MAPRE1/EB1 Mouse mAb

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见COA

应用:

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse), 猪(Pig)
亚型: Mouse IgG2a
克隆号: C4
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab114244-10μl	10μl	现货
Ab114244-50μl	50μL	现货
Ab114244-100μl	100μL	现货
Ab114244-1ml	1ml	期货

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点击查看靶蛋白信息: MAPRE1

Accession#:Q15691 (1) EB1 (1) Gene ID:22919 (1) MAPRE1 (1)

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基本信息

产品名称	MAPRE1/EB1 Mouse mAb
别名	微管关联蛋白RP/EB家族成员1(MAPRE1)单克隆抗体 \| MAPRE1/EB1抗体 \| MAPRE1/EB1 小鼠单克隆抗体 \| MAPRE1/EB1 小鼠单抗
英文别名	EB1 \| 5530600P05Rik antibody \| Adenomatous polyposis coli binding protein EB 1 antibody \| Adenomatous polyposis coli binding protein EB1 antibody \| AI462499 antibody \| AI504412 antibody \| APC binding protein EB 1 antibody \| APC binding protein EB1 antibod
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属	小鼠(Mouse)
特异性	MAPRE1/EB1
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse),猪(Pig)
免疫原	Recombinant MAPRE1 expressed in E.coli (AA 2-268).
阳性对照	WB: C6, NIH/3T3, HEK293, Jurkat and HT-29 cell lysates. IHC: Human kidney tissue.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	C4
Format	Whole IgG
亚型	Mouse IgG2a
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A + Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂	0.05% Proclin 300
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

MAPRE1 Tbio 微管相关蛋白 RP/EB 家族成员 1(Microtubule-associated protein RP/EB family member 1) (0 活性数据)

点击查看靶蛋白信息： MAPRE1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: MAPRE1/EB1 Mouse mAb (Ab114244)

MAPRE1/EB1 Mouse mAb (Ab114244) - Western Blot
All lanes: MAPRE1/EB1 Mouse mAb (Ab114244) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 30.1 s

IHC: MAPRE1/EB1 Mouse mAb (Ab114244)

MAPRE1/EB1 Mouse mAb (Ab114244) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 10 µg/mL MAPRE1/EB1 Mouse mAb (Ab114244)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: MAPRE1/EB1 Mouse mAb (Ab114244)

MAPRE1/EB1 Mouse mAb (Ab114244) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 10 µg/mL MAPRE1/EB1 Mouse mAb (Ab114244)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

应用

应用名称	稀释比例
IHC	5-20 µg/mL
WB	0.01-3 µg/mL

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技术文档和文章

石蜡样品的免疫组化染色方案（IHC-P）

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单克隆抗体石蜡切片免疫组化染色

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重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

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Recombinant antibody Sodium Azide Removal Antibody Purification Sephadex G-25 Sodium Azide-Free Antibody

引用文献

1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J. (2001) The DNA sequence and comparative analysis of human chromosome 20.. Nature, 414 (6866): (865-71). [PMID:11780052]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
3. Su, L K LK and 8 more authors.. (1995) APC binds to the novel protein EB1.. Cancer research, (15): [PMID:7606712]
4. Berrueta, L L and 7 more authors.. (1998) The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:9724749]
5. Juwana, J P JP and 9 more authors.. (1999) EB/RP gene family encodes tubulin binding proteins.. International journal of cancer, (12): [PMID:10188731]
6. Berrueta, L L, Tirnauer, J S JS, Schuyler, S C SC, Pellman, D D and Bierer, B E BE.. (1999) The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain.. Current biology : CB, (22): [PMID:10226031]
7. Nakagawa, H H and 5 more authors.. (2000) EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue.. Oncogene, (13): [PMID:10644998]
8. Nakamura, Masafumi M, Zhou, X Z XZ, Kishi, Shuji S and Lu, K P KP.. (2002) Involvement of the telomeric protein Pin2/TRF1 in the regulation of the mitotic spindle.. FEBS letters, (13): [PMID:11943150]
9. Askham, J M JM, Vaughan, K T KT, Goodson, H V HV and Morrison, E E EE.. (2002) Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome.. Molecular biology of the cell, [PMID:12388762]
10. Hayashi, Ikuko I and Ikura, Mitsuhiko M.. (2003) Crystal structure of the amino-terminal microtubule-binding domain of end-binding protein 1 (EB1).. The Journal of biological chemistry, (19): [PMID:12857735]
11. Bu, Wen W and Su, Li-Kuo LK.. (2003) Characterization of functional domains of human EB1 family proteins.. The Journal of biological chemistry, (12): [PMID:14514668]
12. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843]
13. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455]
14. Honnappa, Srinivas S, John, Corinne M CM, Kostrewa, Dirk D, Winkler, Fritz K FK and Steinmetz, Michel O MO.. (2005) Structural insights into the EB1-APC interaction.. The EMBO journal, (26): [PMID:15616574]
15. Mimori-Kiyosue, Yuko Y and 10 more authors.. (2005) CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex.. The Journal of cell biology, (3): [PMID:15631994]
16. Mishima, Masaki M and 6 more authors.. (2007) Structural basis for tubulin recognition by cytoplasmic linker protein 170 and its autoinhibition.. Proceedings of the National Academy of Sciences of the United States of America, (19): [PMID:17563362]
17. Slep, Kevin C KC and Vale, Ronald D RD.. (2007) Structural basis of microtubule plus end tracking by XMAP215, CLIP-170, and EB1.. Molecular cell, (21): [PMID:17889670]
18. Komarova, Yulia Y and 12 more authors.. (2009) Mammalian end binding proteins control persistent microtubule growth.. The Journal of cell biology, (9): [PMID:19255245]
19. Jiang, Kai K and 7 more authors.. (2009) TIP150 interacts with and targets MCAK at the microtubule plus ends.. EMBO reports, [PMID:19543227]
20. Fong, Ka-Wing KW and 5 more authors.. (2009) Interaction of CDK5RAP2 with EB1 to track growing microtubule tips and to regulate microtubule dynamics.. Molecular biology of the cell, [PMID:19553473]
21. Honnappa, Srinivas S and 14 more authors.. (2009) An EB1-binding motif acts as a microtubule tip localization signal.. Cell, (23): [PMID:19632184]
22. Sahab, Ziad J ZJ and 6 more authors.. (2011) Tumor suppressor RARRES1 interacts with cytoplasmic carboxypeptidase AGBL2 to regulate the α-tubulin tyrosination cycle.. Cancer research, (15): [PMID:21303978]
23. van der Vaart, Babet B and 10 more authors.. (2011) SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase.. The Journal of cell biology, (13): [PMID:21646404]
24. Tanenbaum, Marvin E ME and 5 more authors.. (2011) A complex of Kif18b and MCAK promotes microtubule depolymerization and is negatively regulated by Aurora kinases.. Current biology : CB, (23): [PMID:21820309]
25. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
26. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]
27. Xia, Peng P and 10 more authors.. (2012) EB1 acetylation by P300/CBP-associated factor (PCAF) ensures accurate kinetochore-microtubule interactions in mitosis.. Proceedings of the National Academy of Sciences of the United States of America, (9): [PMID:23001180]
28. Wang, Xiwei X and 13 more authors.. (2012) Mitotic regulator SKAP forms a link between kinetochore core complex KMN and dynamic spindle microtubules.. The Journal of biological chemistry, (16): [PMID:23035123]
29. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
30. Zhu, Mei M and 7 more authors.. (2013) MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression.. The Journal of cell biology, (18): [PMID:23509069]
31. Lazarus, Jacob E JE, Moughamian, Armen J AJ, Tokito, Mariko K MK and Holzbaur, Erika L F EL.. (2013) Dynactin subunit p150(Glued) is a neuron-specific anti-catastrophe factor.. PLoS biology, [PMID:23874158]
32. Stroud, Matthew J MJ and 5 more authors.. (2014) GAS2-like proteins mediate communication between microtubules and actin through interactions with end-binding proteins.. Journal of cell science, (15): [PMID:24706950]
33. Mori, Yusuke Y and 6 more authors.. (2015) Cep169, a Novel Microtubule Plus-End-Tracking Centrosomal Protein, Binds to CDK5RAP2 and Regulates Microtubule Stability.. PloS one, [PMID:26485573]
34. Wei, Jieli J, Xu, Honglin H and Meng, Wenxiang W.. (2017) Noncentrosomal microtubules regulate autophagosome transport through CAMSAP2-EB1 cross-talk.. FEBS letters, [PMID:28726242]
35. Yang, Chao C and 10 more authors.. (2017) EB1 and EB3 regulate microtubule minus end organization and Golgi morphology.. The Journal of cell biology, (2): [PMID:28814570]
36. Bouguenina, Habib H and 10 more authors.. (2017) EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions.. Proceedings of the National Academy of Sciences of the United States of America, (12): [PMID:29162697]

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MAPRE1/EB1 Mouse mAb

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基本信息

产品属性

关联靶点（人）

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应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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引用文献

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