货号 (SKU)

包装规格

是否现货

价格

数量

Ab120681-10μl

10μl

现货

Ab120681-50μl

50μL

现货

Ab120681-100μl

100μL

现货

Ab120681-1ml

1ml

期货

基本信息

产品名称	Recombinant PCNA Antibody
别名	增殖细胞核抗原(内参)重组单克隆抗体 \| 增殖细胞核抗原重组单克隆抗体 \| 增殖细胞核抗原抗体 \| 重组PCNA抗体
英文别名	ATLD2 antibody \| cb16 antibody \| Cyclin antibody \| DNA polymerase delta auxiliary protein antibody \| etID36690.10 antibody \| fa28e03 antibody \| fb36g03 antibody \| HGCN8729 antibody \| MGC8367 antibody \| Mutagen-sensitive 209 protein antibody \| OTTHUMP00000
规格或纯度	ExactAb™, Validated, Recombinant, 0.5 mg/mL
宿主种属	兔(Rabbit)
特异性	PCNA
种属反应性	人(Human),大鼠(Rat),牛(Cow),狗(Dog),绿猴(Green Monkey),鸡(Chicken)
交叉反应	Cross-reactivity with other species has not been measured.
免疫原	A synthetic peptide corresponding to residues of human PCNA was used as an immunogen (AA 100-200).
阳性对照	WB: A431, HEK293, MCF7, HeLa, Hep G2, NIH/3T3, C6, K562, Jurkat and HUVEC cell lysates. Flow: HeLa cells. IP: HeLa whole cell lysate. IF/ICC: HeLa cells.
偶联	Unconjugated

产品属性

克隆类型	重组抗体
克隆号	RR704
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3
防腐剂	0.01% Sodium azide
浓度	0.5 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

PCNA Tchem 增殖细胞核抗原(Proliferating cell nuclear antigen) (0 活性数据)

点击查看靶蛋白信息： PCNA

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Recombinant PCNA Antibody (Ab120681)

Recombinant PCNA Antibody (Ab120681) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution

Predicted band size: 29 kDa
Observed band size: 32 kDa
Exposure time: 14.1 s

ICC/IF : Recombinant PCNA Antibody (Ab120681)

Recombinant PCNA Antibody (Ab120681) - ICC/IF
IF analysis of PCNA (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant PCNA Antibody (Ab120681) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Recombinant PCNA Antibody (Ab120681)

Recombinant PCNA Antibody (Ab120681) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant PCNA Antibody (Ab120681) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant PCNA Antibody (Ab120681) (1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.

IP: Recombinant PCNA Antibody (Ab120681)

Recombinant PCNA Antibody (Ab120681) - IP
PCNA was immunoprecipitated from 0.4 mg of HeLa whole cell lysate with Recombinant PCNA Antibody (Ab120681) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant PCNA Antibody (Ab120681) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant PCNA Antibody (Ab120681) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input).

应用

应用名称	稀释比例
Flow Cytometry	1/800 - 1/2000
IP	1/50
WB	1/1000 - 1/2000
IF/ICC	1/1000

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F0901113	分析证书	Ab120681
ZJ23F0901112	分析证书	Ab120681
ZJ24F1011773	分析证书	Ab120681

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