Grouped product items

SKU

Size

Availability

Price

Qty

Ab116092-10μl

10μl

$69.90

Ab116092-50μl

50μl

$199.90

Ab116092-100μl

100μl

Available within 8-12 weeks(?)

$319.90

Ab116092-1ml

1ml

Available within 8-12 weeks(?)

$1,999.90

Basic Description

Product Name	Recombinant MSH2 Antibody - Primary antibody, specific to MSH2, Rabbit IgG
Synonyms	MutS protein homolog 2 \| mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) \| MSH2 \| LCFS2 \| COCA 1 antibody \| BAT26 antibody \| COCA1 antibody \| DNA mismatch repair protein Msh2 antibody \| FCC 1 antibody \| HNPCC1mutS (E. coli) homolog 2 (colon ca
Specifications & Purity	ExactAb™, Validated, Recombinant, 1.2 mg/mL
Host species	Rabbit
Specificity	MSH2
Immunogen	Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).
Positive Control	IHC: Human colorectal cancer tissue.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human MSH2 Antibody, Recombinant (MyR1-MSH2), could be used for IHC and so on. Application IHC: 1/100-1/200 Protein Function Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	1.2 mg/mL
Storage Temp	Store at 2-8°C,Avoid repeated freezing and thawing
Shipped In	Wet ice
Stability And Storage	Store at 2-8°C long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MSH2 Tbio DNA mismatch repair protein Msh2 (0 Activities)

Discover Target: MSH2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

IHC: Recombinant MSH2 Antibody (Ab116092)

Recombinant MSH2 Antibody (Ab116092) - IHC
Immunohistochemistry analysis of paraffin-embedded human intestinal cancer tissue labelling MSH2 with Recombinant MSH2 Antibody (Ab116092) at 1/100 dilution. The section was incubated with Recombinant MSH2 Antibody (Ab116092) for 30 mins at room temperature. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was used for antigen retrieval.

Application

Application	Dilution info
IHC	1/100 - 1/200

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0809596	Certificate of Analysis	Aug 14, 2024	Ab116092
ZJ24F0809595	Certificate of Analysis	Aug 14, 2024	Ab116092

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Recombinant MSH2 Antibody - Primary antibody, specific to MSH2, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

Recombinant MSH2 Antibody - Primary antibody, specific to MSH2, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews