货号 (SKU)

包装规格

是否现货

价格

数量

Ab105175-10μl

10μl

现货

Ab105175-50μl

50μL

现货

Ab105175-100μl

100μL

期货

Ab105175-1ml

1ml

期货

基本信息

产品名称	Gelsolin Mouse mAb
别名	凝溶胶蛋白(GSN)单克隆抗体 \| Gelsolin抗体 \| 凝胶素小鼠单克隆抗体 \| 凝胶素小鼠单抗
英文别名	Actin-depolymerizing factor \| ADF \| AGEL \| Brevin \| DKFZp313L0718 \| gelsolin (amyloidosis, Finnish type) \| Gelsolin \| GSN \| Actin depolymerizing factor antibody \| Actin-depolymerizing factor antibody \| ADF antibody \| AGEL antibody \| Brevin antibody \| DKFZ
规格或纯度	无载体, ExactAb™, 已验证, 高性能, 见COA
宿主种属	小鼠(Mouse)
特异性	Gelsolin
种属反应性	人(Human),大鼠(Rat),猪(Pig)
免疫原	Recombinant GSN expressed in E.coli (AA 434-782).
表位	The antibody is directed against an epitope located on the 47 kD peptide derived from a chymotryptic cleavage of gelsolin. This peptide contains the carboxy terminal actin binding site for gelsolin.
阳性对照	WB: Human serum, A549, HeLa and C6 lysates. IHC: Human kidney and colon tissues.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	D1
Format	Whole IgG
亚型	Mouse IgG1
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A + Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH 7.4
防腐剂	0.02% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

GSN Tbio 凝溶胶蛋白(Gelsolin) (0 活性数据)

点击查看靶蛋白信息： GSN

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Gelsolin Mouse mAb (Ab105175)

Gelsolin Mouse mAb (Ab105175) - Western Blot
All lanes: Gelsolin Mouse mAb (Ab105175) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 86 kDa
Observed band size: 90 kDa
Exposure time: 14.7 s

Western Blot: Gelsolin Mouse mAb (Ab105175)

Gelsolin Mouse mAb (Ab105175) - Western Blot
All lanes: Gelsolin Mouse mAb (Ab105175) at 1/1000 dilution
Samples: Lysates at 20 µL per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 86 kDa
Observed band size: 90 kDa
Exposure time: 10.7 s

IHC: Gelsolin Mouse mAb (Ab105175)

Gelsolin Mouse mAb (Ab105175) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL Gelsolin Mouse mAb (Ab105175)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Gelsolin Mouse mAb (Ab105175)

Gelsolin Mouse mAb (Ab105175) - IHC
DAB staining on IHC
Samples: Human Colon Tissue
Primary Ab: 10 µg/mL Gelsolin Mouse mAb (Ab105175)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

应用

应用名称	稀释比例
IHC	5-20 µg/mL
WB	0.01-3 µg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0303642	分析证书	Ab105175
ZJ24F0303641	分析证书	Ab105175
ZJ24F0303640	分析证书	Ab105175

技术文档和文章

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

引用文献

1. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940]
2. Nag S, Ma Q, Wang H, Chumnarnsilpa S, Lee WL, Larsson M, Kannan B, Hernandez-Valladares M, Burtnick LD, Robinson RC,. Ca2+ binding by domain 2 plays a critical role in the activation and stabilization of gelsolin.. Proc Natl Acad Sci U S A, [PMID:19666512]
3. de la Chapelle, A A and 6 more authors.. (1992) Gelsolin-derived familial amyloidosis caused by asparagine or tyrosine substitution for aspartic acid at residue 187.. Nature genetics, [PMID:1338910]
4. Maury, C P CP, Alli, K K and Baumann, M M.. (1990) Finnish hereditary amyloidosis. Amino acid sequence homology between the amyloid fibril protein and human plasma gelsoline.. FEBS letters, (15): [PMID:2153578]
5. Haltia, M M and 6 more authors.. (1990) Amyloid protein in familial amyloidosis (Finnish type) is homologous to gelsolin, an actin-binding protein.. Biochemical and biophysical research communications, (30): [PMID:2157434]
6. Ghiso, J J, Haltia, M M, Prelli, F F, Novello, J J and Frangione, B B.. (1990) Gelsolin variant (Asn-187) in familial amyloidosis, Finnish type.. The Biochemical journal, (15): [PMID:2176481]
7. Kwiatkowski, D J DJ and 5 more authors.. (1986) Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.. Nature, [PMID:3020431]
8. Lind, S E SE and Janmey, P A PA.. (1984) Human plasma gelsolin binds to fibronectin.. The Journal of biological chemistry, (10): [PMID:6092370]
9. McLaughlin, P J PJ, Gooch, J T JT, Mannherz, H G HG and Weeds, A G AG.. (1993) Structure of gelsolin segment 1-actin complex and the mechanism of filament severing.. Nature, (19): [PMID:8395021]
10. Xian, W W, Vegners, R R, Janmey, P A PA and Braunlin, W H WH.. (1995) Spectroscopic studies of a phosphoinositide-binding peptide from gelsolin: behavior in solutions of mixed solvent and anionic micelles.. Biophysical journal, [PMID:8599675]
11. Wen, D D and 5 more authors.. (1996) The plasma and cytoplasmic forms of human gelsolin differ in disulfide structure.. Biochemistry, (30): [PMID:8703941]
12. and Allen, P G PG.. (1997) Functional consequences of disulfide bond formation in gelsolin.. FEBS letters, (13): [PMID:9003812]
13. De Corte, V V and 5 more authors.. (1999) Identification of Tyr438 as the major in vitro c-Src phosphorylation site in human gelsolin: a mass spectrometric approach.. Protein science : a publication of the Protein Society, [PMID:10210201]
14. Choe, Han H and 5 more authors.. (2002) The calcium activation of gelsolin: insights from the 3A structure of the G4-G6/actin complex.. Journal of molecular biology, (6): [PMID:12460571]
15. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801]
16. Humphray, S J SJ and 147 more authors.. (2004) DNA sequence and analysis of human chromosome 9.. Nature, (27): [PMID:15164053]
17. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974]
18. Kim, Joon J and 8 more authors.. (2010) Functional genomic screen for modulators of ciliogenesis and cilium length.. Nature, (15): [PMID:20393563]
19. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
20. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

溶液计算器

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计算溶液所需的质量、体积或浓度。

质量

浓度

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g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

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体积 1 (V1)

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复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Gelsolin Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器

Gelsolin Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器