This is a demo store. No orders will be fulfilled.

首页   400-620-6333
  • 登录
  • 注册
  • 英文站
切换导航
我的购物车
搜索
高级搜索
菜单
登录
设置
྾

Recombinant COX IV Antibody

COA COA
    应用:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • IP
  • WB
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse), 牛(Cow), 狗(Dog), 猪(Pig), 绿猴(Green Monkey), 鸡(Chicken)
  • 亚型: Rabbit IgG
  • 克隆号: RR684
  • 偶联: Unconjugated
有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
Ab097410-10μl
 10μl 现货 Stock Image
Ab097410-50μl
 50μL 现货 Stock Image
Ab097410-100μl
 100μL 现货 Stock Image
Ab097410-1ml
 1ml 期货 Stock Image
大包装询价 收藏夹 比较  SDS下载  DATASHEET  质量标准
查看相关系列
点击查看靶蛋白信息: COX4I1
Accession#:P13073 (9) COX IV (7) COX4I1 (9) Gene ID:1327 (9) Loading control (34) 流式细胞术 (1163)
main product photo

基本信息

产品名称 Recombinant COX IV Antibody
别名 细胞色素c氧化酶IV(内参)单克隆抗体 | 细胞色素c氧化酶IV单克隆抗体 | 细胞色素c氧化酶IV抗体 | 重组 COX IV 抗体
英文别名 AL024441 antibody | COX 4 antibody | COX IV 1 antibody | COX IV antibody | COX IV-1 antibody | Cox4 antibody | COX41_HUMAN antibody | Cox4a antibody | COX4B antibody | COX4I1 antibody | COX4I2 antibody | COX4L2 antibody | COXIV antibody | Cytochrome c oxi
规格或纯度 ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL
宿主种属 兔(Rabbit)
特异性 COX4I1
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse),牛(Cow),狗(Dog),猪(Pig),绿猴(Green Monkey),鸡(Chicken)
免疫原 A synthetic peptide corresponding to residues on the N-terminus of human COX IV was used as an immunogen.
阳性对照 WB: A431, HEK293, HeLa, Jurkat, MCF7, A549, THP-1, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Liver tissue. Flow: HeLa cells. IF/ICC: HeLa cells. IP: HeLa whole cell lysate.
偶联 Unconjugated

产品属性

克隆类型 重组抗体
克隆号 RR684
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3
防腐剂 0.01% Sodium azide
浓度 0.5 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点（人）

COX4I1 Tbio 细胞色素 c 氧化酶亚基 4 同工酶 1，线粒体(Cytochrome c oxidase subunit 4 isoform 1, mitochondrial) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

Recombinant COX IV Antibody (Ab097410) - Western Blot
All lanes: Recombinant COX IV Antibody (Ab097410) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 17 kDa
Observed band size: 18 kDa
Exposure time: 15.1 s

Recombinant COX IV Antibody (Ab097410) - IF
IF analysis of COX IV (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Recombinant COX IV Antibody (Ab097410) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of liver tissue labelling COX IV with Recombinant COX IV Antibody (Ab097410) at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Recombinant COX IV Antibody (Ab097410) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant COX IV Antibody (Ab097410)(Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant COX IV Antibody (Ab097410) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

Recombinant COX IV Antibody (Ab097410) - IP
COX IV was immunoprecipitated from 0.4 mg of HeLa whole cell lysate with Recombinant COX IV Antibody (Ab097410) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant COX IV Antibody (Ab097410) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant COX IV Antibody (Ab097410) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input).

