按主题分类的文章 "Nuclease"

1. Dual Cross-linking ChIP Protocol
   发布于 2025年3月28日

   26
   ChIP is a powerful tool that uses isolated chromatin and antibodies against the target antigen to determine whether the target binds to a specific DNA sequence or to map its distribution across the genome (ChIP-seq).
2. Apoptosis DNA Fragmentation Analysis Protocol
   发布于 2025年3月27日

   0
   The DNA of apoptotic cells is cut into fragments of about 200 base pairs under the action of caspase-activated nuclease (CAD), forming DNA ladders. By detecting DNA fragmentation during apoptosis, the degree of apoptosis can be evaluated.
3. CUT&RUN-seq protocol
   发布于 2024年12月23日

   0
   Targets and Release Using Nuclease (CUT&RUN) experimental technology is an emerging chromatin analysis method for studying protein-DNA interactions. It uses antibodies to specifically label target proteins, and then uses nucleases bound to antibodies (such as pAG-MNase) to cut DNA fragments that ...
4. CRISPR/Cas9 and targeted genome editing
   发布于 2022年11月21日

   10
   It is a long-term goal for biomedical researchers to develop efficient and reliable methods to make accurate and targeted changes in the genome of living cells. In recent years, a new tool based on bacterial CRISPR-associated protein-9 nuclease (Cas9) from Streptococcus pyogenes has aroused ...