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alpha-Smooth Muscle Actin Mouse mAb - Primary antibody, specific to alpha smooth muscle Actin, Mouse IgG2a

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse
  • Isotype: Mouse IgG2a
  • Clone Number: IJ07/4C7
  • Conjugation: Unconjugated
In stock
Item Number
Ab187009
Grouped product items
SKU Size
Availability
 Price Qty
Ab187009-10μl
10μl
10
$69.90
Ab187009-50μl
50μl
9
$199.90
Ab187009-100μl
100μl
4
$309.90
Ab187009-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (IJ07/4C7); Mouse anti human alpha-Smooth Muscle Actin; WB, Flow, ICC/IF, ELISA; Unconjugated

View related series
Click to view target information: ACTA2
Accession#:P62736 (7) ACTA2 (8) alpha-Smooth Muscle Actin (3) Flow Cytometry (1158) Gene ID:59 (5)

Basic Description

Product Name alpha-Smooth Muscle Actin Mouse mAb - Primary antibody, specific to alpha smooth muscle Actin, Mouse IgG2a
Synonyms AAT6 antibody | ACTA_HUMAN antibody | ACTA2 antibody | Actin alpha 2 smooth muscle aorta antibody | Actin aortic smooth muscle antibody | Actin, aortic smooth muscle antibody | ACTSA antibody | ACTVS antibody | a actin antibody | Alpha 2 actin antibody |
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE), 1.0 mg/mL
Host species Mouse
Specificity alpha smooth muscle Actin
Immunogen Recombinant full length protein corresponding to Human alpha smooth muscle Actin
Positive Control WB: HeLa and A431 whole cell lysates; Mouse skeletal muscle and Mouse heart tissue lysates Flow: HeLa and Jurkat cells ICC/IF: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

WB: 1/1,000 - 1/20,000; Predicted molecular weight: 42.0 KDa

Flow: 1/200 - 1/50,000

ICC/IF: 1/1,000

Product Properties

Isotype Mouse IgG2a
Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

ACTA2 Tbio Actin, aortic smooth muscle (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

alpha-Smooth Muscle Actin Mouse mAb (Ab187009) - Western Blot
All lanes: alpha-Smooth Muscle Actin Mouse Antibody (Ab187009) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 43 kDa
Exposure time: 60.0 s

alpha-Smooth Muscle Actin Mouse mAb (Ab187009) - Western Blot
All lanes: alpha-Smooth Muscle Actin Mouse Antibody (Ab187009) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 54.9 s

alpha-Smooth Muscle Actin Mouse mAb (Ab187009) - Western Blot
All lanes: alpha-Smooth Muscle Actin Mouse Antibody (Ab187009) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 60.0 s

alpha-Smooth Muscle Actin Mouse mAb (Ab187009) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with alpha-Smooth Muscle Actin Mouse mAb (Ab187009) (Red) at 0.2 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

alpha-Smooth Muscle Actin Mouse mAb (Ab187009) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells stained with alpha-Smooth Muscle Actin Mouse mAb (Ab187009) (Red) at 0.2 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

alpha-Smooth Muscle Actin Mouse mAb (Ab187009) - ICC/IF
IF analysis of alpha-Smooth Muscle Actin (Red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with alpha-Smooth Muscle Actin Mouse mAb (Ab187009) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
Flow Cytometry

1/200 - 1/50,000
WB

1/1,000 - 1/20,000; Predicted molecular weight: 42.0 KDa
IF/ICC

1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0708535 Certificate of Analysis Jul 19, 2024 Ab187009
ZJ24F0708536 Certificate of Analysis Jul 19, 2024 Ab187009
ZJ24F0708537 Certificate of Analysis Jul 19, 2024 Ab187009

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