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CTNNA1 Antibody

    应用:
  • IF/ICC
  • IHC
  • WB
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human), 猪(Pig)
  • 亚型: Rabbit IgG
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab098106-10μl
10μl 现货 Stock Image
Ab098106-50μl
50μL 现货 Stock Image
Ab098106-100μl
100μL 现货 Stock Image
Ab098106-1ml
1ml 期货 Stock Image

基本信息

产品名称 CTNNA1 Antibody
别名 连环蛋白α1多克隆抗体 | CTNNA1抗体
英文别名 Alpha E-catenin antibody | Cadherin associated protein 102kDa antibody | Cadherin associated protein antibody | Cadherin-associated protein antibody | CAP 102 antibody | CAP102 antibody | Catenin (cadherin associated protein) alpha 1 102kDa antibody | Cat
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属 兔(Rabbit)
特异性 CTNNA1
种属反应性 人(Human),猪(Pig)
免疫原 Recombinant CTNNA1 expressed in E.coli (AA 2-228).
阳性对照 WB: HeLa and A31 lysates. IHC: Human kidney tissue. ICC/IF: HeLa cells.
偶联 Unconjugated

产品属性

克隆类型 多克隆抗体
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Antigen affinity purified + Protein A purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂 0.05% Proclin 300
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

CTNNA1 Tbio 连环蛋白α-1(Catenin alpha-1) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

CTNNA1 Antibody (Ab098106) - Western Blot
All lanes: CTNNA1 Antibody (Ab098106) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 100 kDa
Observed band size: 103 kDa
Exposure time: 58.4 s

CTNNA1 Antibody (Ab098106) - IF/ICC
FITC staining on IF
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CTNNA1 (green) using CTNNA1 Antibody (Ab098106) at 20μg/mL in ICC/IF.


CTNNA1 Antibody (Ab098106) - IHC
DAB staining on IHC
Samples: Human Stomach Tissue
Primary Ab: 10 µg/mL CTNNA1 Antibody (Ab098106)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

应用

应用名称 稀释比例
IHC

5-20 µg/mL

IF/ICC

5-20 µg/mL

WB

0.5-2µg/mL

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ23F1101698 分析证书 Ab098106
ZJ23F1101699 分析证书 Ab098106
ZJ24F1011937 分析证书 Ab098106

引用文献

1. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al..  (2017)  Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors..  Bioorg Med Chem,  25  (4): (1320-1328).  [PMID:28038940]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
3. Rimm, D L DL, Kebriaei, P P and Morrow, J S JS..  (1994)  Molecular cloning reveals alternative splice forms of human alpha(E)-catenin..  Biochemical and biophysical research communications,  (30): [PMID:7945318]
4. McPherson, J D JD and 7 more authors..  (1994)  Assignment of the human alpha-catenin gene (CTNNA1) to chromosome 5q21-q22..  Genomics,  (1): [PMID:8188230]
5. Oda, T T and 5 more authors..  (1993)  Cloning of the human alpha-catenin cDNA and its aberrant mRNA in a human cancer cell line..  Biochemical and biophysical research communications,  (30): [PMID:8323564]
6. Furukawa, Y Y and 6 more authors..  (1994)  Structure, expression and chromosome assignment of the human catenin (cadherin-associated protein) alpha 1 gene (CTNNA1)..  Cytogenetics and cell genetics,  [PMID:8404069]
7. Scanlan, M J MJ and 11 more authors..  (1999)  Antigens recognized by autologous antibody in patients with renal-cell carcinoma..  International journal of cancer,  (12): [PMID:10508479]
8. Marie, Helene H and 10 more authors..  (2003)  The LIM protein Ajuba is recruited to cadherin-dependent cell junctions through an association with alpha-catenin..  The Journal of biological chemistry,  (10): [PMID:12417594]
9. Schmutz, Jeremy J and 75 more authors..  (2004)  The DNA sequence and comparative analysis of human chromosome 5..  Nature,  (16): [PMID:15372022]
10. Gocke, Christian B CB, Yu, Hongtao H and Kang, Jungseog J..  (2005)  Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates..  The Journal of biological chemistry,  (11): [PMID:15561718]
11. Beranova-Giorgianni, Sarka S, Zhao, Yingxin Y, Desiderio, Dominic M DM and Giorgianni, Francesco F..  (2006)  Phosphoproteomic analysis of the human pituitary..  Pituitary,  [PMID:16807684]
12. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP..  (2006)  A probability-based approach for high-throughput protein phosphorylation analysis and site localization..  Nature biotechnology,  [PMID:16964243]
13. Abe, Kentaro K and Takeichi, Masatoshi M..  (2008)  EPLIN mediates linkage of the cadherin catenin complex to F-actin and stabilizes the circumferential actin belt..  Proceedings of the National Academy of Sciences of the United States of America,  (8): [PMID:18093941]
14. Han, Guanghui G and 9 more authors..  (2008)  Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography..  Proteomics,  [PMID:18318008]
15. Dephoure, Noah N and 6 more authors..  (2008)  A quantitative atlas of mitotic phosphorylation..  Proceedings of the National Academy of Sciences of the United States of America,  (5): [PMID:18669648]
16. Oppermann, Felix S FS and 7 more authors..  (2009)  Large-scale proteomics analysis of the human kinome..  Molecular & cellular proteomics : MCP,  [PMID:19369195]
17. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,  (18): [PMID:19690332]
18. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,  (15): [PMID:21406692]
19. Kask, Martin M, Pruunsild, Priit P and Timmusk, Tõnis T..  (2011)  Bidirectional transcription from human LRRTM2/CTNNA1 and LRRTM1/CTNNA2 gene loci leads to expression of N-terminally truncated CTNNA1 and CTNNA2 isoforms..  Biochemical and biophysical research communications,  (22): [PMID:21708131]
20. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,  (4): [PMID:23186163]
21. Majewski, Ian J IJ and 14 more authors..  (2013)  An α-E-catenin (CTNNA1) mutation in hereditary diffuse gastric cancer..  The Journal of pathology,  [PMID:23208944]
22. Hansford, Samantha S and 34 more authors..  (2015)  Hereditary Diffuse Gastric Cancer Syndrome: CDH1 Mutations and Beyond..  JAMA oncology,  [PMID:26182300]
23. Saksens, Nicole T M NT and 24 more authors..  (2016)  Mutations in CTNNA1 cause butterfly-shaped pigment dystrophy and perturbed retinal pigment epithelium integrity..  Nature genetics,  [PMID:26691986]
24. Caldwell, J M JM and 14 more authors..  (2017)  Cadherin 26 is an alpha integrin-binding epithelial receptor regulated during allergic inflammation..  Mucosal immunology,  [PMID:28051089]
25. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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