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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab107495-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
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Ab107495-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
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Ab107495-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$309.90
|
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Ab107495-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
|
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Recombinant; Rabbit anti Human HIF1 beta Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant HIF1 beta Antibody - Primary antibody, specific to ARNT, Rabbit IgG |
|---|---|
| Synonyms | arnT antibody | ARNT protein antibody | ARNT_HUMAN antibody | Aryl hydrocarbon receptor nuclear translocator antibody | bHLHe2 antibody | Class E basic helix-loop-helix protein 2 antibody | Dioxin receptor antibody | Dioxin receptor nuclear translocator a |
| Specifications & Purity | Recombinant, ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of |
| Host species | Rabbit |
| Specificity | ARNT |
| Immunogen | A synthetic peptide derived from human HIF-1 beta (AA 450-789) |
| Positive Control | WB: HeLa, MCF7, Hep G2, HEK293 and C6 cell lysates. IHC: Human lung cancer cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human HIF1 beta Antibody, Recombinant, could be used for WB, IHC and so on.
|
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Recombinant HIF1 beta Antibody (Ab107495) - Western Blot
All lanes: Recombinant HIF1 beta Antibody (Ab107495) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 91 kDa
Exposure time: 37.2 s
Recombinant HIF1 beta Antibody (Ab107495) - Western Blot
All lanes: Recombinant HIF1 beta Antibody (Ab107495) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 91 kDa
Exposure time: 37.2 s
Recombinant HIF1 beta Antibody (Ab107495) - IHC
Immunohistochemical analysis of HIF1 beta staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant HIF1 beta Antibody (Ab107495) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |