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Recombinant MDA5 Antibody

Recombinant MDA5 Antibody

级别和纯度:

见COA

应用:

宿主种属: 兔(Rabbit)
种属反应性: 人(Human)
亚型: Rabbit IgG
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab114669-10μl	10μl	现货
Ab114669-50μl	50μL	现货
Ab114669-100μl	100μL	现货
Ab114669-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: IFIH1

Accession#:Q9BYX4 (1) Gene ID:64135 (1) IFIH1 (1) MDA5 (1)

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基本信息

产品名称	Recombinant MDA5 Antibody
别名	Anti-MDA5抗体 \| MDA5抗体 \| Anti-MDA5抗体 \| MDA5抗体 \| 重组MDA5抗体 \| 黑色素瘤分化相关蛋白5抗体 \| 黑色素瘤分化相关蛋白5单克隆抗体 \| 黑色素瘤分化相关蛋白5重组单克隆抗体 \| MDA5抗体 \| MDA5单克隆抗体 \| MDA5重组单克隆抗体 \| IFIH1抗体 \| IFIH1单克隆抗体 \| IFIH1重组单克隆抗体
英文别名	IFIH1 \| AGS7 \| Hlcd \| IDDM19 \| IMD95 \| MDA-5 \| MDA5 \| RLR-2 \| SGMRT1 \| CADM-140 autoantigen antibody \| Clinically amyopathic dermatomyositis autoantigen 140 kDa antibody \| DEAD/H (Asp Glu Ala Asp/His) box polypeptide antibody \| DEAD/H box polypeptide anti
规格或纯度	重组, ExactAb™, 已验证, 敲低验证, 见COA
生化机理	RNA helicase that, through its ATP-dependent unwinding of RNA, may function to promote message degradation by specific RNases. Seems to have growth suppressive properties. Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). ATPase activity is specifically induced by dsRNA. Essential for the production of interferons in response to picornaviruses.
宿主种属	兔(Rabbit)
特异性	IFIH1
种属反应性	人(Human)
免疫原	A synthetic peptide derived from human MDA5 (AA 950-1025)
阳性对照	WB: MCF7, Jurkat and Hep G2 cell lysates.
偶联	Unconjugated

产品属性

克隆类型	重组抗体
Format	Whole IgG
亚型	Rabbit IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4
防腐剂	0.02% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融。
分子类型	抗体

关联靶点（人）

IFIH1 Tbio 干扰素诱导解旋酶C结构域蛋白1(Interferon-induced helicase C domain-containing protein 1) (0 活性数据)

点击查看靶蛋白信息： IFIH1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Recombinant MDA5 Antibody (Ab114669)

Recombinant MDA5 Antibody (Ab114669) - Western Blot
All lanes: Recombinant MDA5 Antibody (Ab114669) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 128 kDa
Exposure time: 120.0 s

Western Blot: Recombinant MDA5 Antibody (Ab114669)

Recombinant MDA5 Antibody (Ab114669) - Western Blot
All lanes use Recombinant MDA5 Antibody (Ab114669) at 1/1000 dilution for 1 hour at room temperature.

应用

应用名称	稀释比例
WB	1/1000 - 1/2000

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C of A & Other Certificates(BSE/TSE, COO):

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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到6个结果

批号(Lot Number)	证书类型	货号
ZJ25F0724496	分析证书	Ab114669
ZJ25F0724495	分析证书	Ab114669
ZJ25F0724494	分析证书	Ab114669
ZJ25F0521887	分析证书	Ab114669
ZJ24F0505717	分析证书	Ab114669
ZJ24F0505716	分析证书	Ab114669

技术文档和文章

蛋白质印迹的一抗选择指南

Categories: 实验方案（Protocols）

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重组抗体单克隆抗体多克隆抗体蛋白质印迹内参抗体一抗选择

Guidelines of Primary Antibodies for Western Blotting

Categories: 实验方案（Protocols）

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Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

Guidelines of Primary Antibodies for Western Blotting

Categories: 实验方案（Protocols）

探索主题:

Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

高分子量蛋白质印迹法

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免疫印迹生物缓冲液蛋白质印迹高分子量蛋白大分子蛋白蛋白质电泳 WB抗体