Exposure: 20.0 s

Recombinant COX IV Antibody (Ab097410) - IF
IF analysis of COX IV (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (TRITC) (Ab176448) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Recombinant COX IV Antibody (Ab097410) - IF
IF analysis of COX IV (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab176446) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

应用

应用名称 稀释比例
IP

1/50

WB

1/5000 – 1/10000

IHC

1/200 - 1/400

Flow Cytometry

1/200 - 1/1000

IF/ICC

1/10 - 1/500

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number) 证书类型 货号
ZJ23F0901105 分析证书 Ab097410
ZJ23F0901104 分析证书 Ab097410
ZJ24F0911325 分析证书 Ab097410

技术文档和文章

蛋白质印迹的上样对照
Categories: 技术文章
探索主题:
一抗 蛋白质印迹 内参抗体
Loading Controls for Western Blotting
Categories: 技术文章
探索主题:
Western blotting Primary antibody Loading control
流式细胞术
Categories: 技术文章
探索主题:
流式细胞术
流式细胞仪分析
Categories: 技术文章
探索主题:
前向散射 侧向散射 二向色滤光片 多色流式细胞仪
如何选择和使用抗体 — 一抗
Categories: 技术文章
探索主题:
重组蛋白 基因敲除 免疫细胞化学
How to choose and use antibodies-primary antibody?
Categories: 技术文章
探索主题:
Recombinant antibody Knockout gene ICC
抗体应用和技术
Categories: 技术文章
探索主题:
免疫印迹 免疫沉淀 酶联免疫吸附 免疫组织化学
Antibody applications and techniques
Categories: 技术文章
探索主题:
ICC ELISA WB IHC
Antibody applications and techniques
Categories: 技术文章
探索主题:
ICC ELISA WB IHC
流式细胞术实验方案
Categories: 实验方案（Protocols）
探索主题:
抗体 流式细胞术 流式抗体 活性染料 间接抗体标记
Loading Controls for Western Blotting
Categories: 技术文章
探索主题:
Western blotting Primary antibody Loading control
How to choose and use antibodies-primary antibody?
Categories: 技术文章
探索主题:
Recombinant antibody Knockout gene ICC
Antibody applications and techniques
Categories: 技术文章
探索主题:
ICC ELISA WB IHC
Antibody applications and techniques
Categories: 技术文章
探索主题:
ICC ELISA WB IHC
Loading Controls for Western Blotting
Categories: 技术文章
探索主题:
Western blotting Primary antibody Loading control
免疫沉淀(IP)裂解液和试剂
Categories: 实验方案（Protocols）
探索主题:
免疫沉淀 裂解液 组织裂解物
How to choose and use antibodies-primary antibody?
Categories: 技术文章
探索主题:
Recombinant antibody Knockout gene ICC
冷冻样本的免疫组织化学染色实验方案（IHC-Fr）
Categories: 实验方案（Protocols）
探索主题:
重组抗体 单克隆抗体 IHC 免疫组化 冷冻切片
免疫组织化学染色的抗原修复方案
Categories: 实验方案（Protocols）
探索主题:
重组抗体 单克隆抗体 IHC 免疫组化 抗原修复
免疫沉淀通用实验方案
Categories: 实验方案（Protocols）
探索主题:
免疫沉淀 蛋白质印迹 细胞裂解液 免疫共沉淀 IP 蛋白质互作 蛋白A/G磁珠
General Immunoprecipitation Protocol
Categories: 实验方案（Protocols）
探索主题:
Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads
General Immunoprecipitation Protocol
Categories: 实验方案（Protocols）
探索主题:
Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads
General Immunoprecipitation Protocol
Categories: 实验方案（Protocols）
探索主题:
Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads
General Immunoprecipitation Protocol
Categories: 实验方案（Protocols）
探索主题:
Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads
RNA免疫沉淀方案
Categories: 实验方案（Protocols）
探索主题:
交联 IP 蛋白A/G磁珠 RNA免疫沉淀 RIP RNA蛋白质互作 同种型对照抗体 Input
RNA Immunoprecipitation (RIP) Protocol
Categories: 实验方案（Protocols）
探索主题:
IP Protein A/G magnetic beads RIP Input RNA immunoprecipitation RNA protein interaction Isotype control antibody Crosslinking
RNA Immunoprecipitation (RIP) Protocol
Categories: 实验方案（Protocols）
探索主题:
IP Protein A/G magnetic beads RIP Input RNA immunoprecipitation RNA protein interaction Isotype control antibody Crosslinking
How to choose and use antibodies-primary antibody?
Categories: 技术文章
探索主题:
Recombinant antibody Knockout gene ICC
固定免疫沉淀实验方案
Categories: 实验方案（Protocols）
探索主题:
免疫沉淀 蛋白质印迹 WB IP 蛋白质互作 固定免疫沉淀 固定化抗体
Immobilized IP Protocol
Categories: 实验方案（Protocols）
探索主题:
Immunoprecipitation WB Western blot IP Protein interaction Immobilization immunoprecipitation Immobilized antibody
Immobilized IP Protocol
Categories: 实验方案（Protocols）
探索主题:
Immunoprecipitation WB Western blot IP Protein interaction Immobilization immunoprecipitation Immobilized antibody
利用甲醇通透法进行流式细胞术
Categories: 实验方案（Protocols）
探索主题:
流式细胞术 流式抗体 流式实验 甲醇通透 细胞分选 细胞分型 免疫研究 细胞内抗原检测
Flow Cytometry Using Methanol Permeabilization
Categories: 实验方案（Protocols）
探索主题:
Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection
Flow Cytometry Using Methanol Permeabilization
Categories: 实验方案（Protocols）
探索主题:
Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

