TIMP1 Antibody

COA

级别和纯度:

已验证

ExactAb™

2.6 mg/mL

应用:

宿主种属: 兔(Rabbit)
种属反应性: 人(Human)
亚型: Rabbit IgG
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab131327-10μl	10μl	现货
Ab131327-50μl	50μL	现货
Ab131327-100μl	100μL	期货
Ab131327-1ml	1ml	期货

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点击查看靶蛋白信息: TIMP1

Accession#:P01033 (1) Gene ID:7076 (1) Timp1 (3)

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基本信息

产品名称	TIMP1 Antibody
别名	TIMP1抗体 \| 金属蛋白酶组织抑制因子-1抗体 \| TIMP1抗体
英文别名	Clgi antibody \| Collagenase inhibitor antibody \| Collagenase inhibitor, Human antibody \| EPA antibody \| EPO antibody \| Erythroid Potentiating Activity antibody \| Erythroid-potentiating activity antibody \| Fibroblast collagenase inhibitor antibody \| FLJ903
规格或纯度	ExactAb™, Validated, 2.6 mg/mL
宿主种属	兔(Rabbit)
特异性	TIMP1
种属反应性	人(Human)
免疫原	A synthetic peptide derived from human TIMP1 (AA 1-207).
阳性对照	WB: HT-29, SK-OV-3 and HeLa cell lysates.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4
防腐剂	0.02% Sodium azide
浓度	2.6 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

TIMP1 Tbio 金属蛋白酶抑制剂1(Metalloproteinase inhibitor 1) (0 活性数据)

点击查看靶蛋白信息： TIMP1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: TIMP1 Antibody (Ab131327)

TIMP1 Antibody (Ab131327) - Western Blot
All lanes: TIMP1 Antibody (Ab131327) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 28 kDa
Exposure time: 1.0 s

应用

应用名称	稀释比例
WB	1/500-1/1000

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高分子量蛋白质印迹法

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

引用文献

1. O'Shea, M M and 7 more authors.. (1992) Site-directed mutations that alter the inhibitory activity of the tissue inhibitor of metalloproteinases-1: importance of the N-terminal region between cysteine 3 and cysteine 13.. Biochemistry, (27): [PMID:1420137]
2. Van Ranst, M M and 7 more authors.. (1991) The cytokine-protease connection: identification of a 96-kD THP-1 gelatinase and regulation by interleukin-1 and cytokine inducers.. Cytokine, [PMID:1653055]
3. Osthues, A A, Knäuper, V V, Oberhoff, R R, Reinke, H H and Tschesche, H H.. (1992) Isolation and characterization of tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) from human rheumatoid synovial fluid.. FEBS letters, (13): [PMID:1730286]
4. Williamson, R A RA and 9 more authors.. (1990) Disulphide bond assignment in human tissue inhibitor of metalloproteinases (TIMP).. The Biochemical journal, (1): [PMID:2163605]
5. Rapp, G G and 6 more authors.. (1990) Characterization of three abundant mRNAs from human ovarian granulosa cells.. DNA and cell biology, [PMID:2171551]
6. Carmichael, D F DF and 6 more authors.. (1986) Primary structure and cDNA cloning of human fibroblast collagenase inhibitor.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3010309]
7. Docherty, A J AJ and 7 more authors.. (1985) Sequence of human tissue inhibitor of metalloproteinases and its identity to erythroid-potentiating activity.. Nature, [PMID:3903517]
8. Opbroek, A A, Kenney, M C MC and Brown, D D.. (1993) Characterization of a human corneal metalloproteinase inhibitor (TIMP-1).. Current eye research, [PMID:7507419]
9. Knäuper, V V, López-Otin, C C, Smith, B B, Knight, G G and Murphy, G G.. (1996) Biochemical characterization of human collagenase-3.. The Journal of biological chemistry, (19): [PMID:8576151]
10. Knäuper, V V and 7 more authors.. (1997) The role of the C-terminal domain of human collagenase-3 (MMP-13) in the activation of procollagenase-3, substrate specificity, and tissue inhibitor of metalloproteinase interaction.. The Journal of biological chemistry, (21): [PMID:9065415]
11. Hardcastle, A J AJ, Thiselton, D L DL, Nayudu, M M, Hampson, R M RM and Bhattacharya, S S SS.. (1997) Genomic organization of the human TIMP-1 gene. Investigation of a causative role in the pathogenesis of X-linked retinitis pigmentosa 2.. Investigative ophthalmology & visual science, [PMID:9286280]
12. Gomis-Rüth, F X FX and 11 more authors.. (1997) Mechanism of inhibition of the human matrix metalloproteinase stromelysin-1 by TIMP-1.. Nature, (4): [PMID:9288970]
13. Wu, B B and 7 more authors.. (2000) NMR structure of tissue inhibitor of metalloproteinases-1 implicates localized induced fit in recognition of matrix metalloproteinases.. Journal of molecular biology, (14): [PMID:10623524]
14. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161]
15. Lewandrowski, Urs U, Moebius, Jan J, Walter, Ulrich U and Sickmann, Albert A.. (2006) Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach.. Molecular & cellular proteomics : MCP, [PMID:16263699]
16. Liu, Tao T and 6 more authors.. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952]
17. Ramachandran, Prasanna P and 5 more authors.. (2006) Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry.. Journal of proteome research, [PMID:16740002]
18. Jung, Ki-Kyung KK, Liu, Xu-Wen XW, Chirco, Rosemarie R, Fridman, Rafael R and Kim, Hyeong-Reh Choi HR.. (2006) Identification of CD63 as a tissue inhibitor of metalloproteinase-1 interacting cell surface protein.. The EMBO journal, (6): [PMID:16917503]
19. Iyer, Shalini S, Wei, Shuo S, Brew, Keith K and Acharya, K Ravi KR.. (2007) Crystal structure of the catalytic domain of matrix metalloproteinase-1 in complex with the inhibitory domain of tissue inhibitor of metalloproteinase-1.. The Journal of biological chemistry, (5): [PMID:17050530]
20. Grossman, Moran M and 6 more authors.. (2010) The intrinsic protein flexibility of endogenous protease inhibitor TIMP-1 controls its binding interface and affects its function.. Biochemistry, (27): [PMID:20545310]
21. Batra, Jyotica J and 5 more authors.. (2012) Matrix metalloproteinase-10 (MMP-10) interaction with tissue inhibitors of metalloproteinases TIMP-1 and TIMP-2: binding studies and crystal structure.. The Journal of biological chemistry, (4): [PMID:22427646]
22. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912]
23. Toricelli, Mariana M, Melo, Fabiana H M FH, Peres, Giovani B GB, Silva, Débora C P DC and Jasiulionis, Miriam G MG.. (2013) Timp1 interacts with beta-1 integrin and CD63 along melanoma genesis and confers anoikis resistance by activating PI3-K signaling pathway independently of Akt phosphorylation.. Molecular cancer, (25): [PMID:23522389]
24. Lee, Soo Youn SY and 9 more authors.. (2014) TIMP-1 modulates chemotaxis of human neural stem cells through CD63 and integrin signalling.. The Biochemical journal, (1): [PMID:24635319]
25. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039]
26. Gasson, J C JC and 9 more authors.. (1985) Molecular characterization and expression of the gene encoding human erythroid-potentiating activity.. Nature, [PMID:3839290]

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TIMP1 Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器