Hsp27 Mouse mAb

COA

级别和纯度:

见COA

应用:

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG2b
克隆号: D2
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab108837-10μl	10μl	现货
Ab108837-50μl	50μL	现货
Ab108837-100μl	100μL	期货
Ab108837-1ml	1ml	期货

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点击查看靶蛋白信息: HSPB1

Accession#:P04792 (5) Gene ID:3315 (5) Hsp27 (5) HSPB1 (5)

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基本信息

产品名称	Hsp27 Mouse mAb
别名	27kDa热休克蛋白(Hsp27)单克隆抗体 \| Hsp27 小鼠单克隆抗体 \| Hsp27 小鼠单抗
英文别名	Heat shock 27kDa protein antibody \| 28 kDa heat shock protein antibody \| CMT2F antibody \| DKFZp586P1322 antibody \| epididymis secretory protein Li 102 antibody \| Estrogen regulated 24 kDa protein antibody \| Estrogen-regulated 24 kDa protein antibody \| Hea
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属	小鼠(Mouse)
特异性	HSPB1
种属反应性	人(Human)
免疫原	Recombinant Hsp27 expressed in E.coli (AA 2-205)
阳性对照	WB: HeLa and HepG2 cell lysates.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	D2
Format	Whole IgG
亚型	Mouse IgG2b
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A + Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂	0.05% Proclin 300
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

HSPB1 Tchem 热休克蛋白β-1(Heat shock protein beta-1) (0 活性数据)

点击查看靶蛋白信息： HSPB1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Hsp27 Mouse mAb (Ab108837)

Hsp27 Mouse mAb (Ab108837) - Western Blot
All lanes: Hsp27 Mouse mAb (Ab108837) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 1.35 s

应用

应用名称	稀释比例
WB	0.01-2 µg/mL

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找到5个结果

批号(Lot Number)	证书类型	货号
ZJ23F1201909	分析证书	Ab108837
ZJ23F1201910	分析证书	Ab108837
ZJ23F1201911	分析证书	Ab108837
ZJ24F1011857	分析证书	Ab108837
ZJ24F1011807	分析证书	Ab108837

技术文档和文章

细胞内ELISA实验方案

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In-cell ELISA protocol

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In-cell ELISA protocol

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Monoclonal antibodies In-cell ELISA Cytoblot Protein quantification HRP-Conjugated Secondary Antibodies Polyclonal Antibodies

蛋白质印迹的一抗选择指南

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Guidelines of Primary Antibodies for Western Blotting

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Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

高分子量蛋白质印迹法

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Protocol of Western Blotting For High Molecular Weights

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

叠氮化钠去除实验

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引用文献

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15. Faucher, C C and 6 more authors.. (1993) The 28-kDa protein whose phosphorylation is induced by protein kinase C activators in MCF-7 cells belongs to the family of low molecular mass heat shock proteins and is the estrogen-regulated 24-kDa protein.. The Journal of biological chemistry, (15): [PMID:8325890]
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18. Liu, C C, Gilmont, R R RR, Benndorf, R R and Welsh, M J MJ.. (2000) Identification and characterization of a novel protein from Sertoli cells, PASS1, that associates with mammalian small stress protein hsp27.. The Journal of biological chemistry, (23): [PMID:10751411]
19. Hino, M M, Kurogi, K K, Okubo, M A MA, Murata-Hori, M M and Hosoya, H H.. (2000) Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells.. Biochemical and biophysical research communications, (29): [PMID:10777697]
20. Benndorf, R R and 8 more authors.. (2001) HSP22, a new member of the small heat shock protein superfamily, interacts with mimic of phosphorylated HSP27 ((3D)HSP27).. The Journal of biological chemistry, (20): [PMID:11342557]
21. Hillier, Ladeana W LW and 106 more authors.. (2003) The DNA sequence of human chromosome 7.. Nature, (10): [PMID:12853948]
22. Evgrafov, Oleg V OV and 25 more authors.. (2004) Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.. Nature genetics, [PMID:15122254]
23. De Souza, A I AI and 5 more authors.. (2005) Heat shock protein 27 is associated with freedom from graft vasculopathy after human cardiac transplantation.. Circulation research, (22): [PMID:15976317]
24. Leong, Wai Fook WF and Chow, Vincent T K VT.. (2006) Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection.. Cellular microbiology, [PMID:16548883]
25. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243]
26. Yu, Li-Rong LR and 5 more authors.. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.. Journal of proteome research, [PMID:17924679]
27. Zahedi, René P RP and 8 more authors.. (2008) Phosphoproteome of resting human platelets.. Journal of proteome research, [PMID:18088087]
28. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648]
29. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976]
30. Houlden, H H and 5 more authors.. (2008) Mutations in the HSP27 (HSPB1) gene cause dominant, recessive, and sporadic distal HMN/CMT type 2.. Neurology, (18): [PMID:18832141]
31. Ikeda, Yoshihisa Y and 5 more authors.. (2009) A clinical phenotype of distal hereditary motor neuronopathy type II with a novel HSPB1 mutation.. Journal of the neurological sciences, (15): [PMID:18952241]
32. Kostenko, Sergiy S, Johannessen, Mona M and Moens, Ugo U.. (2009) PKA-induced F-actin rearrangement requires phosphorylation of Hsp27 by the MAPKAP kinase MK5.. Cellular signalling, [PMID:19166925]
33. Vos, Michel J MJ, Kanon, Bart B and Kampinga, Harm H HH.. (2009) HSPB7 is a SC35 speckle resident small heat shock protein.. Biochimica et biophysica acta, [PMID:19464326]
34. Almeida-Souza, Leonardo L and 11 more authors.. (2010) Increased monomerization of mutant HSPB1 leads to protein hyperactivity in Charcot-Marie-Tooth neuropathy.. The Journal of biological chemistry, (23): [PMID:20178975]
35. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
36. Mi, Lixin and 8 more authors.. (2011) Identification of potential protein targets of isothiocyanates by proteomics.. Chemical research in toxicology, (17): [PMID:21838287]
37. Capponi, Simona S and 8 more authors.. (2011) HSPB1 and HSPB8 in inherited neuropathies: study of an Italian cohort of dHMN and CMT2 patients.. Journal of the peripheral nervous system : JPNS, [PMID:22176143]
38. Lin, Kon-Ping KP and 8 more authors.. (2011) The mutational spectrum in a cohort of Charcot-Marie-Tooth disease type 2 among the Han Chinese in Taiwan.. PloS one, [PMID:22206013]
39. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
40. Nefedova, Victoria V VV, Datskevich, Petr N PN, Sudnitsyna, Maria V MV, Strelkov, Sergei V SV and Gusev, Nikolai B NB.. (2013) Physico-chemical properties of R140G and K141Q mutants of human small heat shock protein HspB1 associated with hereditary peripheral neuropathies.. Biochimie, [PMID:23643870]
41. Holmgren, Anne A and 6 more authors.. (2013) Charcot-Marie-Tooth causing HSPB1 mutations increase Cdk5-mediated phosphorylation of neurofilaments.. Acta neuropathologica, [PMID:23728742]
42. Nefedova, Victoria V VV, Sudnitsyna, Maria V MV, Strelkov, Sergei V SV and Gusev, Nikolai B NB.. (2013) Structure and properties of G84R and L99M mutants of human small heat shock protein HspB1 correlating with motor neuropathy.. Archives of biochemistry and biophysics, (1): [PMID:23948568]
43. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315]
44. Muranova, Lydia K LK, Weeks, Stephen D SD, Strelkov, Sergei V SV and Gusev, Nikolai B NB.. (2015) Characterization of Mutants of Human Small Heat Shock Protein HspB1 Carrying Replacements in the N-Terminal Domain and Associated with Hereditary Motor Neuron Diseases.. PloS one, [PMID:25965061]
45. Echaniz-Laguna, Andoni A and 20 more authors.. (2017) Axonal Neuropathies due to Mutations in Small Heat Shock Proteins: Clinical, Genetic, and Functional Insights into Novel Mutations.. Human mutation, [PMID:28144995]
46. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

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体积（添加到小瓶中）

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所需的复溶浓度

Hsp27 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器