相关产品

  1. Rabbit IgG-kappa (AF647), anti-HEL
    Rabbit IgG-kappa (AF647), anti-HEL

    ¥439.90

    收藏夹 比较
  2. Rabbit IgG (FITC)
    Rabbit IgG (FITC)

    ¥319.90

    收藏夹 比较
  3. Goat Anti-Rabbit IgG H&L (HRP)
    Goat Anti-Rabbit IgG H&L (HRP)

    ¥199.90

    收藏夹 比较
  4. Goat Anti-Rabbit IgG H&L Antibody
    Goat Anti-Rabbit IgG H&L Antibody

    ¥149.90

    收藏夹 比较
  5. Donkey Anti-Rabbit IgG H&L (AF647)
    Donkey Anti-Rabbit IgG H&L (AF647)

    ¥429.90

    收藏夹 比较
  6. Goat Anti-Rabbit IgG H&L (AF405)
    Goat Anti-Rabbit IgG H&L (AF405)

    ¥431.92

    收藏夹 比较
  7. Goat Anti-Rabbit IgG H&L (Biotin)
    Goat Anti-Rabbit IgG H&L (Biotin)

    ¥159.92

    收藏夹 比较
  8. Rabbit IgG-kappa (Biotin), anti-HEL
    Rabbit IgG-kappa (Biotin), anti-HEL

    ¥199.90

    收藏夹 比较
  9. Goat Anti-Rabbit IgG H&L (Cy5)
    Goat Anti-Rabbit IgG H&L (Cy5)

    ¥287.92

    收藏夹 比较
  10. Goat Anti-Rabbit IgG H&L (HRP)
    Goat Anti-Rabbit IgG H&L (HRP)

    ¥199.90

    收藏夹 比较
  11. Goat Anti-Rabbit IgG H&L (FITC)
    Goat Anti-Rabbit IgG H&L (FITC)

    ¥199.90

    收藏夹 比较
  12. Rabbit IgG
    Rabbit IgG

    ¥329.90

    收藏夹 比较
  13. Rabbit IgG-kappa (FITC), anti-HEL
    Rabbit IgG-kappa (FITC), anti-HEL

    ¥399.90

    收藏夹 比较
  14. Goat Anti-Rabbit IgG H&L Antibody
    Goat Anti-Rabbit IgG H&L Antibody

    ¥229.90

    收藏夹 比较
  15. Donkey Anti-Rabbit IgG H&L (Biotin)
    Donkey Anti-Rabbit IgG H&L (Biotin)

