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Recombinant Histone H3 Antibody

COA COA
    应用:
  • Flow Cytometry
  • IHC
  • IP
  • WB
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse), 斑马鱼(Zebrafish), 牛(Cow), 狗(Dog), 绿猴(Green Monkey)
  • 亚型: Rabbit IgG
  • 克隆号: RR692
  • 偶联: Unconjugated
有货

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab107980-10μl
 10μl 现货 Stock Image
Ab107980-50μl
 50μL 现货 Stock Image
Ab107980-100μl
 100μL 现货 Stock Image
大包装询价 收藏夹 比较  SDS下载  DATASHEET  质量标准
查看相关系列
点击查看靶蛋白信息: HIST1H3A
Accession#:P68431 (4) Gene ID:8350 (4) H3C1 (4) Histone H3 (4) 流式细胞术 (1163)
main product photo

基本信息

产品名称 Recombinant Histone H3 Antibody
别名 重组组蛋白H3抗体
英文别名 Histone H3/a | Histone H3/b | Histone H3/c | Histone H3/d | Histone H3/f | Histone H3/h | Histone H3/i | Histone H3/j | Histone H3/k | Histone H3/l
规格或纯度 ExactAb™, Validated, Recombinant, High performance, 1.0 mg/mL
宿主种属 兔(Rabbit)
特异性 H3C1
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse),斑马鱼(Zebrafish),牛(Cow),狗(Dog),绿猴(Green Monkey)
交叉反应 Cross-reactivity with other species has not been measured.
免疫原 A synthetic peptide corresponding to residues of human Histone 3.1 (AA 1-100).
阳性对照 WB: Hep G2, MOLT-4, A431, Jurkat, MCF7, HEK293, K562, THP-1, RAW 264.7 and C6 cell lysates. IHC: Human endometrial cancer tissue. Flow: HeLa cells. IP: HeLa whole cell lysate.
偶联 Unconjugated
备注 本产品即将售完下架，替代产品为 Ab213824

产品属性

克隆类型 重组抗体
克隆号 RR692
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3
防腐剂 0.01% Sodium azide
浓度 1.0 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点（人）

HIST1H3A Tbio 组蛋白 H3.1(Histone H3.1) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

Recombinant Histone H3 Antibody (Ab107980) - Western Blot
All lanes: Recombinant Histone H3 Antibody (Ab107980) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 15, 17 kDa
Exposure time: 7.1 s

Recombinant Histone H3 Antibody (Ab107980) - Western Blot
All lanes: Recombinant Histone H3 Antibody (Ab107980) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 15, 17 kDa
Exposure time: 33.2 s

Recombinant Histone H3 Antibody (Ab107980) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial cancer tissue labelling Histone H3 with Recombinant Histone H3 Antibody (Ab107980) at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Recombinant Histone H3 Antibody (Ab107980) - IP
Histone H3 was immunoprecipitated from 0.4 mg of HeLa whole cell lysate with Recombinant Histone H3 Antibody (Ab107980) at 100 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant Histone H3 Antibody (Ab107980) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant Histone H3 Antibody (Ab107980) in HeLa whole cell lysate
Lane 3: Hela whole cell lysate, 10 μg (input)

Recombinant Histone H3 Antibody (Ab107980) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant Histone H3 Antibody (Ab107980) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant Histone H3 Antibody (Ab107980) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

应用

应用名称 稀释比例
IHC

1/800 - 1/1600
Flow Cytometry

1/50 - 1/200
IHC

1/800 - 1/1600

IP

1/100
WB

1/1000 - 1/5000

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到5个结果

批号(Lot Number) 证书类型 货号
ZJ23F1201978 分析证书 Ab107980
ZJ23F1201979 分析证书 Ab107980
ZJ25F0319503 分析证书 Ab107980
ZJ24F1114499 分析证书 Ab107980
ZJ24F0202982 分析证书 Ab107980