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

引用文献

1. Cui J, Zhu L, Xia X, Wang HY, Legras X, Hong J, Ji J, Shen P, Zheng S, Chen ZJ et al.. (2010) NLRC5 negatively regulates the NF-kappaB and type I interferon signaling pathways.. Cell, 141 (3): (483-96). [PMID:20434986]
2. Mackman RL, Mish M, Chin G, Perry JK, Appleby T, Aktoudianakis V, Metobo S, Pyun P, Niu C, Daffis S et al.. (2020) Discovery of GS-9688 (Selgantolimod) as a Potent and Selective Oral Toll-Like Receptor 8 Agonist for the Treatment of Chronic Hepatitis B.. J Med Chem, 63 (18): (10188-10203). [PMID:32407112]
3. Kang, Dong-chul DC and 5 more authors.. (2002) mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties.. Proceedings of the National Academy of Sciences of the United States of America, (22): [PMID:11805321]
4. Kovacsovics, Magdalena M and 5 more authors.. (2002) Overexpression of Helicard, a CARD-containing helicase cleaved during apoptosis, accelerates DNA degradation.. Current biology : CB, (14): [PMID:12015121]
5. Cocude, C C and 7 more authors.. (2003) A novel cellular RNA helicase, RH116, differentially regulates cell growth, programmed cell death and human immunodeficiency virus type 1 replication.. The Journal of general virology, [PMID:14645903]
6. Andrejeva, J J and 6 more authors.. (2004) The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter.. Proceedings of the National Academy of Sciences of the United States of America, (7): [PMID:15563593]
7. Kawai, Taro T and 8 more authors.. (2005) IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction.. Nature immunology, [PMID:16127453]
8. Meylan, Etienne E and 6 more authors.. (2005) Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus.. Nature, (20): [PMID:16177806]
9. Smyth, Deborah J DJ and 13 more authors.. (2006) A genome-wide association study of nonsynonymous SNPs identifies a type 1 diabetes locus in the interferon-induced helicase (IFIH1) region.. Nature genetics, [PMID:16699517]
10. Arimoto, Kei-ichiro K and 5 more authors.. (2007) Negative regulation of the RIG-I signaling by the ubiquitin ligase RNF125.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:17460044]
11. Diao, Feici F and 11 more authors.. (2007) Negative regulation of MDA5- but not RIG-I-mediated innate antiviral signaling by the dihydroxyacetone kinase.. Proceedings of the National Academy of Sciences of the United States of America, (10): [PMID:17600090]
12. Jounai, Nao N and 10 more authors.. (2007) The Atg5 Atg12 conjugate associates with innate antiviral immune responses.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:17709747]
13. Bamming, Darja D and Horvath, Curt M CM.. (2009) Regulation of signal transduction by enzymatically inactive antiviral RNA helicase proteins MDA5, RIG-I, and LGP2.. The Journal of biological chemistry, (10): [PMID:19211564]
14. Takahasi, Kiyohiro K and 10 more authors.. (2009) Solution structures of cytosolic RNA sensor MDA5 and LGP2 C-terminal domains: identification of the RNA recognition loop in RIG-I-like receptors.. The Journal of biological chemistry, (26): [PMID:19380577]
15. Li, Xiaojun X and 6 more authors.. (2009) Structural basis of double-stranded RNA recognition by the RIG-I like receptor MDA5.. Archives of biochemistry and biophysics, (1): [PMID:19531363]
16. Sato, Shinji S and 6 more authors.. (2009) RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial lung disease.. Arthritis and rheumatism, [PMID:19565506]
17. Pichlmair, Andreas A and 9 more authors.. (2009) Activation of MDA5 requires higher-order RNA structures generated during virus infection.. Journal of virology, [PMID:19656871]
18. You, Fuping F and 6 more authors.. (2009) PCBP2 mediates degradation of the adaptor MAVS via the HECT ubiquitin ligase AIP4.. Nature immunology, [PMID:19881509]
19. Nakashima, Ran R and 11 more authors.. (2010) The RIG-I-like receptor IFIH1/MDA5 is a dermatomyositis-specific autoantigen identified by the anti-CADM-140 antibody.. Rheumatology (Oxford, England), [PMID:20015976]
20. Oshiumi, Hiroyuki H, Sakai, Keisuke K, Matsumoto, Misako M and Seya, Tsukasa T.. (2010) DEAD/H BOX 3 (DDX3) helicase binds the RIG-I adaptor IPS-1 to up-regulate IFN-beta-inducing potential.. European journal of immunology, [PMID:20127681]
21. Chen, Rui R and 5 more authors.. (2010) The ubiquitin-specific protease 17 is involved in virus-triggered type I IFN signaling.. Cell research, [PMID:20368735]
