Profilin 1 Mouse mAb

COA

级别和纯度:

≥95%(SDS-PAGE)
1.0 mg/mL

应用:

Flow Cytometry
IF/ICC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG2b
克隆号: AB01/2E11
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab191887-10μl	10μl	现货
Ab191887-50μl	50μL	现货
Ab191887-100μl	100μL	现货
Ab191887-1ml	1ml	现货

查看相关系列

点击查看靶蛋白信息: PFN1

Accession#:P07737 (11) Gene ID:5216 (11) PFN1 (11) Profilin 1 (11) 流式细胞术 (1163)

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基本信息

产品名称	Profilin 1 Mouse mAb
别名	前纤维蛋白1抗体 \| Anti-Profilin 1抗体 \| Profilin 1小鼠单克隆抗体 \| PFN1 小鼠单克隆抗体 \| PFN1抗体 \| 原肌球蛋白 1 小鼠单克隆抗体 \| 原肌球蛋白 1 小鼠单抗
英文别名	Actin binding protein antibody \| ALS18 antibody \| Epididymis tissue protein Li 184a antibody \| OTTHUMP00000125244 antibody \| PFN 1 antibody \| Pfn antibody \| PFN1 antibody \| PROF1_HUMAN antibody \| Profilin I antibody \| Profilin-1 antibody \| ProfilinI antib
规格或纯度	ExactAb™, Validated, 无动物源, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE), 1.0 mg/mL
生化机理	与肌动蛋白结合，影响细胞骨架的结构。在高浓度下，肌动蛋白会阻止肌动蛋白的聚合，而在低浓度下则会增强肌动蛋白的聚合。通过与 PIP2 结合，它可抑制 IP3 和 DG 的形成。抑制雄激素受体（AR）和 HTT 的聚合，G-肌动蛋白的结合是其抑制 AR 的关键。
宿主种属	小鼠(Mouse)
特异性	Profilin 1
种属反应性	人(Human)
预测反应种属	Pongo pygmaeus,Symphalangus syndactylus,Theropithecus gelada
免疫原	Recombinant protein corresponding to Human Profilin 1
阳性对照	WB: HeLa, HepG2, HT-29, K562, HEK293 and Jurkat whole cell lysates Flow: HeLa cells IF/ICC: U-2 OS cells
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	AB01/2E11
Format	Whole IgG
亚型	Mouse IgG2b
轻链亚型	lambda
SDS-PAGE	150.0 KDa
纯化方法	Protein A purified
储存缓冲液	PBS, pH 7.2
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

PFN1 Tbio 抑制蛋白-1(Profilin-1) (0 活性数据)

点击查看靶蛋白信息： PFN1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: Profilin 1 Mouse mAb (Ab191887)

Profilin 1 Mouse mAb (Ab191887) - Western Blot
All lanes: Profilin 1 Mouse Antibody (Ab191887) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 23.4 s

Western Blot: Profilin 1 Mouse mAb (Ab191887)

Flow Cytometry: Profilin 1 Mouse mAb (Ab191887)

Profilin 1 Mouse mAb (Ab191887) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with Profilin 1 Mouse mAb (Ab191887) (Red) at 1.25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG2b was used as an isotype control (Blue).

ICC/IF: Profilin 1 Mouse mAb (Ab191887)

Profilin 1 Mouse mAb (Ab191887) - ICC/IF
IF analysis of Profilin 1 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Profilin 1 Mouse mAb (Ab191887) at 0.5 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

应用