Grouped product items

SKU

Size

Availability

Price

Qty

Ab094903-10μl

10μl

$59.90

Ab094903-50μl

50μl

$159.90

Ab094903-100μl

100μl

Available within 8-12 weeks(?)

$259.90

Ab094903-1ml

1ml

Available within 8-12 weeks(?)

$2,399.90

Basic Description

Product Name

Recombinant CD45 Antibody - Primary antibody, specific to PTPRC, Rabbit IgG

Synonyms

Specifications & Purity

Recombinant, ExactAb™, Validated, See COA

Host species

Rabbit

Specificity

PTPRC

Immunogen

A synthetic peptide derived from human CD45 (AA 1250-1306)

Positive Control

WB: Jurkat and Raji cell lysates. IHC: Human spleen, Mouse skin, Mouse lung, Human placenta, Rat stomach tissue.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Product Description

Rabbit anti Human CD45 Antibody, Recombinant, could be used for WB, IHC and so on.

Application:

WB: 1/1000-1/2000
IHC: 1/100-1/200

Protein Function:

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Western Blot: Recombinant CD45 Antibody (Ab094903)

Recombinant CD45 Antibody (Ab094903) - Western Blot
All lanes: Recombinant CD45 Antibody (Ab094903) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 147 kDa
Observed band size: 231 kDa
Exposure time: 100.0 s

Western Blot: Recombinant CD45 Antibody (Ab094903)

Recombinant CD45 Antibody (Ab094903) - Western Blot
All lanes: Recombinant CD45 Antibody (Ab094903) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 147 kDa
Observed band size: 250 kDa
Exposure time: 100.0 s