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N-Succinyl-Gly-Gly-Phe-p-nitroanilideCAS号: 68982-90-1 Compound CID : 40520303分子式: C23H25N5O8 分子量: 499.47IUPAC Name: 4-[[2-[[2-[[(2S)-1-(4-nitroanilino)-1-oxo-3-phenylpropan-2-yl]amino]-2-oxoethyl]amino]-2-oxoethyl]amino]-4-oxobutanoic acidSMILES: C1=CC=C(C=C1)CC(C(=O)NC2=CC=C(C=C2)[N+](=O)[O-])NC(=O)CNC(=O)CNC(=O)CCC(=O)OInChIKey: CFYIUBWVKZQDOG-SFHVURJKSA-NInChI: 1S/C23H25N5O8/c29-19(10-11-22(32)33)24-13-20(30)25-14-21(31)27-18(12-15-4-2-1-3-5-15)23(34)26-16-6-8-17(9-7-16)28(35)36/h1-9,18H,10-14H2,(H,24,29)(H,25,30)(H,26,34)(H,27,31)(H,32,33)/t18-/m0/s1
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Recombinant EN-TEV Protease Protein种属: 烟草蚀纹病毒(Tobacco etch virus(TEV)) Accession #: P04517表达系统: E. coli 蛋白标签: N-His 内毒素水平: <1.0 EU/μg生物活性: Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser , with the cleavage point after Gln. One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.Complete digestion of 50µg of fusion KGF-MBP tag protein using 1µg TEV enzyme (in 1/50 ratio) in either 1 hours at 30 °C or overnight at 4 °C. The "standard" reaction buffer for TEV protease is 50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA and 1mM DTT.
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Recombinant Human MMP9 Protein种属: 人(Human) Accession #: P14780表达系统: HEK293 蛋白标签: C-His 内毒素水平: <0.1 EU/μg生物活性: Measured in a cell migration assay using A549 cells. 1 ng/mL of Recombinant Human MMP-9 can effectively induce A549 cells migration. Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is >1100 pmoles/min/μg.
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Recombinant Human CD10 Protein种属: 人(Human) Accession #: P08473表达系统: HEK293 蛋白标签: C-His 内毒素水平: <0.1 EU/μg生物活性: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp)-OH, (R&D Systems, Catalog # ES005). The specific activity is >1,500 pmoles/min/μg.
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Recombinant Human Cathepsin S protein种属: 人(Human) Accession #: P25774-1表达系统: HEK293 蛋白标签: C-8×His 内毒素水平: <0.1 EU/μg生物活性: Measured by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl) acetyl-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys (2, 4-dinitrophenyl)-NH2. Cleavage of substrate can be measured using excitation and emission wavelength at 320 nm and 405 nm, respectively.The specific activity is >300 pmoles/min/μg.
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Recombinant Mouse MMP-9 Protein种属: 小鼠(Mouse) Accession #: P41245表达系统: HEK293 蛋白标签: C-His 内毒素水平: <0.1 EU/μg生物活性: Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is >1900 pmol/min/μg.
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T4 DNA 聚合酶种属: Escherichia phage T4 Accession #: P04415表达系统: E. coli生物活性: Catalytic incorporation of 10nmol deoxyribonucleotides (dNTPs) into polynucleotides at 37 ℃ for 30 minutes. The required enzyme quantity is defined as one active unit.
