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p46 Antibody

    应用:
  • IHC
  • WB
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
  • 亚型: Rabbit IgG
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab119753-10μl
10μl 现货 Stock Image
Ab119753-50μl
50μL 现货 Stock Image
Ab119753-100μl
100μL 期货 Stock Image
Ab119753-1ml
1ml 期货 Stock Image

基本信息

产品名称 p46 Antibody
别名 DNA聚合酶δ相互作用蛋白3抗体 | p46 抗体
英文别名 46 kDa DNA polymerase delta interaction protein | p46 | S6K1 Aly/REF-like target | SKAR | 6 kDa DNA polymerase delta interaction protein antibody | DNA polymerase delta interacting protein 3 antibody | KIAA1649 antibody | P46 antibody | PDIP3_HUMAN antibo
规格或纯度 ExactAb™, Validated, Carrier Free, 0.6 mg/mL
宿主种属 兔(Rabbit)
特异性 POLDIP3
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse)
免疫原 Fusion protein of human POLDIP3 (AA 36-229)
阳性对照 WB: HeLa, Hep G2, Jurkat, HEK293, RAW 264.7 and C6 cell lysates. IHC: Human thyroid cancer tissue.
偶联 Unconjugated

产品属性

克隆类型 多克隆抗体
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 10mM PBS, 40% Glycerol, 0.05% Sodium azide, pH 7.4
防腐剂 0.05% Sodium azide
浓度 0.6 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

POLDIP3 Tbio 聚合酶 delta 相互作用蛋白 3(Polymerase delta-interacting protein 3) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

p46 Antibody (Ab119753) - Western Blot
All lanes: p46 Antibody (Ab119753) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 46 kDa
Observed band size: 44, 47 kDa
Exposure time: 55.2 s

p46 Antibody (Ab119753) - Western Blot
All lanes: p46 Antibody (Ab119753) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 46 kDa
Observed band size: 44, 47 kDa
Exposure time: 73.3 s

p46 Antibody (Ab119753) - IHC
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using p46 Antibody (Ab119753). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

应用

应用名称 稀释比例
WB

1/500-1/1000

IHC

1/50 - 1/100

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到2个结果

批号(Lot Number) 证书类型 货号
ZJ24F0404014 分析证书 Ab119753
ZJ24F0404013 分析证书 Ab119753

引用文献

1. Dunham, I I and 24 more authors..  (1999)  The DNA sequence of human chromosome 22..  Nature,  (2): [PMID:10591208]
2. Hirosawa, M M, Nagase, T T, Murahashi, Y Y, Kikuno, R R and Ohara, O O..  (2001)  Identification of novel transcribed sequences on human chromosome 22 by expressed sequence tag mapping..  DNA research : an international journal for rapid publication of reports on genes and genomes,  (28): [PMID:11258795]
3. Liu, Li L, Rodriguez-Belmonte, Esther M EM, Mazloum, Nayef N, Xie, Bin B and Lee, Marietta Y W T MY..  (2003)  Identification of a novel protein, PDIP38, that interacts with the p50 subunit of DNA polymerase delta and proliferating cell nuclear antigen..  The Journal of biological chemistry,  (21): [PMID:12522211]
4. Collins, John E JE and 8 more authors..  (2003)  Reevaluating human gene annotation: a second-generation analysis of chromosome 22..  Genome research,  [PMID:12529303]
5. Richardson, Celeste J CJ and 6 more authors..  (2004)  SKAR is a specific target of S6 kinase 1 in cell growth control..  Current biology : CB,  (7): [PMID:15341740]
6. Collins, John E JE and 12 more authors..  (2004)  A genome annotation-driven approach to cloning the human ORFeome..  Genome biology,  [PMID:15461802]
7. Smyk, Amelia A, Szuminska, Magdalena M, Uniewicz, Katarzyna A KA, Graves, Lee M LM and Kozlowski, Piotr P..  (2006)  Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase delta and S6K1 and regulating cell growth..  The FEBS journal,  [PMID:16984396]
8. Matsuoka, Shuhei S and 12 more authors..  (2007)  ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage..  Science (New York, N.Y.),  (25): [PMID:17525332]
9. Yu, Li-Rong LR and 5 more authors..  (2007)  Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra..  Journal of proteome research,  [PMID:17924679]
10. Ma, Xiaoju Max XM, Yoon, Sang-Oh SO, Richardson, Celeste J CJ, Jülich, Kristina K and Blenis, John J..  (2008)  SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs..  Cell,  (18): [PMID:18423201]
11. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,  (18): [PMID:19690332]
12. Dufu, Kobina K and 5 more authors..  (2010)  ATP is required for interactions between UAP56 and two conserved mRNA export proteins, Aly and CIP29, to assemble the TREX complex..  Genes & development,  (15): [PMID:20844015]
13. Bienvenut, Willy V WV and 6 more authors..  (2012)  Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features..  Molecular & cellular proteomics : MCP,  [PMID:22223895]
14. Van Damme, Petra P and 15 more authors..  (2012)  N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB..  Proceedings of the National Academy of Sciences of the United States of America,  (31): [PMID:22814378]
15. Folco, Eric G EG, Lee, Chung-Sheng CS, Dufu, Kobina K, Yamazaki, Tomohiro T and Reed, Robin R..  (2012)  The proteins PDIP3 and ZC11A associate with the human TREX complex in an ATP-dependent manner and function in mRNA export..  PloS one,  [PMID:22928037]
16. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,  (4): [PMID:23186163]
17. Guo, Ailan and 15 more authors..  (2014)  Immunoaffinity enrichment and mass spectrometry analysis of protein methylation..  Molecular & cellular proteomics : MCP,  [PMID:24129315]

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