LysRS Antibody

COA

级别和纯度:

见COA

应用:

宿主种属: 兔(Rabbit)
种属反应性: 人(Human)
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab113848-10μl	10μl	现货
Ab113848-50μl	50μL	现货
Ab113848-100μl	100μL	现货
Ab113848-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: KARS

Accession#:Q15046 (2) Gene ID:3735 (2) KARS1 (2) LysRS (2)

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基本信息

产品名称	LysRS Antibody
别名	Anti-LysRS抗体 \| LysRS抗体 \| LysRS抗体
英文别名	CMTRIB \| DEAPLE \| DFNB89 \| KARS \| KARS2 \| KRS \| LEPID \| CMTRIB antibody \| DFNB89 antibody \| EC 6.1.1.6 antibody \| KARS 1 antibody \| KARS 2 antibody \| KARS antibody \| KARS1 antibody \| KARS2 antibody \| KIAA0070 antibody \| KRS antibody \| Lysine tRNA ligase a
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属	兔(Rabbit)
特异性	KARS1
种属反应性	人(Human)
免疫原	Recombinant KARS expressed in E.coli（AA 323-553）
阳性对照	WB: HEK293 and Hep G2 cell lysates. IHC: Human stomach, stomach cancer tissues.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂	0.05% Proclin 300
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

KARS Tchem 赖氨酸-tRNA连接酶(Lysine--tRNA ligase) (0 活性数据)

点击查看靶蛋白信息： KARS

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: LysRS Antibody (Ab113848)

LysRS Antibody (Ab113848) - Western Blot
All lanes: LysRS Antibody (Ab113848) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 68 kDa
Observed band size: 72 kDa
Exposure time: 174.6 s

IHC: LysRS Antibody (Ab113848)

LysRS Antibody (Ab113848) - IHC
DAB staining on IHC
Samples: Human Stomach Tissue
Primary Ab: 20 µg/mL LysRS Antibody (Ab113848)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: LysRS Antibody (Ab113848)

LysRS Antibody (Ab113848) - IHC
DAB staining on IHC
Samples: Human Stomach cancer Tissue
Primary Ab: 20 µg/mL LysRS Antibody (Ab113848)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: LysRS Antibody (Ab113848)

LysRS Antibody (Ab113848) - IHC
DAB staining on IHC
Sample: Human Stomach Tissue
Primary Ab: 20 µg/mL LysRS Antibody (Ab113848)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

应用

应用名称	稀释比例
IHC	5-20 µg/mL
WB	0.01-3 µg/mL

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0404088	分析证书	Ab113848
ZJ24F0404087	分析证书	Ab113848
ZJ24F0404086	分析证书	Ab113848

技术文档和文章

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

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重组抗体单克隆抗体 IHC 免疫组化抗原修复

免疫肽封闭实验

Categories: 实验方案（Protocols）

探索主题:

多克隆抗体免疫肽封闭特异性结合

Immunopeptide blocking assay

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Immunopeptideblocking PolyclonalAntibody SpecificBinding

Immunopeptide blocking assay

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Immunopeptideblocking PolyclonalAntibody SpecificBinding

叠氮化钠去除实验

Categories: 实验方案（Protocols）

探索主题:

重组抗体叠氮化钠去除抗体纯化葡聚糖凝胶G-25 无叠氮化钠抗体

Sodium Azide Removal Protocol

Categories: 实验方案（Protocols）

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Recombinant antibody Sodium Azide Removal Antibody Purification Sephadex G-25 Sodium Azide-Free Antibody

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引用文献

1. Galkin AV, Melnick JS, Kim S, Hood TL, Li N, Li L, Xia G, Steensma R, Chopiuk G, Jiang J et al.. (2007) Identification of NVP-TAE684, a potent, selective, and efficacious inhibitor of NPM-ALK.. Proc Natl Acad Sci USA, 104 (1): (270-5). [PMID:17185414]
2. Ballou LM, Selinger ES, Choi JY, Drueckhammer DG, Lin RZ. (2007) Inhibition of mammalian target of rapamycin signaling by 2-(morpholin-1-yl)pyrimido[2,1-alpha]isoquinolin-4-one.. J Biol Chem, 282 (33): (24463-70). [PMID:17562705]
3. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861]
4. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940]
5. Yin L, Li H, Liu W, Yao Z, Cheng Z, Zhang H, Zou H. (2018) A highly potent CDK4/6 inhibitor was rationally designed to overcome blood brain barrier in gliobastoma therapy.. Eur J Med Chem, 144 (1-28). [PMID:29247857]
6. Sophie Scheidecker,Séverine Bär,Corinne Stoetzel,Véronique Geoffroy,Béatrice Lannes,Bruno Rinaldi,Frédéric Fischer,Hubert D Becker,Valérie Pelletier,Cécile Pagan,Cécile Acquaviva-Bourdain,Stéphane Kremer,Marc Mirande,Christine Tranchant,Jean Muller,Sylvie Friant,Hélène Dollfus. (2019-05-23) Mutations in KARS cause a severe neurological and neurosensory disease with optic neuropathy.. Human mutation, 40 ((10)): (1826-1840). [PMID:31116475]
7. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
8. McDermott U, Sharma SV, Dowell L, Greninger P, Montagut C, Lamb J, Archibald H, Raudales R, Tam A, Lee D, Rothenberg SM, Supko JG, Sordella R, Ulkus LE, Iafrate AJ, Maheswaran S, Njauw CN, Tsao H, Drew L, Hanke JH, Ma XJ, Erlander MG, Gray NS, Haber DA, Settleman J.. (2007) Identification of genotype-correlated sensitivity to selective kinase inhibitors by using high-throughput tumor cell line profiling.. Proc Natl Acad Sci U S A, 104 (50): (19936-19941). [PMID:18077425] [10.1073/pnas.0707498104]
9. Hirabayashi A, Mukaiyama H, Kobayashi H, Shiohara H, Nakayama S, Ozawa M, Miyazawa K, Misawa K, Ohnota H, Isaji M.. (2008) A novel Syk family kinase inhibitor: design, synthesis, and structure-activity relationship of 1,2,4-triazolo[4,3-c]pyrimidine and 1,2,4-triazolo[1,5-c]pyrimidine derivatives.. Bioorg Med Chem, 16 (15): (7347-7357). [PMID:18585046] [10.1016/j.bmc.2008.06.017]
10. Hirabayashi A, Mukaiyama H, Kobayashi H, Shiohara H, Nakayama S, Ozawa M, Miyazawa K, Misawa K, Ohnota H, Isaji M.. (2009) Structure-activity relationship studies of 5-benzylaminoimidazo[1,2-c]pyrimidine-8-carboxamide derivatives as potent, highly selective ZAP-70 kinase inhibitors.. Bioorg Med Chem, 17 (1): (284-294). [PMID:19010686] [10.1016/j.bmc.2008.10.070]
11. Xia Z, Knaak C, Ma J, Beharry ZM, McInnes C, Wang W, Kraft AS, Smith CD.. (2009) Synthesis and evaluation of novel inhibitors of Pim-1 and Pim-2 protein kinases.. J Med Chem, 52 (1): (74-86). [PMID:19072652] [10.1021/jm800937p]
12. Yu H, Jung Y, Kim H, Lee J, Oh CH, Yoo KH, Sim T, Hah JM.. (2010) 1,4-dihydropyrazolo[4,3-d]imidazole phenyl derivatives: a novel type II Raf kinase inhibitors.. Bioorg Med Chem Lett, 20 (12): (3805-3808). [PMID:20466542] [10.1016/j.bmcl.2010.04.039]
13. Heidary DK, Huang G, Boucher D, Ma J, Forster C, Grey R, Xu J, Arnost M, Choquette D, Chen G, Zhou JH, Yao YM, Ball ED, Namchuk M, Davies RJ, Henkel G.. (2012) VX-322: a novel dual receptor tyrosine kinase inhibitor for the treatment of acute myelogenous leukemia.. J Med Chem, 55 (2): (725-734). [PMID:22221201] [10.1021/jm201198w]
14. Biamonte MA, Wanner J, Le Roch KG.. (2013) Recent advances in malaria drug discovery.. Bioorg Med Chem Lett, 23 (10): (2829-2843). [PMID:23587422] [10.1016/j.bmcl.2013.03.067]
15. He L, Li D, Zhang C, Bai P, Chen L.. (2017) Discovery of (R)-5-(benzo[d][1,3]dioxol-5-yl)-7-((1-(vinylsulfonyl)pyrrolidin-2-yl)methyl)-7H-pyrrolo[2,3-d]pyrimidin-4-amine (B6) as a potent Bmx inhibitor for the treatment of NSCLC.. Bioorg Med Chem Lett, 27 (17): (4171-4175). [PMID:28734581] [10.1016/j.bmcl.2017.07.009]
16. Malínková V, Řezníčková E, Jorda R, Gucký T, Kryštof V.. (2017) Trisubstituted purine inhibitors of PDGFRα and their antileukemic activity in the human eosinophilic cell line EOL-1.. Bioorg Med Chem, 25 (24): (6523-6535). [PMID:29089259] [10.1016/j.bmc.2017.10.032]
17. Li X, Huang Y, Cheng J, Zhang L, Mao F, Zhu J, Sheng C, Li J.. (2018) Discovery of novel Syk/PDGFR-α/c-Kit inhibitors as multi-targeting drugs to treat rheumatoid arthritis.. Bioorg Med Chem, 26 (15): (4375-4381). [PMID:30078608] [10.1016/j.bmc.2018.06.029]
18. Gao JB, Yang SJ, Yan ZR, Zhang XJ, Pu DB, Wang LX, Li XL, Zhang RH, Xiao WL.. (2018) Isolation, Characterization, and Structure-Activity Relationship Analysis of Abietane Diterpenoids from Callicarpa bodinieri as Spleen Tyrosine Kinase Inhibitors.. J Nat Prod, 81 (4): (998-1006). [PMID:29578342] [10.1021/acs.jnatprod.7b01082]
19. Ikegashira K, Ikenogami T, Yamasaki T, Oka T, Hase Y, Miyagawa N, Inagaki K, Kawahara I, Koga Y, Hashimoto H.. (2019) Optimization of an azetidine series as inhibitors of colony stimulating factor-1 receptor (CSF-1R) Type II to lead to the clinical candidate JTE-952.. Bioorg Med Chem Lett, 29 (7): (873-877). [PMID:30755337] [10.1016/j.bmcl.2019.02.006]
20. Nomura, N N and 9 more authors.. (1994) Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1.. DNA research : an international journal for rapid publication of reports on genes and genomes, [PMID:7584044]
21. Shiba, K K and 5 more authors.. (1997) Human lysyl-tRNA synthetase accepts nucleotide 73 variants and rescues Escherichia coli double-defective mutant.. The Journal of biological chemistry, (5): [PMID:9278442]
22. Quevillon, S S, Robinson, J C JC, Berthonneau, E E, Siatecka, M M and Mirande, M M.. (1999) Macromolecular assemblage of aminoacyl-tRNA synthetases: identification of protein-protein interactions and characterization of a core protein.. Journal of molecular biology, (8): [PMID:9878398]
23. Tolkunova, E E, Park, H H, Xia, J J, King, M P MP and Davidson, E E.. (2000) The human lysyl-tRNA synthetase gene encodes both the cytoplasmic and mitochondrial enzymes by means of an unusual alternative splicing of the primary transcript.. The Journal of biological chemistry, (10): [PMID:10952987]
24. Javanbakht, Hassan H and 6 more authors.. (2003) The interaction between HIV-1 Gag and human lysyl-tRNA synthetase during viral assembly.. The Journal of biological chemistry, (25): [PMID:12756246]
25. Lee, Yu-Nee YN, Nechushtan, Hovav H, Figov, Navah N and Razin, Ehud E.. (2004) The function of lysyl-tRNA synthetase and Ap4A as signaling regulators of MITF activity in FcepsilonRI-activated mast cells.. Immunity, [PMID:14975237]
26. Halwani, Rabih R and 6 more authors.. (2004) Cellular distribution of Lysyl-tRNA synthetase and its interaction with Gag during human immunodeficiency virus type 1 assembly.. Journal of virology, [PMID:15220430]
27. Martin, Joel J and 121 more authors.. (2004) The sequence and analysis of duplication-rich human chromosome 16.. Nature, (23): [PMID:15616553]
28. Park, Sang Gyu SG and 7 more authors.. (2005) Human lysyl-tRNA synthetase is secreted to trigger proinflammatory response.. Proceedings of the National Academy of Sciences of the United States of America, (3): [PMID:15851690]
29. Guzzo, Catherine M CM and Yang, David C H DC.. (2008) Lysyl-tRNA synthetase interacts with EF1alpha, aspartyl-tRNA synthetase and p38 in vitro.. Biochemical and biophysical research communications, (25): [PMID:18029264]
30. Guo, Min M, Ignatov, Michael M, Musier-Forsyth, Karin K, Schimmel, Paul P and Yang, Xiang-Lei XL.. (2008) Crystal structure of tetrameric form of human lysyl-tRNA synthetase: Implications for multisynthetase complex formation.. Proceedings of the National Academy of Sciences of the United States of America, (19): [PMID:18272479]
31. Kaminska, Monika M and 6 more authors.. (2009) Dissection of the structural organization of the aminoacyl-tRNA synthetase complex.. The Journal of biological chemistry, (6): [PMID:19131329]
32. Kaminska, Monika M and 5 more authors.. (2009) Dynamic Organization of Aminoacyl-tRNA Synthetase Complexes in the Cytoplasm of Human Cells.. The Journal of biological chemistry, (15): [PMID:19289464]
33. Yannay-Cohen, Nurit N and 8 more authors.. (2009) LysRS serves as a key signaling molecule in the immune response by regulating gene expression.. Molecular cell, (12): [PMID:19524539]
34. McLaughlin, Heather M HM and 24 more authors.. (2010) Compound heterozygosity for loss-of-function lysyl-tRNA synthetase mutations in a patient with peripheral neuropathy.. American journal of human genetics, (8): [PMID:20920668]
35. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
36. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]
37. Ofir-Birin, Yifat Y and 16 more authors.. (2013) Structural switch of lysyl-tRNA synthetase between translation and transcription.. Molecular cell, (10): [PMID:23159739]
38. Santos-Cortez, Regie Lyn P RL and 16 more authors.. (2013) Mutations in KARS, encoding lysyl-tRNA synthetase, cause autosomal-recessive nonsyndromic hearing impairment DFNB89.. American journal of human genetics, (11): [PMID:23768514]
39. Kim, Kyutae K and 7 more authors.. (2013) Reinvestigation of aminoacyl-tRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex.. PloS one, [PMID:24312579]
40. McMillan, Hugh J HJ and 10 more authors.. (2015) Congenital Visual Impairment and Progressive Microcephaly Due to Lysyl-Transfer Ribonucleic Acid (RNA) Synthetase (KARS) Mutations: The Expanding Phenotype of Aminoacyl-Transfer RNA Synthetase Mutations in Human Disease.. Journal of child neurology, [PMID:25330800]
41. Fang, Pengfei and 5 more authors.. (2015) Structural Basis for Specific Inhibition of tRNA Synthetase by an ATP Competitive Inhibitor.. Chemistry & biology, (18): [PMID:26074468]
42. Verrigni, D D and 12 more authors.. (2017) Novel mutations in KARS cause hypertrophic cardiomyopathy and combined mitochondrial respiratory chain defect.. Clinical genetics, [PMID:27891585]
43. Zhou, Xiao-Long XL and 6 more authors.. (2017) Mutations in KARS cause early-onset hearing loss and leukoencephalopathy: Potential pathogenic mechanism.. Human mutation, [PMID:28887846]
44. Ardissone, Anna A and 15 more authors.. (2018) KARS-related diseases: progressive leukoencephalopathy with brainstem and spinal cord calcifications as new phenotype and a review of literature.. Orphanet journal of rare diseases, (4): [PMID:29615062]
45. Ruzzenente, Benedetta B and 7 more authors.. (2018) Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.. Human mutation, [PMID:30252186]
46. Itoh, Masayuki M and 17 more authors.. (2019) Biallelic KARS pathogenic variants cause an early-onset progressive leukodystrophy.. Brain : a journal of neurology, (1): [PMID:30715177]
47. van der Knaap, Marjo S and 21 more authors.. (2019) Biallelic variants in LARS2 and KARS cause deafness and (ovario)leukodystrophy.. Neurology, (12): [PMID:30737337]

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稀释计算器

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浓度 2 (C2)

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复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

LysRS Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器