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CD47 Mouse mAb

CD47 Mouse mAb

级别和纯度:

见COA

应用:

Flow Cytometry

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: CC2C6
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货	价格	数量
Ab094961-10μg	10μg	现货
Ab094961-50μg	50μg	现货
Ab094961-100μg	100μg	现货
Ab094961-1mg	1mg	期货

查看相关系列

点击查看靶蛋白信息: CD47

Accession#:Q08722 (16) Cd47 (19) Gene ID:961 (16) 流式细胞术 (1163)

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基本信息

产品名称	CD47 Mouse mAb
别名	整合素相关蛋白CD47 \| CD47抗体 \| Anti-CD47抗体 \| CD47小鼠单克隆抗体 \| CD47小鼠单抗
英文别名	antigen identified by monoclonal 1D8 \| Antigenic surface determinant protein OA3 \| CD47 antigen (Rh-related antigen, integrin-associated signal transducer) \| CD47 antigen \| CD47 glycoprotein \| CD47 molecule \| CD47 \| IAP \| IAPintegrin associated protein \|
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属	小鼠(Mouse)
特异性	CD47
种属反应性	人(Human)
免疫原	CCRF-CEM T-cell line.
阳性对照	Flow: Human peripheral blood lymphocytes.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	CC2C6
Format	Whole IgG
亚型	Mouse IgG1
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, pH 7.2-7.4
防腐剂	No
浓度	见COA
储存温度	2-8°C储存
运输条件	冰袋运输
稳定性与储存	4°C 下运输。4°C 长期保存（12 个月）。收货后建议分装。
分子类型	抗体

关联靶点（人）

CD47 Tchem 白细胞表面抗原 CD47(Leukocyte surface antigen CD47) (0 活性数据)

点击查看靶蛋白信息： CD47

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Flow Cytometry: CD47 Mouse mAb (Ab094961)

CD47 Mouse mAb (Ab094961) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD47 (red) with CD47 Mouse mAb (Ab094961) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD47 Mouse mAb (Ab094961)

CD47 Mouse mAb (Ab094961) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling CD47 (red) with CD47 Mouse mAb (Ab094961) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD47 Mouse mAb (Ab094961)

CD47 Mouse mAb (Ab094961) - Flow Cytometry
Flow Cytometry analysis of U937 cells labelling CD47 (red) with CD47 Mouse mAb (Ab094961) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD47 Mouse mAb (Ab094961)

CD47 Mouse mAb (Ab094961) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD47 with CD47 Mouse mAb (Ab094961) conjugated with biotin (right panel) at 2 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD47 Mouse mAb (Ab094961)

CD47 Mouse mAb (Ab094961) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD47 (red) with CD47 Mouse mAb (Ab094961) conjugated with biotin at 2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	1μg/100w cells

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0404667	分析证书	Ab094961
ZJ24F0404668	分析证书	Ab094961
ZJ24F0404669	分析证书	Ab094961

技术文档和文章

利用甲醇通透法进行流式细胞术

Categories: 实验方案（Protocols）

探索主题:

流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

可替换产品

引用文献

1. Kauder SE, Kuo TC, Harrabi O, Chen A, Sangalang E, Doyle L, Rocha SS, Bollini S, Han B, Sim J et al.. (2018) ALX148 blocks CD47 and enhances innate and adaptive antitumor immunity with a favorable safety profile.. PLoS One, 13 (8): (e0201832). [PMID:30133535]
2. Petrova PS, Viller NN, Wong M, Pang X, Lin GH, Dodge K, Chai V, Chen H, Lee V, House V et al.. (2017) TTI-621 (SIRPαFc): A CD47-Blocking Innate Immune Checkpoint Inhibitor with Broad Antitumor Activity and Minimal Erythrocyte Binding.. Clin Cancer Res, 23 (4): (1068-1079). [PMID:27856600]
3. Campbell, I G IG, Freemont, P S PS, Foulkes, W W and Trowsdale, J J.. (1992) An ovarian tumor marker with homology to vaccinia virus contains an IgV-like region and multiple transmembrane domains.. Cancer research, (1): [PMID:1394148]
4. Lindberg, F P FP, Gresham, H D HD, Schwarz, E E and Brown, E J EJ.. (1993) Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane-spanning domains implicated in alpha v beta 3-dependent ligand binding.. The Journal of cell biology, [PMID:7691831]
5. Mawby, W J WJ, Holmes, C H CH, Anstee, D J DJ, Spring, F A FA and Tanner, M J MJ.. (1994) Isolation and characterization of CD47 glycoprotein: a multispanning membrane protein which is the same as integrin-associated protein (IAP) and the ovarian tumour marker OA3.. The Biochemical journal, (1): [PMID:7998989]
6. Gao, A G AG and 5 more authors.. (1996) Integrin-associated protein is a receptor for the C-terminal domain of thrombospondin.. The Journal of biological chemistry, (5): [PMID:8550562]
7. Wu, A L AL, Wang, J J, Zheleznyak, A A and Brown, E J EJ.. (1999) Ubiquitin-related proteins regulate interaction of vimentin intermediate filaments with the plasma membrane.. Molecular cell, [PMID:10549293]
8. Rebres, R A RA, Vaz, L E LE, Green, J M JM and Brown, E J EJ.. (2001) Normal ligand binding and signaling by CD47 (integrin-associated protein) requires a long range disulfide bond between the extracellular and membrane-spanning domains.. The Journal of biological chemistry, (14): [PMID:11454874]
9. Latour, S S and 11 more authors.. (2001) Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:11509594]
10. Piccio, Laura L and 7 more authors.. (2005) Adhesion of human T cells to antigen-presenting cells through SIRPbeta2-CD47 interaction costimulates T-cell proliferation.. Blood, (15): [PMID:15383453]
11. Manna, Partha P PP, Dimitry, Julie J, Oldenborg, Per-Arne PA and Frazier, William A WA.. (2005) CD47 augments Fas/CD95-mediated apoptosis.. The Journal of biological chemistry, (19): [PMID:15917238]
12. Hatherley, Deborah D and 5 more authors.. (2008) Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47.. Molecular cell, (25): [PMID:18657508]
13. Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973]
14. De Oliveira, S S, Vitorino de Almeida, V V, Calado, A A, Rosário, H S HS and Saldanha, C C.. (2012) Integrin-associated protein (CD47) is a putative mediator for soluble fibrinogen interaction with human red blood cells membrane.. Biochimica et biophysica acta, [PMID:22079249]
15. Rogers, Natasha M NM and 24 more authors.. (2017) TSP1-CD47 signaling is upregulated in clinical pulmonary hypertension and contributes to pulmonary arterial vasculopathy and dysfunction.. Cardiovascular research, [PMID:27742621]
16. Ghimire, Kedar and 11 more authors.. (2020) CD47 Promotes Age-Associated Deterioration in Angiogenesis, Blood Flow and Glucose Homeostasis.. Cells, (15): [PMID:32679764]

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