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Recombinant HIF1 beta Antibody - Primary antibody, specific to ARNT, Rabbit IgG

    Application:
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Hamster,Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab107495
Grouped product items
SKU Size
Availability
Price Qty
Ab107495-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab107495-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab107495-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab107495-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human HIF1 beta Antibody; WB, IHC; Unconjugated

Basic Description

Product Name Recombinant HIF1 beta Antibody - Primary antibody, specific to ARNT, Rabbit IgG
Synonyms arnT antibody | ARNT protein antibody | ARNT_HUMAN antibody | Aryl hydrocarbon receptor nuclear translocator antibody | bHLHe2 antibody | Class E basic helix-loop-helix protein 2 antibody | Dioxin receptor antibody | Dioxin receptor nuclear translocator a
Specifications & Purity Recombinant, ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of
Host species Rabbit
Specificity ARNT
Immunogen A synthetic peptide derived from human HIF-1 beta (AA 450-789)
Positive Control WB: HeLa, MCF7, Hep G2, HEK293 and C6 cell lysates. IHC: Human lung cancer cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human HIF1 beta Antibody, Recombinant, could be used for WB, IHC and so on.

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ARNT Tbio Aryl hydrocarbon receptor nuclear translocator (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
ARNT Tbio Aryl hydrocarbon receptor nuclear translocator (25 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant HIF1 beta Antibody (Ab107495) - Western Blot
All lanes: Recombinant HIF1 beta Antibody (Ab107495) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 87 kDa
Observed band size: 91 kDa
Exposure time: 37.2 s


Recombinant HIF1 beta Antibody (Ab107495) - Western Blot
All lanes: Recombinant HIF1 beta Antibody (Ab107495) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 87 kDa
Observed band size: 91 kDa
Exposure time: 37.2 s

Recombinant HIF1 beta Antibody (Ab107495) - IHC
Immunohistochemical analysis of HIF1 beta staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant HIF1 beta Antibody (Ab107495) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Application

Application Dilution info
WB

1/500-1/1000

IHC

1/50 - 1/100

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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