Grouped product items

SKU

Size

Availability

Price

Qty

Ab100025-10μl

10μl

$69.90

Ab100025-50μl

50μl

$199.90

Ab100025-100μl

100μl

Available within 8-12 weeks(?)

$309.90

Ab100025-1ml

1ml

Available within 8-12 weeks(?)

$2,399.90

Basic Description

Product Name	Recombinant DMAP1 Antibody - Primary antibody, specific to DMAP1, Rabbit IgG
Synonyms	DMAP 1 antibody \| DMAP1 antibody \| DMAP1_HUMAN antibody \| DNA methyltransferase 1 associated protein 1 antibody \| DNA methyltransferase 1-associated protein 1 antibody \| DNMAP 1 antibody \| DNMAP1 antibody \| DNMT1 associated protein 1 antibody \| DKFZp686L0
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.5 mg/mL
Host species	Rabbit
Specificity	DMAP1
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within human DMAP1.
Positive Control	WB: HEK293, HeLa, THP-1, MCF7, Hep G2, Jurkat, NIH/3T3 and C6 cell lysates. ICC/IF: MCF7 cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human DMAP1 Antibody, Recombinant, could be used for WB, ICC/IF and so on. Application WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Immunogen affinity purified
Shape	Liquid
Concentration	0.5 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

DMAP1 Tbio DNA methyltransferase 1-associated protein 1 (0 Activities)

Discover Target: DMAP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: Recombinant DMAP1 Antibody (Ab100025)

Recombinant DMAP1 Antibody (Ab100025) - Western Blot
All lanes: Recombinant DMAP1 Antibody (Ab100025) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 58 kDa
Exposure time: 120.0 s

IF/ICC: Recombinant DMAP1 Antibody (Ab100025)

Recombinant DMAP1 Antibody (Ab100025) - IF/ICC
IImmunofluorescent analysis of Recombinant DMAP1 Antibody (Ab100025) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5 - 10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin -AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Application

Application	Dilution info
IF/ICC	1/50-1/200
WB	1/500-1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

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Recombinant DMAP1 Antibody - Primary antibody, specific to DMAP1, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

Recombinant DMAP1 Antibody - Primary antibody, specific to DMAP1, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews