按主题分类的文章 "protease"

1. Ubiquitin–Proteasome System (UPS)
   发布于 2025年9月5日

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   The UPS is the primary pathway for protein degradation within cells. It tags specific substrate proteins with ubiquitin (Ub), which are then recognized by the 26S proteasome and degraded into small peptides. This system maintains protein homeostasis and regulates processes such as the cell ...
2. Recombinant Trypsin
   发布于 2025年9月1日

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   Trypsin is a typical endopeptidase belonging to the serine protease family. Its primary catalytic mechanism involves the specific hydrolysis of peptide bonds at the carboxyl side of basic amino acids (arginine or lysine).
3. In Situ Hybridization (ISH) Protocol
   发布于 2024年12月17日

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   In situ hybridization (ISH) is an experimental technique used to detect and locate specific nucleic acid sequences (DNA or RNA) in cells or tissues. It uses labeled nucleic acid probes to specifically bind to target nucleic acid sequences, thereby observing and analyzing the expression and ...
4. Editing sugar chains on therapeutic proteins with glycosidase
   发布于 2022年11月4日

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   One of the most common post-translational modifications of eukaryotic proteins is glycosylation. Glycosylation of proteins can affect many biological activities. For therapeutic glycoproteins, it can alter biological activity, targeting, transport, serum half-life, clearance and receptor ...