P4HB Antibody

COA

级别和纯度:

见COA

应用:

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Rabbit IgG
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab119767-10μl	10μl	现货
Ab119767-50μl	50μL	现货
Ab119767-100μl	100μL	现货
Ab119767-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: P4HB

Accession#:P07237 (2) Gene ID:5034 (2) P4HB (2)

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基本信息

产品名称	P4HB Antibody
别名	蛋白二硫键异构酶(PDI)多克隆抗体 \| P4HB多克隆抗体 \| P4HB抗体
英文别名	Cellular thyroid hormone-binding protein \| collagen prolyl 4-hydroxylase beta \| DSI \| ERBA2L \| GIT \| glutathione-insulin transhydrogenase \| P4HB \| P4Hbeta \| p55 \| PDI \| PDIA1 \| PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属	兔(Rabbit)
特异性	P4HB
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	Recombinant PDI expressed in E.coli (AA 216-465)
阳性对照	WB: Hep G2, MCF7, HEK293, A431 and HeLa cell lysates.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150kDa
纯化方法	Antigen affinity purified and protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂	0.05% Proclin 300
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

P4HB Tchem 蛋白质二硫键异构酶(Protein disulfide-isomerase) (0 活性数据)

点击查看靶蛋白信息： P4HB

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: P4HB Antibody (Ab119767)

P4HB Antibody (Ab119767) - Western Blot
All lanes: P4HB Antibody (Ab119767) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 59 kDa
Exposure time: 3.4 s

应用

应用名称	稀释比例
WB	0.5-2 µg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F1001373	分析证书	Ab119767
ZJ23F1001374	分析证书	Ab119767
ZJ24F1011791	分析证书	Ab119767

技术文档和文章

石蜡样品的免疫组化染色方案（IHC-P）

Categories: 实验方案（Protocols）

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单克隆抗体石蜡切片免疫组化染色

蛋白质印迹的一抗选择指南

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重组抗体单克隆抗体多克隆抗体蛋白质印迹内参抗体一抗选择

Guidelines of Primary Antibodies for Western Blotting

Categories: 实验方案（Protocols）

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Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

Guidelines of Primary Antibodies for Western Blotting

Categories: 实验方案（Protocols）

探索主题:

Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

高分子量蛋白质印迹法

Categories: 实验方案（Protocols）

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免疫印迹生物缓冲液蛋白质印迹高分子量蛋白大分子蛋白蛋白质电泳 WB抗体

