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Recombinant TCR γ/δ Antibody (FITC)

Recombinant TCR γ/δ Antibody (FITC)

COA

级别和纯度:

已验证

ExactAb™

5 μL/test

应用:

Flow Cytometry

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: P22D01
偶联: FITC

有货

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货号 (SKU)	包装规格	是否现货
Ab216133-10T	10T	现货
Ab216133-25T	25T	现货
Ab216133-100T	100T	现货

查看相关系列

点击查看靶蛋白信息: TRDC 点击查看靶蛋白信息: TRGC2

Accession#: B7Z8K6 (4) Accession#: P03986 (4) FITC (209) Gene ID: 6964 (4) Gene ID: 6965 (4) TRDC (3) TRGC2 (3)

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基本信息

产品名称	Recombinant TCR γ/δ Antibody (FITC)
规格或纯度	ExactAb™, 已验证, 5 μL/test
生化机理	Gamma-delta TRs recognize a variety of self and foreign non-peptide antigens frequently expressed at the epithelial boundaries between the host and external environment, including endogenous lipids presented by MH-like protein CD1D and phosphoantigens presented by butyrophilin-like molecule BTN3A1. Upon antigen recognition induces rapid, innate-like immune responses involved in pathogen clearance and tissue repair. Binding of gamma-delta TR complex to antigen triggers phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) in the CD3 chains by the LCK and FYN kinases, allowing the recruitment, phosphorylation, and activation of ZAP70 that facilitates phosphorylation of the scaffolding proteins LCP2 and LAT. This lead to the formation of a supramolecular signalosome that recruits the phospholipase PLCG1, resulting in calcium mobilization and ERK activation, ultimately leading to T cell expansion and differentiation into effector cells. Gamma-delta TRs are produced through somatic rearrangement of a limited repertoire of variable (V), diversity (D), and joining (J) genes. The potential diversity of gamma-delta TRs is conferred by the unique ability to rearrange (D) genes in tandem and to utilize all three reading frames. The combinatorial diversity is considerably increased by the sequence exonuclease trimming and random nucleotide (N) region additions which occur during the V-(D)-J rearrangements.
宿主种属	小鼠(Mouse)
特异性	Human TCR γ/δ
种属反应性	人(Human)
阳性对照	Flow Cytometry: Human peripheral blood lymphocytes
偶联	FITC
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	517nm
Ex/Em(nm)	Ex:498nm;Em:517nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	5 μL/test
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

TRDC Tdark T 细胞受体δ常数(T cell receptor delta constant) (0 活性数据)

点击查看靶蛋白信息： TRDC

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

TRGC2 Tdark T 细胞受体伽马常数 2(T cell receptor gamma constant 2) (0 活性数据)

点击查看靶蛋白信息： TRGC2

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant TCR γ/δ Antibody (FITC) (Ab216133)

Recombinant TCR γ/δ Antibody (FITC) (Ab216133) - Flow Cytometry
Two-color flow cytometric analysis of TCR γ/δ expression on human peripheral blood lymphocytes. Human peripheral blood lymphocytes were stained with Recombinant CD3 Antibody (APC) (Ab214240) and either Mouse IgG (FITC) (Ab180307; Left panel) or Recombinant TCR γ/δ Antibody (FITC) (Ab216133) at 5μL/test.

应用

应用名称	稀释比例
Flow Cytometry	5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.