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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab125269-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$69.90
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Ab125269-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$289.90
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Ab125269-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$309.90
|
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Ab125269-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
|
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Recombinant; Rabbit anti Human RhoA Antibody; WB, IF/ICC; Unconjugated
| Product Name | Recombinant RhoA Antibody - Primary antibody, specific to RHOA, Rabbit IgG |
|---|---|
| Synonyms | EC 3.6.5.2 | h12 | Rho cDNA clone 12 | ARH12 | ARHA | H12 | RHO12 | RHOA | RHOB | RHOC | RHOH12 | Small GTP binding protein RhoA | Transforming protein RhoA | Aplysia ras related homolog 12 antibody | ARH12 antibody | ARHA antibody | H 12 antibody | H12 a |
| Specifications & Purity | Recombinant, ExactAb™, Validated, See COA |
| Biochemical and Physiological Mechanisms | Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotubercu |
| Host species | Rabbit |
| Specificity | RHOA |
| Immunogen | A synthetic peptide derived from human RhoA (AA 150-193). |
| Positive Control | WB: HeLa, Jurkat, Hep G2, MCF7, HUVEC, HEK293, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa, Jurkat cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human RhoA Antibody, Recombinant, could be used for WB, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
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| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
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Recombinant RhoA Antibody (Ab125269) - Western Blot
All lanes: Recombinant RhoA Antibody (Ab125269) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 100.0 s
Recombinant RhoA Antibody (Ab125269) - IF/ICC
Immunofluorescent analysis of Jurkat cells, using Recombinant RhoA Antibody (Ab125269).
Recombinant RhoA Antibody (Ab125269) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant RhoA Antibody (Ab125269) at 1/50 dilution.
| Application | Dilution info |
|---|---|
| WB | 1/1000 - 1/2000 |
| IF/ICC | 1/50 - 1/200 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Feb 19, 2025 | Ab125269 |