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Lamin B1 Mouse mAb

Lamin B1 Mouse mAb

级别和纯度:

见COA

应用:

IHC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 兔(Rabbit), 大鼠(Rat), 小鼠(Mouse), 山羊(Goat), 牛(Cow), 狗(Dog), 猪(Pig), 绵羊(Sheep), 豚鼠(Guinea pig)
亚型: Mouse IgG2b
克隆号: C2
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货	价格	数量
Ab112658-10μl	10μl	现货
Ab112658-50μl	50μL	现货
Ab112658-100μl	100μL	现货
Ab112658-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: LMNB1

Accession#:P20700 (7) Gene ID:4001 (7) Lamin B1 (7) LMNB1 (7) Loading control (34)

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基本信息

产品名称	Lamin B1 Mouse mAb
别名	核纤层蛋白B(内参)单克隆抗体 \| 核纤层蛋白B单克隆抗体 \| 核纤层蛋白B抗体 \| 层蛋白B1小鼠单克隆抗体 \| 层蛋白B1小鼠单抗
英文别名	ADLD antibody \| lamin B1 antibody \| Lamin-B1 antibody \| LMN antibody \| LMN2 antibody \| LMNB antibody \| Lmnb1 antibody \| LMNB1_HUMAN antibody \| MGC111419 antibody \| OTTHUMP00000159218 antibody \| ADLD \| Lamin B1 \| lamin-B1 \| LMN \| LMN2 \| LMNB \| LMNB1 \| MGC1
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属	小鼠(Mouse)
特异性	LMNB1
种属反应性	人(Human),兔(Rabbit),大鼠(Rat),小鼠(Mouse),山羊(Goat),牛(Cow),狗(Dog),猪(Pig),绵羊(Sheep),豚鼠(Guinea pig)
免疫原	Recombinant LMNB1 expressed in E.coli (AA 52-233).
阳性对照	WB: MCF7, HeLa and Raw264.7 cell lysates. IHC: Human Kidney Tissue, Human Stomach Tissue, Human Ovarian cancer Tissue, Human Thyroid cancer Tissue, Human amy.gdalitis Tissue and Human Lung cancer Tissue.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	C2
Format	Whole IgG
亚型	Mouse IgG2b
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A+G Affinity Chromatography
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4.
防腐剂	0.05% Proclin 300
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

LMNB1 Tbio 层蛋白B1(Lamin-B1) (0 活性数据)

点击查看靶蛋白信息： LMNB1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: Lamin B1 Mouse mAb (Ab112658)

Lamin B1 Mouse mAb (Ab112658) - Western Blot
All lanes: Lamin B1 Mouse mAb (Ab112658) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 66 kDa
Observed band size: 68 kDa
Exposure time: 30 s

IHC: Lamin B1 Mouse mAb (Ab112658)

Lamin B1 Mouse mAb (Ab112658) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658)
Second Ab: 2µg/mLHRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody .

IHC: Lamin B1 Mouse mAb (Ab112658)

Lamin B1 Mouse mAb (Ab112658) - IHC
DAB staining on IHC
Samples: Human Stomach Tissue
Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Lamin B1 Mouse mAb (Ab112658)

Lamin B1 Mouse mAb (Ab112658) - IHC
DAB staining on IHC
Samples: Human Ovarian cancer Tissue
Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Lamin B1 Mouse mAb (Ab112658)

Lamin B1 Mouse mAb (Ab112658) - IHC
DAB staining on IHC
Samples: Human Thyroid cancer Tissue
Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Lamin B1 Mouse mAb (Ab112658)

Lamin B1 Mouse mAb (Ab112658) - IHC
DAB staining on IHC
Samples: Human amy.gdalitis Tissue
Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Lamin B1 Mouse mAb (Ab112658)

Lamin B1 Mouse mAb (Ab112658) - IHC
DAB staining on IHC
Samples: Human Lung cancer Tissue
Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

应用

应用名称	稀释比例
IHC	5-20 µg/mL
WB	0.1-100 ng/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F0800962	分析证书	Ab112658
ZJ23F0800961	分析证书	Ab112658
ZJ24F0911225	分析证书	Ab112658

技术文档和文章

蛋白质印迹的上样对照

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一抗蛋白质印迹内参抗体

Loading Controls for Western Blotting

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Western blotting Primary antibody Loading control

Loading Controls for Western Blotting

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Western blotting Primary antibody Loading control

Loading Controls for Western Blotting

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Western blotting Primary antibody Loading control

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

叠氮化钠去除实验

Categories: 实验方案（Protocols）

探索主题:

重组抗体叠氮化钠去除抗体纯化葡聚糖凝胶G-25 无叠氮化钠抗体

Sodium Azide Removal Protocol

Categories: 实验方案（Protocols）

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Recombinant antibody Sodium Azide Removal Antibody Purification Sephadex G-25 Sodium Azide-Free Antibody

可替换产品

引用文献

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861]
2. Francis Impens,Lilliana Radoshevich,Pascale Cossart,David Ribet. (2014-08-13) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.. Proceedings of the National Academy of Sciences of the United States of America, 111 ((34)): (12432-12437). [PMID:25114211]
3. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
4. Pollard, K M KM and 5 more authors.. (1990) In vitro posttranslational modification of lamin B cloned from a human T-cell line.. Molecular and cellular biology, [PMID:2325650]
5. Lin, F F and Worman, H J HJ.. (1995) Structural organization of the human gene (LMNB1) encoding nuclear lamin B1.. Genomics, (20): [PMID:7557986]
6. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843]
7. Padiath, Quasar S QS and 8 more authors.. (2006) Lamin B1 duplications cause autosomal dominant leukodystrophy.. Nature genetics, [PMID:16951681]
8. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243]
9. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976]
10. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
11. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
12. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
13. Ruan, Jianbin J and 7 more authors.. (2012) Crystal structures of the coil 2B fragment and the globular tail domain of human lamin B1.. FEBS letters, (17): [PMID:22265972]
14. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
15. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315]
16. Johnson, Janel O JO and 41 more authors.. (2014) Mutations in the Matrin 3 gene cause familial amyotrophic lateral sclerosis.. Nature neuroscience, [PMID:24686783]
17. Yeh, Chung-Hsin CH and 8 more authors.. (2015) SEPT12/SPAG4/LAMINB1 complexes are required for maintaining the integrity of the nuclear envelope in postmeiotic male germ cells.. PloS one, [PMID:25775403]
18. Cristofoli, Francesca and 19 more authors.. (2020) De Novo Variants in LMNB1 Cause Pronounced Syndromic Microcephaly and Disruption of Nuclear Envelope Integrity.. American journal of human genetics, (1): [PMID:32910914]
19. Parry, David A and 17 more authors.. (2021) Heterozygous lamin B1 and lamin B2 variants cause primary microcephaly and define a novel laminopathy.. Genetics in medicine : official journal of the American College of Medical Genetics, [PMID:33033404]
20. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

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