    ¥263.92

    收藏夹 比较
  16. Goat Anti-Rabbit IgG H&L (AF546)
    Goat Anti-Rabbit IgG H&L (AF546)

    ¥269.90

    收藏夹 比较
  17. Goat Anti-Rabbit IgG H&L (AF647)
    Goat Anti-Rabbit IgG H&L (AF647)

    ¥55.92

    收藏夹 比较
  18. Rabbit IgG-kappa, anti-HEL
    Rabbit IgG-kappa, anti-HEL

    ¥299.90

    收藏夹 比较
  19. Goat Anti-Rabbit IgG H&L (TRITC)
    Goat Anti-Rabbit IgG H&L (TRITC)

    ¥143.92

    收藏夹 比较
  20. Rabbit IgG (Biotin)
    Rabbit IgG (Biotin)

    ¥299.90

    收藏夹 比较

可替换产品

  1. Recombinant COX IV Antibody
    Recombinant COX IV Antibody

    ¥199.90

  2. COX IV Mouse mAb
    COX IV Mouse mAb

    ¥499.90

  3. COX IV Mouse mAb
    COX IV Mouse mAb

    ¥239.92

  4. COX IV Mouse mAb
    COX IV Mouse mAb

    ¥239.92

  5. COX IV Mouse mAb
    COX IV Mouse mAb

    ¥299.90

引用文献

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861]
2. Shuai Zong,Meng Wu,Jinke Gu,Tianya Liu,Runyu Guo,Maojun Yang.  (2018-07-22)  Structure of the intact 14-subunit human cytochrome c oxidase..  Cell research,  28  ((10)): (1026-1034).  [PMID:30030519]
3. Van Kuilenburg, A B AB and 5 more authors..  (1992)  Subunit IV of human cytochrome c oxidase, polymorphism and a putative isoform..  Biochimica et biophysica acta,  (26): [PMID:1311608]
4. Lomax, M I MI, Welch, M D MD, Darras, B T BT, Francke, U U and Grossman, L I LI..  (1990)  Novel use of a chimpanzee pseudogene for chromosomal mapping of human cytochrome c oxidase subunit IV..  Gene,  (14): [PMID:2157630]
5. Zeviani, M M and 8 more authors..  (1987)  Isolation of a cDNA clone encoding subunit IV of human cytochrome c oxidase..  Gene,  [PMID:2444497]
6. Bachman, N J NJ, Wu, W W, Schmidt, T R TR, Grossman, L I LI and Lomax, M I MI..  (1999)  The 5' region of the COX4 gene contains a novel overlapping gene, NOC4..  Mammalian genome : official journal of the International Mammalian Genome Society,  [PMID:10337626]
7. Bian, Yangyang Y and 9 more authors..  (2014)  An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome..  Journal of proteomics,  (16): [PMID:24275569]
8. Cesnekova, Jana J and 5 more authors..  (2016)  The mammalian homologue of yeast Afg1 ATPase (lactation elevated 1) mediates degradation of nuclear-encoded complex IV subunits..  The Biochemical journal,  (15): [PMID:26759378]
9. Abu-Libdeh, Bassam B and 7 more authors..  (2017)  Mutation in the COX4I1 gene is associated with short stature, poor weight gain and increased chromosomal breaks, simulating Fanconi anemia..  European journal of human genetics : EJHG,  [PMID:28766551]
10. Pillai, Nishitha R NR and 9 more authors..  (2019)  Biallelic variants in COX4I1 associated with a novel phenotype resembling Leigh syndrome with developmental regression, intellectual disability, and seizures..  American journal of medical genetics. Part A,  (10): [PMID:31290619]
11. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  5  (17-17).  [PMID:21269460]

溶液计算器

上海阿拉丁生化科技股份有限公司
© 2016 ALADDIN-E.COM . 版权所有,未经授权禁止拷贝本站资料,如有违反,将追究法律责任

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用。

上海工商 危险品化学品经营许可证（带存储）营业执照(三证合一)