CD45 Mouse mAb

COA

级别和纯度:

无载体

已验证

ExactAb™

见COA

应用:

Flow Cytometry

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: HI30
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab094902-10μg	10μg	现货
Ab094902-50μg	50μg	现货
Ab094902-100μg	100μg	期货
Ab094902-1mg	1mg	期货

查看相关系列

点击查看靶蛋白信息: PTPRC

Accession#:P08575 (57) Cd45 (70) Gene ID:5788 (53) Ptprc (73) 流式细胞术 (1163)

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基本信息

产品名称	CD45 Mouse mAb
别名	蛋白酪氨酸磷酸酶受体C(CD45)单克隆抗体 \| Anti-CD45抗体 \| CD45抗体 \| CD45单抗 \| CD45小鼠单克隆抗体 \| CD45小鼠单抗
英文别名	B220 \| CD_antigen: CD45 \| CD45 antigen \| CD45 \| CD45R \| EC 3.1.3.48 \| EC:3.1.3.48 \| GP180 \| LCA \| L-CA \| Leukocyte common antigen \| LY5 \| protein tyrosine phosphatase, receptor type, c polypeptide \| PTPRC \| receptor-type tyrosine-protein phosphatase C \| T
规格或纯度	无载体, ExactAb™, 已验证, 见COA
宿主种属	小鼠(Mouse)
特异性	CD45
种属反应性	人(Human)
免疫原	Peripheral blood mononuclear cells
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	HI30
Format	Whole IgG
亚型	Mouse IgG1
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 0.1% Sodium azide, pH 7.2
防腐剂	0.1% Sodium azide
浓度	见COA
储存温度	2-8°C储存
运输条件	冰袋运输
稳定性与储存	4°C 下运输。4°C 长期保存（36 个月）。收货后建议分装。
分子类型	抗体

关联靶点（人）

PTPRC Tchem 受体型酪氨酸蛋白磷酸酶C(Receptor-type tyrosine-protein phosphatase C) (0 活性数据)

点击查看靶蛋白信息： PTPRC

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Flow Cytometry: CD45 Mouse mAb (Ab094902)

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow analysis of CD45 (red line) in Raji cells. Cells were stained with CD45 Mouse mAb (Ab007182) at 0.1 μg/mL for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD45 Mouse mAb (Ab094902)

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow analysis of CD45 (red line) in Jurkat cells. Cells were stained with CD45 Mouse mAb (Ab007182) at 0.1 μg/mL for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD45 Mouse mAb (Ab094902)

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 with CD45 Mouse mAb (Ab094902) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD45 Mouse mAb (Ab094902)

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 (red) with CD45 Mouse mAb (Ab094902) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	< 0.5µg/100w cells

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0102526	分析证书	Ab094902
ZJ24F0102525	分析证书	Ab094902
ZJ24F0102524	分析证书	Ab094902

技术文档和文章

采用碘化丙啶DNA染色法进行流式细胞术细胞周期分析

Categories: 实验方案（Protocols）

探索主题:

流式抗体溴化丙锭细胞周期

流式实验样品制备实验指南

Categories: 实验方案（Protocols）

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蛋白样品制备流式细胞术流式抗体

Experimental Guide of Sample Preparation for Flow Cytometry

Categories: 实验方案（Protocols）

探索主题:

Protein sample preparation Flow Cytometry Antibody FACS

Experimental Guide of Sample Preparation for Flow Cytometry

Categories: 实验方案（Protocols）

探索主题:

Protein sample preparation Flow Cytometry Antibody FACS

利用甲醇通透法进行流式细胞术

Categories: 实验方案（Protocols）

探索主题:

