Hsp27 Mouse mAb

COA

级别和纯度:

≥95%(SDS-PAGE)
1.0 mg/mL

应用:

ELISA
Flow Cytometry
IF/ICC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG2b
克隆号: CD05/2A2
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab156228-10μl	10μl	现货
Ab156228-50μl	50μL	现货
Ab156228-100μl	100μL	现货
Ab156228-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: HSPB1

Accession#:P04792 (5) Gene ID:3315 (5) Hsp27 (5) HSPB1 (5) 流式细胞术 (1163)

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基本信息

产品名称	Hsp27 Mouse mAb
别名	热休克蛋白27 \| Anti-Hsp27抗体 \| HSPB1小鼠单克隆抗体 \| 小鼠抗人Hsp27抗体 \| Hsp27抗体 \| HSPB1抗体
英文别名	Heat shock 27kDa protein antibody \| 28 kDa heat shock protein antibody \| CMT2F antibody \| DKFZp586P1322 antibody \| epididymis secretory protein Li 102 antibody \| Estrogen regulated 24 kDa protein antibody \| Estrogen-regulated 24 kDa protein antibody \| Hea
规格或纯度	ExactAb™, 已验证, 无载体, 无叠氮钠, ≥95%(SDS-PAGE), 1.0 mg/mL
生化机理	Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.
宿主种属	小鼠(Mouse)
特异性	Hsp27
种属反应性	人(Human)
预测反应种属	Macaca mulatta
免疫原	Recombinant protein corresponding to Human Hsp27
阳性对照	WB: MCF-7, HeLa, K562, A431, A549 and LNCaP whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
SDS-PAGE	150.0 KDa
纯化方法	Protein A purified
储存缓冲液	PBS, pH 7.2
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
分子类型	抗体

关联靶点（人）

HSPB1 Tchem 热休克蛋白β-1(Heat shock protein beta-1) (0 活性数据)

点击查看靶蛋白信息： HSPB1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Hsp27 Mouse mAb (Ab156228)

Hsp27 Mouse mAb (Ab156228) - Western Blot
All lanes: Hsp27 Mouse Antibody (Ab156228) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 27 kDa
Exposure time: 7.6 s

Flow Cytometry: Hsp27 Mouse mAb (Ab156228)

Hsp27 Mouse mAb (Ab156228) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with Hsp27 Mouse mAb (Ab156228) (Red) at 0.625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG2b was used as an isotype control (Blue).

ICC/IF: Hsp27 Mouse mAb (Ab156228)

Hsp27 Mouse mAb (Ab156228) - ICC/IF
IF analysis of Hsp27 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp27 Mouse mAb (Ab156228) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

应用

应用名称	稀释比例
IF/ICC	1/200
WB	1/1000 - 1/20000
Flow Cytometry	1/200 - 1/50000

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F1113742	分析证书	Ab156228
ZJ24F1113743	分析证书	Ab156228
ZJ24F1113741	分析证书	Ab156228

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