Western Blot

发布于 2025年8月26日更新时间 2025年8月26日

Sample Preparation

Protein Assay Kits and Pre-prepared Buffers – Fast, Simple, Efficient, and Cost-effective

The success of an experiment relies on high-quality sample preparation and precise, reliable quantitative analysis. To support your protein research, Aladdin has meticulously developed a series of reagents and kits tailored to critical steps of protein workflows. From accurate protein quantification to essential sample pre-treatment components such as reducing agents and pre-formulated buffers, these products comprehensively cover core experimental processes, seamlessly adapting to a variety of downstream applications and ensuring robust support throughout your experimental workflow.

Protein Assay Kits

Bradford Protein Assay Kit

BCA Protein Quantification Kit

Protein Quantification Kit

Reducing Agents

DL-Dithiothreitol (DTT)

β-Mercaptoethanol

Pre-prepared Sample Loading Buffers

Native PAGE Sample Buffer

Tricine SDS Loading Buffer

SDS-PAGE Loading Buffer

T751593

Name	Specification	ID
Protein Assay Kits
Bradford Protein Assay Kit	1000T	B774074
Bradford Assay Solution (Ready-to-use)	500ml	B406195
BCA Protein Assay Kit	500T	B665595
BCA Protein Quantitative Detection Kit (Ready to use)	500T	B736762
Wonderorangetm Protein Quantification Kit	100T/1000T	W598072
Reducing Agents
DL-Dithiothreitol	1g/5g/25g	D104861
2-Mercaptoethanol	25ml/100ml/250ml/1L/2.5L	M301574
Pre-prepared Sample Loading Buffers
UltraBio™ Blue Native PAGE Sample Buffer	1ml/5ml	B751548
Tricine SDS Loading Buffer (2X)	1ml/2×1ml/10×1ml	T751593
UltraBio™ SDS-PAGE Protein Staining and Loading Buffer	1ml	S751584

Electrophoresis

Pre-cast Gels – Safe, Fast, High Performance

Automated gel pouring ensures high stability and reproducibility of product quality.

Coated gel plates reduce protein adsorption, resulting in clearer bands.

Short electrophoresis time results in smooth and uniform bands.

Gel without SDS, suitable for both denaturing and non-denaturing electrophoresis.

Various concentrations of uniform and gradient gels are available.

Compatible with mainstream mini electrophoresis tanks on the market.

Gel Selection

Name	Gel Size	Gel Concentration	ID
UltraBio™ PAGE Precast Gel (Tris-Gly)	84×74×1mm	10%，12%，15%，4-15%，4-20%，8-16%，8-20%	E753596
UltraBio™ SDS-PAGE Precast Gel (Bis-Tris)	85×70×1mm	8%，19%，12%，4-12%，4-20%	U776776
UltraBio™ Blue Native Precast PAGE Gel	81×74×1.5mm	4-13%	B753588
UltraBio™ Plus Precast PAGE Gel(Hepes)	81×74×1.5mm	6%，8%，10%，12%，15%，4-15%，4-20%，8-20%	P753525
UltraBio™ Plus Precast PAGE Gel(Tris-Gly)	81×74×1.5mm	6%，8%，10%，12%，15%，4-15%，4-20%，8-20%	P753520
UltraBio™ Tricine Precast PAGE Gel for Small Molecular Weight Protein	81×74×1.5mm	16.5%	T753562
UltraBio™ Tris-Acetate Precast PAGE Gel for High Molecular Weight Protein	81×74×1.5mm	7%，3-8%	T753564

Electrophoresis

Protein Molecular Weight Markers – Accurate Quantification, Consistent Identification

rp192041

rp210256

Name	Number of Bands	Color	Specification	ID
Prestained Color Protein MW Marker (10-250kDa)	11	Blue, Orange, Green	250μL/4×250μl	rp192041
Prestained Color Protein MW Marker (25-300kDa)	9	Blue, Orange, Green	250μL/4×250μl	rp192284
Prestained Color Protein MW Marker (10-180kDa)	10	Blue, Orange, Green	250μL/4×250μl	rp192282
Prestained Color Protein MW Marker (2.7-40 kDa)	6	Blue, Orange, Green	250μL/4×250μl	rp192286
All Blue Protein MW Marker (10-250kDa)	12	Blue	250μL/4×250μl	rp210256