流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

可替换产品

引用文献

1. Chen YT, Seto CT.. (2002) Divalent and trivalent alpha-ketocarboxylic acids as inhibitors of protein tyrosine phosphatases.. J Med Chem, 45 (18): (3946-3952). [PMID:12190316] [10.1021/jm020093q]
2. Andersen HS, Olsen OH, Iversen LF, Sørensen AL, Mortensen SB, Christensen MS, Branner S, Hansen TK, Lau JF, Jeppesen L, Moran EJ, Su J, Bakir F, Judge L, Shahbaz M, Collins T, Vo T, Newman MJ, Ripka WC, Møller NP.. (2002) Discovery and SAR of a novel selective and orally bioavailable nonpeptide classical competitive inhibitor class of protein-tyrosine phosphatase 1B.. J Med Chem, 45 (20): (4443-4459). [PMID:12238924] [10.1021/jm0209026]
3. He Y, Liu S, Menon A, Stanford S, Oppong E, Gunawan AM, Wu L, Wu DJ, Barrios AM, Bottini N et al.. (2013) A potent and selective small-molecule inhibitor for the lymphoid-specific tyrosine phosphatase (LYP), a target associated with autoimmune diseases.. J Med Chem, 56 (12): (4990-5008). [PMID:23713581]
4. Yu X, Sun JP, He Y, Guo X, Liu S, Zhou B, Hudmon A, Zhang ZY. (2007) Structure, inhibitor, and regulatory mechanism of Lyp, a lymphoid-specific tyrosine phosphatase implicated in autoimmune diseases.. Proc Natl Acad Sci USA, 104 (50): (19767-72). [PMID:18056643]
5. Stanford SM, Krishnamurthy D, Falk MD, Messina R, Debnath B, Li S, Liu T, Kazemi R, Dahl R, He Y et al.. (2011) Discovery of a novel series of inhibitors of lymphoid tyrosine phosphatase with activity in human T cells.. J Med Chem, 54 (6): (1640-54). [PMID:21341673]
6. Katherine A Rawls,P Therese Lang,Jun Takeuchi,Shinichi Imamura,Tyler D Baguley,Christoph Grundner,Tom Alber,Jonathan A Ellman. (2009-11-06) Fragment-based discovery of selective inhibitors of the Mycobacterium tuberculosis protein tyrosine phosphatase PtpA.. Bioorganic & medicinal chemistry letters, 19 ((24)): (6851-6854). [PMID:19889539]
7. Dufresne C, Roy P, Wang Z, Asante-Appiah E, Cromlish W, Boie Y, Forghani F, Desmarais S, Wang Q, Skorey K, Waddleton D, Ramachandran C, Kennedy BP, Xu L, Gordon R, Chan CC, Leblanc Y.. (2004) The development of potent non-peptidic PTP-1B inhibitors.. Bioorg Med Chem Lett, 14 (4): (1039-1042). [PMID:15013019] [10.1016/j.bmcl.2003.11.048]
8. Wilson DP, Wan ZK, Xu WX, Kirincich SJ, Follows BC, Joseph-McCarthy D, Foreman K, Moretto A, Wu J, Zhu M, Binnun E, Zhang YL, Tam M, Erbe DV, Tobin J, Xu X, Leung L, Shilling A, Tam SY, Mansour TS, Lee J.. (2007) Structure-based optimization of protein tyrosine phosphatase 1B inhibitors: from the active site to the second phosphotyrosine binding site.. J Med Chem, 50 (19): (4681-4698). [PMID:17705360] [10.1021/jm0702478]
9. Han Y, Belley M, Bayly CI, Colucci J, Dufresne C, Giroux A, Lau CK, Leblanc Y, McKay D, Therien M, Wilson MC, Skorey K, Chan CC, Scapin G, Kennedy BP.. (2008) Discovery of [(3-bromo-7-cyano-2-naphthyl)(difluoro)methyl]phosphonic acid, a potent and orally active small molecule PTP1B inhibitor.. Bioorg Med Chem Lett, 18 (11): (3200-3205). [PMID:18477508] [10.1016/j.bmcl.2008.04.064]
10. Cosconati S, Hong JA, Novellino E, Carroll KS, Goodsell DS, Olson AJ.. (2008) Structure-based virtual screening and biological evaluation of Mycobacterium tuberculosis adenosine 5'-phosphosulfate reductase inhibitors.. J Med Chem, 51 (21): (6627-6630). [PMID:18855373] [10.1021/jm800571m]
11. Yu WM, Guvench O, Mackerell AD, Qu CK.. (2008) Identification of small molecular weight inhibitors of Src homology 2 domain-containing tyrosine phosphatase 2 (SHP-2) via in silico database screening combined with experimental assay.. J Med Chem, 51 (23): (7396-7404). [PMID:19007293] [10.1021/jm800229d]
