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Recombinant p27 KIP 1 Antibody - Primary antibody, specific to p27 KIP 1, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab119643
Grouped product items
SKU Size
Availability
Price Qty
Ab119643-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab119643-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$209.90
Ab119643-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$349.90
Ab119643-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

Recombinant; Rabbit anti Human p27 KIP 1 Antibody; WB, IHC, ICC, IF; Unconjugated

Basic Description

Product Name Recombinant p27 KIP 1 Antibody - Primary antibody, specific to p27 KIP 1, Rabbit IgG
Synonyms AI843786 antibody | Cdki1b antibody | CDKN 1B antibody | CDKN 4 antibody | CDKN1B antibody | CDKN4 antibody | Cyclin Dependent Kinase Inhibitor 1B antibody | Cyclin dependent kinase inhibitor p27 antibody | AA408329 antibody | Cyclin-dependent kinase inhi
Specifications & Purity Recombinant, ExactAb™, KD Validation, Validated, See COA
Host species Rabbit
Specificity p27 KIP 1
Immunogen A synthetic peptide derived from human p27 KIP 1 (AA 150-198).
Positive Control WB: HeLa, A549, MCF7, Jurkat, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human colon tissue. IF/ICC: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, KD Validation, Recombinant, Validated
Product Description

Rabbit anti Human p27 KIP 1 Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on.
Application
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
Protein Function
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

CDKN1B Tbio Cyclin-dependent kinase inhibitor 1B (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant p27 KIP 1 Antibody (Ab119643) - Western Blot
All lanes: Recombinant p27 KIP 1 Antibody (Ab119643) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 22 kDa
Observed band size: 27 kDa
Exposure time: 80.0 s

Recombinant p27 KIP 1 Antibody (Ab119643) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant p27 KIP 1 Antibody (Ab119643).

Recombinant p27 KIP 1 Antibody (Ab119643) - IHC
Immunohistochemical analysis of paraffin-embedded human colon, using Recombinant p27 KIP 1 Antibody (Ab119643).

Application

Application Dilution info
IF/ICC

1/50-1/200

IHC

1/50-1/200

WB

1/500-1/2000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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