Transfer

Systems and Reagents – Fast Transfer, Efficient and Reliable

Transfer Buffer

Name	ID	Highlights
Western Rapid Transfer Buffer (20X, Wet)	T751592	Enables efficient protein transfer within 15–40 minutes
Western Rapid Transfer Buffer (10X, Wet & Semi-dry)	W743360	Suitable for both wet and semi-dry transfer methods
Western ice free bath rapid film transfer solution (10 ×）	W598073	Provides strong buffering capacity for complete transfer without ice bath requirement

PVDF Membranes & NC Membranes

Name	ID	Features
Polyvinylidene fluoride Membrane (PVDF)	P766358	Hydrophobic microporous membrane: high mechanical strength, excellent chemical resistance
Nitrocellulose Membrane (NC)	N766340	Hydrophilic microporous membrane: good protein-binding capacity

Immunodetection

Blocking, Chemiluminescence, and Fluorescence – Complete Blocking and Ultra-sensitive Detection

Blocking Solution

Rapid and efficient blocking of excess binding sites to reduce background signals.

High detection sensitivity and compatible with various antibody systems.

Name	Specification	ID
BSA Blocking Buffer	100ml/500ml	B670017
UltraBio™ WesternBlocking Buffer	100ml/500ml	W751598
UltraBio™ Western Blot Buffer Kit	4×100ml	W751600

Chemiluminescence

Ultra-high sensitivity

Strong and persistent signals

Enhanced chemiluminescent substrate

Suitable for various detection methods

Name	ID
ECL Plus	E266188
ECL Western Blot Kit	E665966

Internal control antibody

Western Blot - beta Tubulin Mouse mAb (Ab155823)

All lanes: beta Tubulin Mouse mAb at 1/10000 dilution

Samples: Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

Name	Grade & Purity	Packaging	ID
alpha Tubulin Mouse mAb	ExactAb™, Validated, Carrier Free, Azide Free, High performance, 0.5 mg/mL	1ml;100μl;50μl;10μl	Ab169013
beta Tubulin Mouse mAb	ExactAb™, Validated, Carrier Free, Azide Free, High performance, 0.8mg/mL	1ml;100μl;50μl;10μl	Ab155823
Recombinant Histone H3 Antibody	ExactAb™, Validated, Recombinant, High performance, 1.0 mg/mL	1ml;100μl;50μl;10μl	Ab107980

Tag Antibody

Name	Grade & Purity	Packaging	ID
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody	Animal-Free, Vector-Free, Recombinant, High Performance, Sodium Azide-Free, Verified, ExactAb™, ≥95%(SDS-PAGE), 1.0 mg/mL	1mg;100μg;50μg;10μg	Ab210603
Myc tag Mouse mAb	ExactAb™, Validated, High performance, 1.0 mg/mL	1ml;100μl; 50μl;10μl	Ab116621
GFP/EGFP Mouse mAb	ExactAb™, Validated, Carrier Free, Azide Free, ≥95%(SDS-PAGE), 1.0 mg/mL	1ml;100μl; 50μl;10μl	Ab169015

Primary Antibody

All lanes: AKT1 Mouse mAb (Ab087937) at 1/1000 dilution

Samples: Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution

Predicted band size: 55.7 kDa

Observed band size: 55.1 kDa

AKT1 Mouse mAb (Ab087937) - Western Blot

Name	Species Reactivity	Clonality	Grade & Purity	ID
AKT1 Mouse mAb	Human,Mouse,Rat,Monkey	Monoclonal	ExactAb™, Validated, Carrier Free, 1.0mg/mL	Ab087937
Recombinant ERK2 Antibody	Human,Mouse,Rat	Recombinant	ExactAb™, Validated, Recombinant, 0.5 mg/mL	Ab102233
Recombinant SHP1 Antibody	Human	Recombinant	ExactAb™, Validated, Recombinant, 0.3 mg/mL	Ab127501

Secondary Antibody

Name	Species Reactivity	Grade & Purity	ID
Goat Anti-Mouse IgG H&L (HRP)	Mouse	ExactAb™, High Performance, Verified, Sodium Azide-Free, 1.0 mg/mL	Ab179001
Goat Anti-Mouse IgG H&L (Biotin)	Mouse	ExactAb™, High Performance, Verified, Sodium Azide-Free, 1.0 mg/mL	Ab179002
Goat Anti-Human IgG Fc (FITC)	Human	ExactAb™, Verified, 1.0 mg/mL	Ab216758
Goat Anti-Human IgG (AF555)	Human	ExactAb™, Verified,Ex:553nm, Em:568nm, 1.0 mg/mL	Ab181961

Aladdin: https://www.aladdinsci.com/

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