12. Vidović D, Schürer SC.. (2009) Knowledge-based characterization of similarity relationships in the human protein-tyrosine phosphatase family for rational inhibitor design.. J Med Chem, 52 (21): (6649-6659). [PMID:19810703] [10.1021/jm9008899]
13. Wu S, Vossius S, Rahmouni S, Miletic AV, Vang T, Vazquez-Rodriguez J, Cerignoli F, Arimura Y, Williams S, Hayes T, Moutschen M, Vasile S, Pellecchia M, Mustelin T, Tautz L.. (2009) Multidentate small-molecule inhibitors of vaccinia H1-related (VHR) phosphatase decrease proliferation of cervix cancer cells.. J Med Chem, 52 (21): (6716-6723). [PMID:19888758] [10.1021/jm901016k]
14. Zhang X, He Y, Liu S, Yu Z, Jiang ZX, Yang Z, Dong Y, Nabinger SC, Wu L, Gunawan AM, Wang L, Chan RJ, Zhang ZY.. (2010) Salicylic acid based small molecule inhibitor for the oncogenic Src homology-2 domain containing protein tyrosine phosphatase-2 (SHP2).. J Med Chem, 53 (6): (2482-2493). [PMID:20170098] [10.1021/jm901645u]
15. Vang T, Xie Y, Liu WH, Vidović D, Liu Y, Wu S, Smith DH, Rinderspacher A, Chung C, Gong G, Mustelin T, Landry DW, Rickert RC, Schürer SC, Deng SX, Tautz L.. (2011) Inhibition of lymphoid tyrosine phosphatase by benzofuran salicylic acids.. J Med Chem, 54 (2): (562-571). [PMID:21190368] [10.1021/jm101004d]
16. Yu ZH, Chen L, Wu L, Liu S, Wang L, Zhang ZY.. (2011) Small molecule inhibitors of SHP2 tyrosine phosphatase discovered by virtual screening.. Bioorg Med Chem Lett, 21 (14): (4238-4242). [PMID:21669525] [10.1016/j.bmcl.2011.05.078]
17. He Y, Xu J, Yu ZH, Gunawan AM, Wu L, Wang L, Zhang ZY.. (2013) Discovery and evaluation of novel inhibitors of mycobacterium protein tyrosine phosphatase B from the 6-Hydroxy-benzofuran-5-carboxylic acid scaffold.. J Med Chem, 56 (3): (832-842). [PMID:23305444] [10.1021/jm301781p]
18. Liu W, Yu B, Xu G, Xu WR, Loh ML, Tang LD, Qu CK.. (2013) Identification of cryptotanshinone as an inhibitor of oncogenic protein tyrosine phosphatase SHP2 (PTPN11).. J Med Chem, 56 (18): (7212-7221). [PMID:23957426] [10.1021/jm400474r]
19. Baguley TD, Xu HC, Chatterjee M, Nairn AC, Lombroso PJ, Ellman JA.. (2013) Substrate-based fragment identification for the development of selective, nonpeptidic inhibitors of striatal-enriched protein tyrosine phosphatase.. J Med Chem, 56 (19): (7636-7650). [PMID:24083656] [10.1021/jm401037h]
20. Tautz L, Senis YA, Oury C, Rahmouni S.. (2015) Perspective: Tyrosine phosphatases as novel targets for antiplatelet therapy.. Bioorg Med Chem, 23 (12): (2786-2797). [PMID:25921264] [10.1016/j.bmc.2015.03.075]
21. He R, Bai Y, Yu ZH, Wu L, Gunawan AM, Zhang ZY.. (2014) Diversity-Oriented Synthesis for Novel, Selective and Drug-like Inhibitors for a Phosphatase from Mycobacterium Tuberculosis.. Medchemcomm, 5 (10): (1496-1499). [PMID:25505942] [10.1039/c4md00099d]
22. Haftchenary S, Jouk AO, Aubry I, Lewis AM, Landry M, Ball DP, Shouksmith AE, Collins CV, Tremblay ML, Gunning PT.. (2015) Identification of Bidentate Salicylic Acid Inhibitors of PTP1B.. ACS Med Chem Lett, 6 (9): (982-986). [PMID:26396684] [10.1021/acsmedchemlett.5b00171]
23. Li Y, Xia G, Guo Q, Wu L, Chen S, Yang Z, Wang W, Zhang ZY, Zhou X, Jiang ZX.. (2016) Design, synthesis and evaluation of novel ¹⁹F magnetic resonance sensitive protein tyrosine phosphatase inhibitors.. Medchemcomm, 7 (8): (1672-1680). [PMID:27529021] [10.1039/C6MD00277C]
24. Abdjul DB, Yamazaki H, Kanno SI, Wewengkang DS, Rotinsulu H, Sumilat DA, Ukai K, Kapojos MM, Namikoshi M.. (2017) Furanoterpenes, new types of protein tyrosine phosphatase 1B inhibitors, from two Indonesian marine sponges, Ircinia and Spongia spp.. Bioorg Med Chem Lett, 27 (5): (1159-1161). [PMID:28189419] [10.1016/j.bmcl.2017.01.071]