22. Onoguchi, Kazuhide K, Yoneyama, Mitsutoshi M and Fujita, Takashi T.. (2011) Retinoic acid-inducible gene-I-like receptors.. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, [PMID:20950133]
23. Fu, Jihuan J, Xiong, Yanbao Y, Xu, Youli Y, Cheng, Genhong G and Tang, Hong H.. (2011) MDA5 is SUMOylated by PIAS2β in the upregulation of type I interferon signaling.. Molecular immunology, [PMID:21156324]
24. Loo, Yueh-Ming YM and Gale, Michael M.. (2011) Immune signaling by RIG-I-like receptors.. Immunity, (27): [PMID:21616437]
25. Miyashita, Moeko M, Oshiumi, Hiroyuki H, Matsumoto, Misako M and Seya, Tsukasa T.. (2011) DDX60, a DEXD/H box helicase, is a novel antiviral factor promoting RIG-I-like receptor-mediated signaling.. Molecular and cellular biology, [PMID:21791617]
26. Peisley, Alys A and 6 more authors.. (2011) Cooperative assembly and dynamic disassembly of MDA5 filaments for viral dsRNA recognition.. Proceedings of the National Academy of Sciences of the United States of America, (27): [PMID:22160685]
27. Xing, Junji J, Wang, Shuai S, Lin, Rongtuan R, Mossman, Karen L KL and Zheng, Chunfu C.. (2012) Herpes simplex virus 1 tegument protein US11 downmodulates the RLR signaling pathway via direct interaction with RIG-I and MDA-5.. Journal of virology, [PMID:22301138]
28. Menning, Maureen M and Kufer, Thomas A TA.. (2013) A role for the Ankyrin repeat containing protein Ankrd17 in Nod1- and Nod2-mediated inflammatory responses.. FEBS letters, (11): [PMID:23711367]
29. Feng, Qian Q and 10 more authors.. (2014) Enterovirus 2Apro targets MDA5 and MAVS in infected cells.. Journal of virology, [PMID:24390337]
30. Rice, Gillian I GI and 52 more authors.. (2014) Gain-of-function mutations in IFIH1 cause a spectrum of human disease phenotypes associated with upregulated type I interferon signaling.. Nature genetics, [PMID:24686847]
31. Oda, Hirotsugu H and 15 more authors.. (2014) Aicardi-Goutières syndrome is caused by IFIH1 mutations.. American journal of human genetics, (3): [PMID:24995871]
32. Rutsch, Frank F and 14 more authors.. (2015) A specific IFIH1 gain-of-function mutation causes Singleton-Merten syndrome.. American journal of human genetics, (5): [PMID:25620204]
33. Takashima, Ken K, Oshiumi, Hiroyuki H, Takaki, Hiromi H, Matsumoto, Misako M and Seya, Tsukasa T.. (2015) RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response.. Cell reports, (14): [PMID:25865883]
34. Wang, Shuai S and 7 more authors.. (2016) RNF123 has an E3 ligase-independent function in RIG-I-like receptor-mediated antiviral signaling.. EMBO reports, [PMID:27312109]
35. Rui, Yajuan Y and 12 more authors.. (2017) Disruption of MDA5-Mediated Innate Immune Responses by the 3C Proteins of Coxsackievirus A16, Coxsackievirus A6, and Enterovirus D68.. Journal of virology, (1): [PMID:28424289]
36. Meng, Jun J and 13 more authors.. (2017) ARRDC4 regulates enterovirus 71-induced innate immune response by promoting K63 polyubiquitination of MDA5 through TRIM65.. Cell death & disease, (8): [PMID:28594402]
37. Lamborn, Ian T IT and 25 more authors.. (2017) Recurrent rhinovirus infections in a child with inherited MDA5 deficiency.. The Journal of experimental medicine, (3): [PMID:28606988]
38. Zaki, Maha M and 6 more authors.. (2017) Recurrent and Prolonged Infections in a Child with a Homozygous IFIH1 Nonsense Mutation.. Frontiers in genetics, [PMID:29018476]
39. Lian, Huan H and 12 more authors.. (2018) The Zinc-Finger Protein ZCCHC3 Binds RNA and Facilitates Viral RNA Sensing and Activation of the RIG-I-like Receptors.. Immunity, (18): [PMID:30193849]
40. Li, Liang L and 5 more authors.. (2019) Encephalomyocarditis virus 2C protein antagonizes interferon-β signaling pathway through interaction with MDA5.. Antiviral research, [PMID:30312637]
41. Yin, Xin and 15 more authors.. (2021) MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells.. Cell reports, (12): [PMID:33440148]
42. Rebendenne, Antoine and 10 more authors.. (2021) SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells.. Journal of virology, (29): [PMID:33514628]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

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浓度 2 (C2)

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复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Recombinant MDA5 Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器