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

引用文献

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3. Hayes KE, Batsomboon P, Chen WC, Johnson BD, Becker A, Eschrich S, Yang Y, Robart AR, Dudley GB, Geldenhuys WJ, Hazlehurst LA.. (2019) Inhibition of the FAD containing ER oxidoreductin 1 (Ero1) protein by EN-460 as a strategy for treatment of multiple myeloma.. Bioorg Med Chem, 27 (8): (1479-1488). [PMID:30850265] [10.1016/j.bmc.2019.02.016]
4. Hochstrasser, D F DF and 9 more authors.. (1992) Human liver protein map: a reference database established by microsequencing and gel comparison.. Electrophoresis, [PMID:1286669]
5. Tasanen, K K, Oikarinen, J J, Kivirikko, K I KI and Pihlajaniemi, T T.. (1992) Promoter of the gene for the multifunctional protein disulfide isomerase polypeptide. Functional significance of the six CCAAT boxes and other promoter elements.. The Journal of biological chemistry, (5): [PMID:1597478]
6. Bauw, G G and 7 more authors.. (1990) Two-dimensional gel electrophoresis, protein electroblotting and microsequencing: a direct link between proteins and genes.. Electrophoresis, [PMID:1699755]
7. Ward, L D LD, Hong, J J, Whitehead, R H RH and Simpson, R J RJ.. (1990) Development of a database of amino acid sequences for human colon carcinoma proteins separated by two-dimensional polyacrylamide gel electrophoresis.. Electrophoresis, [PMID:2079031]
8. Tasanen, K K, Parkkonen, T T, Chow, L T LT, Kivirikko, K I KI and Pihlajaniemi, T T.. (1988) Characterization of the human gene for a polypeptide that acts both as the beta subunit of prolyl 4-hydroxylase and as protein disulfide isomerase.. The Journal of biological chemistry, (5): [PMID:2846539]
9. Pihlajaniemi, T T and 6 more authors.. (1987) Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene.. The EMBO journal, [PMID:3034602]
10. Morris, J I JI and Varandani, P T PT.. (1988) Characterization of a cDNA for human glutathione-insulin transhydrogenase (protein-disulfide isomerase/oxidoreductase).. Biochimica et biophysica acta, (28): [PMID:3342239]
11. Cheng, S Y SY and 6 more authors.. (1987) The nucleotide sequence of a human cellular thyroid hormone binding protein present in endoplasmic reticulum.. The Journal of biological chemistry, (15): [PMID:3611107]
12. Helaakoski, T T and 5 more authors.. (1995) Cloning, baculovirus expression, and characterization of a second mouse prolyl 4-hydroxylase alpha-subunit isoform: formation of an alpha 2 beta 2 tetramer with the protein disulfide-isomerase/beta subunit.. Proceedings of the National Academy of Sciences of the United States of America, (9): [PMID:7753822]
13. Kemmink, J J, Darby, N J NJ, Dijkstra, K K, Scheek, R M RM and Creighton, T E TE.. (1995) Nuclear magnetic resonance characterization of the N-terminal thioredoxin-like domain of protein disulfide isomerase.. Protein science : a publication of the Protein Society, [PMID:8580850]
14. Kemmink, J J, Darby, N J NJ, Dijkstra, K K, Nilges, M M and Creighton, T E TE.. (1996) Structure determination of the N-terminal thioredoxin-like domain of protein disulfide isomerase using multidimensional heteronuclear 13C/15N NMR spectroscopy.. Biochemistry, (18): [PMID:8672469]
15. Ji, H H and 5 more authors.. A two-dimensional gel database of human colon carcinoma proteins.. Electrophoresis, [PMID:9150948]
16. Urade, R R and 5 more authors.. (1997) Functions of characteristic Cys-Gly-His-Cys (CGHC) and Gln-Glu-Asp-Leu (QEDL) motifs of microsomal ER-60 protease.. Journal of biochemistry, [PMID:9399589]
17. Kemmink, J J and 6 more authors.. (1999) The structure in solution of the b domain of protein disulfide isomerase.. Journal of biomolecular NMR, [PMID:10383197]
18. Mezghrani, A A and 5 more authors.. (2000) Protein-disulfide isomerase (PDI) in FRTL5 cells. pH-dependent thyroglobulin/PDI interactions determine a novel PDI function in the post-endoplasmic reticulum of thyrocytes.. The Journal of biological chemistry, (21): [PMID:10636893]
19. Fenouillet, E E, Barbouche, R R, Courageot, J J and Miquelis, R R.. (2001) The catalytic activity of protein disulfide isomerase is involved in human immunodeficiency virus envelope-mediated membrane fusion after CD4 cell binding.. The Journal of infectious diseases, (1): [PMID:11181151]
20. Mezghrani, A A and 5 more authors.. (2001) Manipulation of oxidative protein folding and PDI redox state in mammalian cells.. The EMBO journal, (15): [PMID:11707400]
21. Ko, Han Seok HS, Uehara, Takashi T and Nomura, Yasuyuki Y.. (2002) Role of ubiquilin associated with protein-disulfide isomerase in the endoplasmic reticulum in stress-induced apoptotic cell death.. The Journal of biological chemistry, (20): [PMID:12095988]
22. Gallina, Angelo A and 6 more authors.. (2002) Inhibitors of protein-disulfide isomerase prevent cleavage of disulfide bonds in receptor-bound glycoprotein 120 and prevent HIV-1 entry.. The Journal of biological chemistry, (27): [PMID:12218051]
23. Barbouche, Rym R, Miquelis, Raymond R, Jones, Ian M IM and Fenouillet, Emmanuel E.. (2003) Protein-disulfide isomerase-mediated reduction of two disulfide bonds of HIV envelope glycoprotein 120 occurs post-CXCR4 binding and is required for fusion.. The Journal of biological chemistry, (31): [PMID:12218052]
24. Lumb, Richard A RA and Bulleid, Neil J NJ.. (2002) Is protein disulfide isomerase a redox-dependent molecular chaperone?. The EMBO journal, (16): [PMID:12485997]
25. Basrur, Venkatesha V and 12 more authors.. Proteomic analysis of early melanosomes: identification of novel melanosomal proteins.. Journal of proteome research, [PMID:12643545]
26. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801]
27. She, Yi-Min YM and 5 more authors.. (2003) Identification of metal-binding proteins in human hepatoma lines by immobilized metal affinity chromatography and mass spectrometry.. Molecular & cellular proteomics : MCP, [PMID:14534351]
28. Markovic, Ingrid I and 8 more authors.. (2004) Thiol/disulfide exchange is a prerequisite for CXCR4-tropic HIV-1 envelope-mediated T-cell fusion during viral entry.. Blood, (1): [PMID:14592831]
29. Wilkinson, Bonney B and Gilbert, Hiram F HF.. (2004) Protein disulfide isomerase.. Biochimica et biophysica acta, (1): [PMID:15158710]
30. Barbouche, Rym R, Lortat-Jacob, Hugues H, Jones, Ian M IM and Fenouillet, Emmanuel E.. (2005) Glycosaminoglycans and protein disulfide isomerase-mediated reduction of HIV Env.. Molecular pharmacology, [PMID:15644496]
31. Chi, An A and 16 more authors.. (2006) Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.. Journal of proteome research, [PMID:17081065]
32. Bi, Shuguang S, Hong, Patrick W PW, Lee, Benhur B and Baum, Linda G LG.. (2011) Galectin-9 binding to cell surface protein disulfide isomerase regulates the redox environment to enhance T-cell migration and HIV entry.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:21670307]
33. Khatun, Irani I, Walsh, Meghan T MT and Hussain, M Mahmood MM.. (2013) Loss of both phospholipid and triglyceride transfer activities of microsomal triglyceride transfer protein in abetalipoproteinemia.. Journal of lipid research, [PMID:23475612]
34. Ge, Jingyan J and 7 more authors.. (2013) Small molecule probe suitable for in situ profiling and inhibition of protein disulfide isomerase.. ACS chemical biology, (15): [PMID:24070012]
35. Rauch, Frank F and 8 more authors.. (2015) Cole-Carpenter syndrome is caused by a heterozygous missense mutation in P4HB.. American journal of human genetics, (5): [PMID:25683117]
36. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039]
37. Walsh, Meghan T MT and 8 more authors.. (2015) Novel Abetalipoproteinemia Missense Mutation Highlights the Importance of the N-Terminal β-Barrel in Microsomal Triglyceride Transfer Protein Function.. Circulation. Cardiovascular genetics, [PMID:26224785]

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体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

P4HB Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器