25. He R,Wang J,Yu ZH,Zhang RY,Liu S,Wu L,Zhang ZY. (2016) Inhibition of Low Molecular Weight Protein Tyrosine Phosphatase by an Induced-Fit Mechanism.. J Med Chem, 59 (19.0): (9094-9106). [PMID:27676368] [10.1021/acs.jmedchem.6b00993]
26. Streuli, M M, Krueger, N X NX, Thai, T T, Tang, M M and Saito, H H.. (1990) Distinct functional roles of the two intracellular phosphatase like domains of the receptor-linked protein tyrosine phosphatases LCA and LAR.. The EMBO journal, [PMID:1695146]
27. Tsai, A Y AY, Streuli, M M and Saito, H H.. (1989) Integrity of the exon 6 sequence is essential for tissue-specific alternative splicing of human leukocyte common antigen pre-mRNA.. Molecular and cellular biology, [PMID:2531281]
28. Streuli, M M, Hall, L R LR, Saga, Y Y, Schlossman, S F SF and Saito, H H.. (1987) Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens.. The Journal of experimental medicine, (1): [PMID:2824653]
29. Charbonneau, H H, Tonks, N K NK, Walsh, K A KA and Fischer, E H EH.. (1988) The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2845400]
30. Ralph, S J SJ, Thomas, M L ML, Morton, C C CC and Trowbridge, I S IS.. (1987) Structural variants of human T200 glycoprotein (leukocyte-common antigen).. The EMBO journal, [PMID:2956090]
31. Hall, L R LR, Streuli, M M, Schlossman, S F SF and Saito, H H.. (1988) Complete exon-intron organization of the human leukocyte common antigen (CD45) gene.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:2971730]
32. Kung, C C and 12 more authors.. (2000) Mutations in the tyrosine phosphatase CD45 gene in a child with severe combined immunodeficiency disease.. Nature medicine, [PMID:10700239]
33. Jacobsen, M M and 15 more authors.. (2000) A point mutation in PTPRC is associated with the development of multiple sclerosis.. Nature genetics, [PMID:11101853]
34. Tchilian, E Z EZ and 5 more authors.. (2001) A deletion in the gene encoding the CD45 antigen in a patient with SCID.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:11145714]
35. Wu, Liangtang L, Fu, Jun J and Shen, Shi-Hsiang SH.. (2002) SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription.. Molecular and cellular biology, [PMID:11909961]
36. Fukuhara, Kenjiro K and 6 more authors.. (2002) A study on CD45 isoform expression during T-cell development and selection events in the human thymus.. Human immunology, [PMID:11975983]
37. Nam, Hyun-Joo HJ, Poy, Florence F, Saito, Haruo H and Frederick, Christin A CA.. (2005) Structural basis for the function and regulation of the receptor protein tyrosine phosphatase CD45.. The Journal of experimental medicine, (7): [PMID:15684325]
38. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414]
39. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974]
40. Panchal, Rekha G RG and 10 more authors.. (2009) Reduced expression of CD45 protein-tyrosine phosphatase provides protection against anthrax pathogenesis.. The Journal of biological chemistry, (8): [PMID:19269962]
41. Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973]
42. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
43. Gabaev, Ildar I and 9 more authors.. (2011) The human cytomegalovirus UL11 protein interacts with the receptor tyrosine phosphatase CD45, resulting in functional paralysis of T cells.. PLoS pathogens, [PMID:22174689]
44. Roberts, Joseph L JL and 12 more authors.. (2012) CD45-deficient severe combined immunodeficiency caused by uniparental disomy.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:22689986]
45. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
46. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

CD45 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器