技术文档和文章

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）
Categories: 实验方案（Protocols）
探索主题:
重组抗体 单克隆抗体 IHC 免疫组化 冷冻切片
免疫组织化学染色的抗原修复方案
Categories: 实验方案（Protocols）
探索主题:
重组抗体 单克隆抗体 IHC 免疫组化 抗原修复
组蛋白的蛋白质印迹法
Categories: 实验方案（Protocols）
探索主题:
免疫印迹 重组抗体 单克隆抗体 蛋白质印迹 HRP 辣根过氧化物酶 组蛋白
Histone Western Blot
Categories: 实验方案（Protocols）
探索主题:
Monoclonal antibodies Western blot Recombinant Antibodies HRP Immunoblotting Horseradish peroxidase Histone
免疫沉淀通用实验方案
Categories: 实验方案（Protocols）
探索主题:
免疫沉淀 蛋白质印迹 细胞裂解液 免疫共沉淀 IP 蛋白质互作 蛋白A/G磁珠
General Immunoprecipitation Protocol
Categories: 实验方案（Protocols）
探索主题:
Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads
General Immunoprecipitation Protocol
Categories: 实验方案（Protocols）
探索主题:
Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads
General Immunoprecipitation Protocol
Categories: 实验方案（Protocols）
探索主题:
Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads
General Immunoprecipitation Protocol
Categories: 实验方案（Protocols）
探索主题:
Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads
RNA免疫沉淀方案
Categories: 实验方案（Protocols）
探索主题:
交联 IP 蛋白A/G磁珠 RNA免疫沉淀 RIP RNA蛋白质互作 同种型对照抗体 Input
RNA Immunoprecipitation (RIP) Protocol
Categories: 实验方案（Protocols）
探索主题:
IP Protein A/G magnetic beads RIP Input RNA immunoprecipitation RNA protein interaction Isotype control antibody Crosslinking
RNA Immunoprecipitation (RIP) Protocol
Categories: 实验方案（Protocols）
探索主题:
IP Protein A/G magnetic beads RIP Input RNA immunoprecipitation RNA protein interaction Isotype control antibody Crosslinking
固定免疫沉淀实验方案
Categories: 实验方案（Protocols）
探索主题:
免疫沉淀 蛋白质印迹 WB IP 蛋白质互作 固定免疫沉淀 固定化抗体
Immobilized IP Protocol
Categories: 实验方案（Protocols）
探索主题:
Immunoprecipitation WB Western blot IP Protein interaction Immobilization immunoprecipitation Immobilized antibody
Immobilized IP Protocol
Categories: 实验方案（Protocols）
探索主题:
Immunoprecipitation WB Western blot IP Protein interaction Immobilization immunoprecipitation Immobilized antibody
利用甲醇通透法进行流式细胞术
Categories: 实验方案（Protocols）
探索主题:
流式细胞术 流式抗体 流式实验 甲醇通透 细胞分选 细胞分型 免疫研究 细胞内抗原检测
Flow Cytometry Using Methanol Permeabilization
Categories: 实验方案（Protocols）
探索主题:
Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection
Flow Cytometry Using Methanol Permeabilization
Categories: 实验方案（Protocols）
探索主题:
Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection
Western Blot
Categories: 技术文章
探索主题:
蛋白质印迹
Western Blot
Categories: 技术文章
探索主题:
Western blot

