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抗体
单克隆抗体
IL-33 Mouse mAb

IL-33 Mouse mAb

级别和纯度:

见COA

应用:

IHC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Mouse IgG2a
克隆号: C11
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货	价格	数量
Ab006917-10μl	10μl	现货
Ab006917-50μl	50μL	现货
Ab006917-100μl	100μL	现货
Ab006917-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: IL33

Accession#:O95760 (6) Gene ID:90865 (6) IL33 (7)

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main product photo

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基本信息

产品名称	IL-33 Mouse mAb
别名	IL-33 小鼠单克隆抗体 \| IL-33 小鼠单抗
英文别名	IL-1F11 \| IL-33 \| Interleukin-1 family member 11 \| NF-HEV \| Nuclear factor from high endothelial venules
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属	小鼠(Mouse)
特异性	IL33
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	Recombinant IL33 expressed in E.coli (AA 112-270).
阳性对照	WB: Jurkat, RAW 264.7, NIH/3T3 and C6 cell lysates. IHC: Human Stomach cancer Tissue, Human Prostate cancer Tissue, Human Colorectal cancer Tissue, Human Breast cancer Tissue, Human Liver Tissue.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	C11
Format	Whole IgG
亚型	Mouse IgG2a
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A + Protein G affinity chromatography
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂	0.05% Proclin 300
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
分子类型	抗体

关联靶点（人）

IL33 Tbio 白细胞介素-33(Interleukin-33) (0 活性数据)

点击查看靶蛋白信息： IL33

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - Western Blot
All lanes: IL-33 Mouse mAb (Ab006917) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 150.0 s

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Stomach cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Prostate cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IL-33 Mouse mAb (Ab006917)

IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

应用

应用名称	稀释比例
IHC	5-30 µg/mL
WB	0.01-3 µg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0102402	分析证书	Ab006917
ZJ24F0102401	分析证书	Ab006917
ZJ24F0102400	分析证书	Ab006917

技术文档和文章

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

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免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

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叠氮化钠去除实验

Categories: 实验方案（Protocols）

探索主题:

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Sodium Azide Removal Protocol

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探索主题:

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引用文献

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334]
2. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al.. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs.. Nat Genet, 36 (1): (40-5). [PMID:14702039]
3. Osbourn M, Soares DC, Vacca F, Cohen ES, Scott IC, Gregory WF, Smyth DJ, Toivakka M, Kemter AM, le Bihan T et al.. (2017) HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33.. Immunity, 47 (4): (739-751.e5). [PMID:29045903]
4. Onda, H H and 7 more authors.. (1999) Identification of genes differentially expressed in canine vasospastic cerebral arteries after subarachnoid hemorrhage.. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, [PMID:10566975]
5. Baekkevold, Espen S ES and 9 more authors.. (2003) Molecular characterization of NF-HEV, a nuclear factor preferentially expressed in human high endothelial venules.. The American journal of pathology, [PMID:12819012]
6. Humphray, S J SJ and 147 more authors.. (2004) DNA sequence and analysis of human chromosome 9.. Nature, (27): [PMID:15164053]
7. Schmitz, Jochen J and 12 more authors.. (2005) IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines.. Immunity, [PMID:16286016]
8. Carriere, Virginie V and 7 more authors.. (2007) IL-33, the IL-1-like cytokine ligand for ST2 receptor, is a chromatin-associated nuclear factor in vivo.. Proceedings of the National Academy of Sciences of the United States of America, (2): [PMID:17185418]
9. Komai-Koma, Mousa M and 5 more authors.. (2007) IL-33 is a chemoattractant for human Th2 cells.. European journal of immunology, [PMID:17853410]
10. Küchler, Axel M AM and 8 more authors.. (2008) Nuclear interleukin-33 is generally expressed in resting endothelium but rapidly lost upon angiogenic or proinflammatory activation.. The American journal of pathology, [PMID:18787100]
11. Moussion, Christine C, Ortega, Nathalie N and Girard, Jean-Philippe JP.. (2008) The IL-1-like cytokine IL-33 is constitutively expressed in the nucleus of endothelial cells and epithelial cells in vivo: a novel 'alarmin'?. PloS one, (6): [PMID:18836528]
12. Cayrol, Corinne C and Girard, Jean-Philippe JP.. (2009) The IL-1-like cytokine IL-33 is inactivated after maturation by caspase-1.. Proceedings of the National Academy of Sciences of the United States of America, (2): [PMID:19439663]
13. Talabot-Ayer, Dominique D, Lamacchia, Céline C, Gabay, Cem C and Palmer, Gaby G.. (2009) Interleukin-33 is biologically active independently of caspase-1 cleavage.. The Journal of biological chemistry, (17): [PMID:19465481]
14. Lüthi, Alexander U AU and 11 more authors.. (2009) Suppression of interleukin-33 bioactivity through proteolysis by apoptotic caspases.. Immunity, (17): [PMID:19559631]
15. Hayakawa, Morisada M and 5 more authors.. (2009) Mature interleukin-33 is produced by calpain-mediated cleavage in vivo.. Biochemical and biophysical research communications, (11): [PMID:19596270]
16. Lingel, Andreas and 7 more authors.. (2009) Structure of IL-33 and its interaction with the ST2 and IL-1RAcP receptors--insight into heterotrimeric IL-1 signaling complexes.. Structure (London, England : 1993), (14): [PMID:19836339]
17. Hong, Jaewoo J and 10 more authors.. (2011) Identification of constitutively active interleukin 33 (IL-33) splice variant.. The Journal of biological chemistry, (3): [PMID:21454686]
18. Ali, Shafaqat S and 6 more authors.. (2011) The dual function cytokine IL-33 interacts with the transcription factor NF-κB to dampen NF-κB-stimulated gene transcription.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:21734074]
19. Kakkar, Rahul R, Hei, Hillary H, Dobner, Stephan S and Lee, Richard T RT.. (2012) Interleukin 33 as a mechanically responsive cytokine secreted by living cells.. The Journal of biological chemistry, (24): [PMID:22215666]
20. Lefrançais, Emma E and 6 more authors.. (2012) IL-33 is processed into mature bioactive forms by neutrophil elastase and cathepsin G.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22307629]
21. Liu, Xi X and 7 more authors.. (2013) Structural insights into the interaction of IL-33 with its receptors.. Proceedings of the National Academy of Sciences of the United States of America, (10): [PMID:23980170]

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