别名 肽链内断酶K
英文别名 (4-nitrophenyl)methyl (4R,5S,6S)-6-[(1R)-1-hydroxyethyl]-4-methyl-7-oxo-3,4-diphenoxy-5-phosphonooxy-1-azabicyclo[3.2.0]hept-2-ene-2-carboxylate | BP178285 | AKOS032949645 | Endopeptidase K
规格或纯度 EnzymoPure™, 冻干粉，≥30 units/mg protein
生化机理 蛋白酶 K 是一种稳定的高活性丝氨酸蛋白酶。晶体和分子结构研究的证据表明，该酶属于枯草蛋白酶家族，具有活性位催化三元组（Asp39-His69-Ser224）。它在 pH 值、缓冲盐、洗涤剂（SDS）和温度等多种环境中都很稳定。在 0.1-0.5% SDS 存在的情况下，蛋白酶 K 仍能保持活性，并能消化 DNA 制剂中的各种蛋白质和核酸酶，而不会影响分离 DNA 的完整性。
产品介绍

蛋白酶K(Proteinase K)，属丝氨酸蛋白酶类，1974年，Wolfgang Ebeling等利用沉淀、离子交换层析和凝聚层析等方法在林伯氏白色念球菌的培养基中分离纯化出蛋白酶K。因能合成该种蛋白酶的微生物能在以角蛋白(Keratin)为唯一碳氮源的环境中生长，即能够消化角蛋白，因此被命名为蛋白酶K(Proteinase K)。蛋白酶K由一条肽链组成，包含277个氨基酸残基，相对分子质量为28.9kDa。与其它的蛋白酶相比较，蛋白酶K具有高活力和高稳定性，其酶活不会被尿素、SDS和EDTA等变性剂抑制，且在高温、高盐或较高的pH等条件下，蛋白酶K仍能保持较高活性。因该酶在较广的pH范围(4～12.5)内及高温(50～70℃)均有活性，在提取DNA和RNA的缓冲液中具有很高的活性，可用于质粒或基因组DNA、RNA的分离，是DNA提取的关键试剂。

近年来，随着研究的深入，蛋白酶K(Proteinase K)的应用不仅仅局限在医疗诊断及科研领域。在实现规模化工业生产的前提下，蛋白酶K还可以替代作用范围较窄的其它蛋白酶，应用于工业制革、食品加工、饲料生产、造纸等行业中。另外，蛋白酶K也可以替代传统的可能存在污染的化学方法处理原材料，去除不必要的蛋白质杂质。

阿拉丁推出的蛋白酶K(Proteinase K)，纯度高，比酶活高。该产品应用于新冠核酸提取试剂盒，能完全消化各种核酸酶和其他蛋白质，防止病毒核酸降解，优化提取效率，可满足COVID-19/HCV/HBV等检测需求。

本产品来源于表达经过定点突变优化的林伯氏白色念球菌（Engyodontium album）蛋白酶 K 基因的毕赤酵母


特点

1、According to the SDS-PAGE image,the purity of Proteinase K is more than 95% and the molecular weight is 28.9 kDa.

2、Detect DNase residue by agarose gel electrophores.

3、Detect Nucleic acid residue by agarose gel electrophores.

4、Detect RNase residue by agarose gel electrophores.

5、Using the absorbance A275 as the vertical axis and different concentrations of tyrosine as the horizontal axis, a standard curve was drawn, and the enzyme activity was calculated>30U/mg.

Product Introduce

Proteinase K, originally isolated from the mold Tritirachium album, is a serine protease with broad substrate specificity and relatively high proteolytic activity. It preferentially cleaves ester and peptide bonds adjacent to the C-termini of hydrophobic, aliphatic, or aromatic amino acids. aladdin's proteinase K is characterized by high purity, sterility, no bio-burden, and no presence of DNAse, RNAse, DNA, and RNA contaminants. It is a good partner in DNA and RNA extraction for you.

Features

1、According to the SDS-PAGE image,the purity of Proteinase K is more than 95% and the molecular weight is 28.9 kDa.

2、Detect DNase residue by agarose gel electrophores.

3、Detect Nucleic acid residue by agarose gel electrophores.

4、Detect RNase residue by agarose gel electrophores.

5、Using the absorbance A275 as the vertical axis and different concentrations of tyrosine as the horizontal axis, a standard curve was drawn, and the enzyme activity was calculated>30U/mg.

储存与运输

浓度 冻干粉，≥30 units/mg protein
储存温度 -20°C储存
运输条件 超低温冰袋运输
CAS编号和信息 39450-01-6
单位定义 37℃ pH7.5 条件下，每分钟可水解酪蛋白底物生成1 μmol 酪氨酸的蛋白酶K的量定义为一个单位（U）。
分子类型 蛋白质

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批号(Lot Number) 证书类型 货号
A2419061 分析证书 P109033
C2211248 分析证书 P109033
J2330021 分析证书 P109033
J2311075 分析证书 P109033
E2520626 分析证书 P109033
E2520627 分析证书 P109033
E2520628 分析证书 P109033
E2520629 分析证书 P109033
E2520630 分析证书 P109033
E2520631 分析证书 P109033
A2521363 分析证书 P109033
A2521370 分析证书 P109033
A2521412 分析证书 P109033
A2521359 分析证书 P109033
A2521365 分析证书 P109033
H2501076 分析证书 P109033
G2412542 分析证书 P109033
G2412544 分析证书 P109033
G2412554 分析证书 P109033
G2412543 分析证书 P109033
I2419068 分析证书 P109033
I2423642 分析证书 P109033
G2412539 分析证书 P109033
G2412540 分析证书 P109033
G2412541 分析证书 P109033
H2429117 分析证书 P109033
C2406011 分析证书 P109033
C2406012 分析证书 P109033
K2125251 分析证书 P109033
K2125264 分析证书 P109033
K2125249 分析证书 P109033
C2110015 分析证书 P109033
C2110016 分析证书 P109033
K2217188 分析证书 P109033
I2222451 分析证书 P109033
C2303238 分析证书 P109033
K2024181 分析证书 P109033
C2211247 分析证书 P109033
C2211179 分析证书 P109033
A2303338 分析证书 P109033
A2316087 分析证书 P109033
G2314185 分析证书 P109033
L2305179 分析证书 P109033
D2423042 分析证书 P109033
G2404076 分析证书 P109033

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