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引用文献

1. Ananthanarayanan M, Li S, Balasubramaniyan N, Suchy FJ, Walsh MJ.  (2004)  Ligand-dependent activation of the farnesoid X-receptor directs arginine methylation of histone H3 by CARM1..  J Biol Chem,  279  (52): (54348-57).  [PMID:15471871]
2. Marashi, F F and 6 more authors..  (1986)  Enhancer-facilitated expression of prokaryotic and eukaryotic genes using human histone gene 5' regulatory sequences..  Biochemistry and cell biology = Biochimie et biologie cellulaire,  [PMID:3013246]
3. Zhong, R R, Roeder, R G RG and Heintz, N N..  (1983)  The primary structure and expression of four cloned human histone genes..  Nucleic acids research,  (11): [PMID:6647026]
4. Ohe, Y Y and Iwai, K K..  (1981)  Human spleen histone H3. Isolation and amino acid sequence..  Journal of biochemistry,  [PMID:7309716]
5. Kardalinou, E E, Eick, S S, Albig, W W and Doenecke, D D..  (1993)  Association of a human H1 histone gene with an H2A pseudogene and genes encoding H2B.1 and H3.1 histones..  Journal of cellular biochemistry,  [PMID:8227173]
6. Goto, H H and 10 more authors..  (1999)  Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation..  The Journal of biological chemistry,  (3): [PMID:10464286]
7. Lachner, M M, O'Carroll, D D, Rea, S S, Mechtler, K K and Jenuwein, T T..  (2001)  Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins..  Nature,  (1): [PMID:11242053]
8. Goto, Hidemasa H, Yasui, Yoshihiro Y, Nigg, Erich A EA and Inagaki, Masaki M..  (2002)  Aurora-B phosphorylates Histone H3 at serine28 with regard to the mitotic chromosome condensation..  Genes to cells : devoted to molecular & cellular mechanisms,  [PMID:11856369]
9. Preuss, Ute U, Landsberg, Gerd G and Scheidtmann, Karl Heinz KH..  (2003)  Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase..  Nucleic acids research,  (1): [PMID:12560483]
10. Huyen, Yentram Y and 9 more authors..  (2004)  Methylated lysine 79 of histone H3 targets 53BP1 to DNA double-strand breaks..  Nature,  (18): [PMID:15525939]
11. Dai, Jun J, Sultan, Sammy S, Taylor, Stephen S SS and Higgins, Jonathan M G JM..  (2005)  The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment..  Genes & development,  (15): [PMID:15681610]
12. Choi, Hong Seok HS and 5 more authors..  (2005)  Phosphorylation of Ser28 in histone H3 mediated by mixed lineage kinase-like mitogen-activated protein triple kinase alpha..  The Journal of biological chemistry,  (8): [PMID:15684425]
13. Garcia, Benjamin A BA and 9 more authors..  (2005)  Modifications of human histone H3 variants during mitosis..  Biochemistry,  (4): [PMID:16185088]
14. Macdonald, Neil N and 12 more authors..  (2005)  Molecular basis for the recognition of phosphorylated and phosphoacetylated histone h3 by 14-3-3..  Molecular cell,  (28): [PMID:16246723]
15. Hake, Sandra B SB and 8 more authors..  (2006)  Expression patterns and post-translational modifications associated with mammalian histone H3 variants..  The Journal of biological chemistry,  (6): [PMID:16267050]
16. Flanagan, John F JF and 8 more authors..  (2005)  Double chromodomains cooperate to recognize the methylated histone H3 tail..  Nature,  (22): [PMID:16372014]
17. Thomas, C Eric CE, Kelleher, Neil L NL and Mizzen, Craig A CA..  (2006)  Mass spectrometric characterization of human histone H3: a bird's eye view..  Journal of proteome research,  [PMID:16457588]
18. Miao, Feng F, Li, ShuLian S, Chavez, Valerie V, Lanting, Linda L and Natarajan, Rama R..  (2006)  Coactivator-associated arginine methyltransferase-1 enhances nuclear factor-kappaB-mediated gene transcription through methylation of histone H3 at arginine 17..  Molecular endocrinology (Baltimore, Md.),  [PMID:16497732]
19. Beck, Hans Christian HC and 8 more authors..  (2006)  Quantitative proteomic analysis of post-translational modifications of human histones..  Molecular & cellular proteomics : MCP,  [PMID:16627869]
20. Morris, Stephanie A SA and 9 more authors..  (2007)  Identification of histone H3 lysine 36 acetylation as a highly conserved histone modification..  The Journal of biological chemistry,  (9): [PMID:17189264]
21. Garcia, Benjamin A BA and 10 more authors..  (2007)  Organismal differences in post-translational modifications in histones H3 and H4..  The Journal of biological chemistry,  (9): [PMID:17194708]
22. Guccione, Ernesto E and 7 more authors..  (2007)  Methylation of histone H3R2 by PRMT6 and H3K4 by an MLL complex are mutually exclusive..  Nature,  (18): [PMID:17898714]
23. Metzger, Eric E and 11 more authors..  (2008)  Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation..  Nature cell biology,  [PMID:18066052]
24. Iberg, Aimee N AN and 6 more authors..  (2008)  Arginine methylation of the histone H3 tail impedes effector binding..  The Journal of biological chemistry,  (8): [PMID:18077460]
25. Hyllus, Dawin D and 7 more authors..  (2007)  PRMT6-mediated methylation of R2 in histone H3 antagonizes H3 K4 trimethylation..  Genes & development,  (15): [PMID:18079182]
26. Shimada, Midori M and 6 more authors..  (2008)  Chk1 is a histone H3 threonine 11 kinase that regulates DNA damage-induced transcriptional repression..  Cell,  (25): [PMID:18243098]
27. Metzger, Eric E and 14 more authors..  (2010)  Phosphorylation of histone H3T6 by PKCbeta(I) controls demethylation at histone H3K4..  Nature,  (1): [PMID:20228790]
28. Vermeulen, Michiel M and 10 more authors..  (2010)  Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers..  Cell,  (17): [PMID:20850016]
29. Wu, Gang and 21 more authors..  (2012)  Somatic histone H3 alterations in pediatric diffuse intrinsic pontine gliomas and non-brainstem glioblastomas..  Nature genetics,  (29): [PMID:22286216]
30. Yu, Yongxin Y and 7 more authors..  (2012)  Histone H3 lysine 56 methylation regulates DNA replication through its interaction with PCNA..  Molecular cell,  (13): [PMID:22387026]
31. Yang, Weiwei W and 9 more authors..  (2012)  PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis..  Cell,  (17): [PMID:22901803]
32. Tropberger, Philipp P and 11 more authors..  (2013)  Regulation of transcription through acetylation of H3K122 on the lateral surface of the histone octamer..  Cell,  (14): [PMID:23415232]
33. Chan, Kui-Ming KM and 11 more authors..  (2013)  The histone H3.3K27M mutation in pediatric glioma reprograms H3K27 methylation and gene expression..  Genes & development,  (1): [PMID:23603901]
34. Lu, Chao C and 29 more authors..  (2016)  Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape..  Science (New York, N.Y.),  (13): [PMID:27174990]
35. Li, Lei L and 14 more authors..  (2016)  SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability..  Nature communications,  (20): [PMID:27436229]
36. Herranz, Nicolás N and 12 more authors..  (2016)  Lysyl oxidase-like 2 (LOXL2) oxidizes trimethylated lysine 4 in histone H3..  The FEBS journal,  [PMID:27735137]
37. Papillon-Cavanagh, Simon S and 22 more authors..  (2017)  Impaired H3K36 methylation defines a subset of head and neck squamous cell carcinomas..  Nature genetics,  [PMID:28067913]
38. Wang, Yugang Y and 17 more authors..  (2017)  KAT2A coupled with the α-KGDH complex acts as a histone H3 succinyltransferase..  Nature,  (14): [PMID:29211711]
39. Palazzo, Luca L and 5 more authors..  (2018)  Serine is the major residue for ADP-ribosylation upon DNA damage..  eLife,  (26): [PMID:29480802]

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