货号 (SKU)

包装规格

是否现货

价格

数量

Ab123181-10μl

10μl

现货

Ab123181-50μl

50μL

现货

Ab123181-100μl

100μL

现货

Ab123181-1ml

1ml

期货

基本信息

产品名称	Profilin 1 Antibody
别名	Profilin 1抗体 \| 抑丝蛋白1抗体 \| 原肌球蛋白 1 抗体
英文别名	Actin binding proteinantibody \| ALS18 antibody \| Epididymis tissue protein Li 184a antibody \| OTTHUMP00000125244 antibody \| PFN 1 antibody \| Pfn antibody \| PFN1 antibody \| PROF1_HUMAN antibody \| Profilin I antibody \| Profilin-1 antibody \| Profilin1 antibo
规格或纯度	无载体, ExactAb™, 已验证, 高性能, 见COA
宿主种属	兔(Rabbit)
特异性	Profilin 1
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	Recombinant PFN1 expressed in E.coli (AA 1-139) .
阳性对照	WB: HeLa, Jurkat, HEK293, HUVEC, Hep G2, NIH/3T3 and C6 cell lysates.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Antigen affinity purified + Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH 7.4.
防腐剂	0.02% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

PFN1 Tbio 抑制蛋白-1(Profilin-1) (0 活性数据)

点击查看靶蛋白信息： PFN1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: Profilin 1 Antibody (Ab123181)

Profilin 1 Antibody (Ab123181) - Western Blot
All lanes: Profilin 1 Antibody (Ab123181) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 10.0 s

应用

应用名称	稀释比例
WB	0.01-2 µg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0303645	分析证书	Ab123181
ZJ24F0303644	分析证书	Ab123181
ZJ24F0303643	分析证书	Ab123181

技术文档和文章

细胞内ELISA实验方案

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单克隆抗体多克隆抗体细胞内酶联免疫吸附实验细胞印迹蛋白定量辣根过氧化物酶标记二抗

In-cell ELISA protocol

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Monoclonal antibodies In-cell ELISA Cytoblot Protein quantification HRP-Conjugated Secondary Antibodies Polyclonal Antibodies

In-cell ELISA protocol

Categories: 实验方案（Protocols）

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Monoclonal antibodies In-cell ELISA Cytoblot Protein quantification HRP-Conjugated Secondary Antibodies Polyclonal Antibodies

蛋白质印迹的一抗选择指南

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重组抗体单克隆抗体多克隆抗体蛋白质印迹内参抗体一抗选择

Guidelines of Primary Antibodies for Western Blotting

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Guidelines of Primary Antibodies for Western Blotting

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Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

高分子量蛋白质印迹法

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Protocol of Western Blotting For High Molecular Weights

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

可替换产品

引用文献

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
3. Ampe, C C, Markey, F F, Lindberg, U U and Vandekerckhove, J J.. (1988) The primary structure of human platelet profilin: reinvestigation of the calf spleen profilin sequence.. FEBS letters, (8): [PMID:3342873]
4. Kwiatkowski, D J DJ and Bruns, G A GA.. (1988) Human profilin. Molecular cloning, sequence comparison, and chromosomal analysis.. The Journal of biological chemistry, (25): [PMID:3356709]
5. Gieselmann, R R, Kwiatkowski, D J DJ, Janmey, P A PA and Witke, W W.. (1995) Distinct biochemical characteristics of the two human profilin isoforms.. European journal of biochemistry, (1): [PMID:7758455]
6. Metzler, W J WJ and 6 more authors.. (1993) Characterization of the three-dimensional solution structure of human profilin: 1H, 13C, and 15N NMR assignments and global folding pattern.. Biochemistry, (21): [PMID:8268157]
7. Mahoney, N M NM, Janmey, P A PA and Almo, S C SC.. (1997) Structure of the profilin-poly-L-proline complex involved in morphogenesis and cytoskeletal regulation.. Nature structural biology, [PMID:9360613]
8. Mahoney, N M NM, Rozwarski, D A DA, Fedorov, E E, Fedorov, A A AA and Almo, S C SC.. (1999) Profilin binds proline-rich ligands in two distinct amide backbone orientations.. Nature structural biology, [PMID:10404225]
9. Stüven, Theis T, Hartmann, Enno E and Görlich, Dirk D.. (2003) Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes.. The EMBO journal, (3): [PMID:14592989]
10. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843]
11. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455]
12. Ferron, François F, Rebowski, Grzegorz G, Lee, Sung Haeng SH and Dominguez, Roberto R.. (2007) Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena/VASP.. The EMBO journal, (31): [PMID:17914456]
13. Shao, Jieya J, Welch, William J WJ, Diprospero, Nicholas A NA and Diamond, Marc I MI.. (2008) Phosphorylation of profilin by ROCK1 regulates polyglutamine aggregation.. Molecular and cellular biology, [PMID:18573880]
14. Baek, Kyuwon K and 5 more authors.. (2008) Modulation of actin structure and function by phosphorylation of Tyr-53 and profilin binding.. Proceedings of the National Academy of Sciences of the United States of America, (19): [PMID:18689676]
15. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
16. Li, JianYuan J and 12 more authors.. (2010) Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins.. Molecular & cellular proteomics : MCP, [PMID:20736409]
17. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
18. Wu, Chi-Hong CH and 37 more authors.. (2012) Mutations in the profilin 1 gene cause familial amyotrophic lateral sclerosis.. Nature, (23): [PMID:22801503]
19. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Profilin 1 Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器

Profilin 1 Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器