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Recombinant NELFe Antibody - Primary antibody, specific to NELFE, Rabbit IgG

    Application:
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab117537
Grouped product items
SKU Size
Availability
Price Qty
Ab117537-10μl
10μl
2
$89.90
Ab117537-50μl
50μl
1
$269.90
Ab117537-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$379.90
Ab117537-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Recombinant; Rabbit anti Human NELFe Antibody; WB, IHC; Unconjugated

Basic Description

Product Name Recombinant NELFe Antibody - Primary antibody, specific to NELFE, Rabbit IgG
Synonyms Major histocompatibility complex gene RD | Negative elongation factor E | Negative elongation factor polypeptide E | NELF e | NELF-E | Nelfe | NELFE_HUMAN | Nuclear protein | RD | RD protein | RDBP | RD RNA binding protein | RDP | RNA-binding protein RD |
Specifications & Purity ExactAb™, Validated, Recombinant, 0.3 mg/mL
Host species Rabbit
Specificity NELFE
Immunogen Recombinant protein of human NELFe (AA 1-150)
Positive Control WB: HeLa and NIH/3T3 cell lysates. IHC: Human breast cancer tissue.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human NELFe Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/2000
IHC: 1/50-1/100
Protein Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Antigen affinity purified
Shape Liquid
Concentration 0.3 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

GBP1 Tbio Guanylate-binding protein 1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
NELFE Tbio Negative elongation factor E (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant NELFe Antibody (Ab117537) - Western Blot
All lanes: Recombinant NELFe Antibody (Ab117537) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 45, 47 kDa
Exposure time: 2.3 s 

Recombinant NELFe Antibody (Ab117537) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant NELFe Antibody (Ab117537). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/2000

IHC

1/50 - 1/100

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0606436 Certificate of Analysis Jun 03, 2024 Ab117537
ZJ24F0606435 Certificate of Analysis Jun 03, 2024 Ab117537

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