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Recombinant CD146 Antibody - Primary antibody, specific to MCAM, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab093906
Grouped product items
SKU Size
Availability
 Price Qty
Ab093906-10μl
10μl
2
$69.90
Ab093906-50μl
50μl
1
$219.90
Ab093906-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$369.90
Ab093906-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human CD146 Antibody; WB, IHC, IF, ICC; Unconjugated

View related series
Click to view target information: MCAM
Accession#:P43121 (4) CD146 (4) Gene ID:4162 (4) MCAM (5) MUC18 (4)

Basic Description

Product Name Recombinant CD146 Antibody - Primary antibody, specific to MCAM, Rabbit IgG
Synonyms CD146 antigen | CD146 | cell surface glycoprotein MUC18 | Cell surface glycoprotein P1H12 | L-Gicerin | MCAM | melanoma adhesion molecule | melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen | Melanoma-associated antigen A32 | Melanoma
Specifications & Purity ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species Rabbit
Specificity MCAM
Immunogen A synthetic peptide derived from human CD146 (AA 628-640)
Positive Control WB: C6, HeLa lysates. IHC: Human colon cancer tissue. IF/ICC: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human CD146 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/50-1/200
Protein Function:
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Antigen Affinity Purified
Shape Liquid
Concentration 0.2 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MCAM Tbio Cell surface glycoprotein MUC18 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant CD146 Antibody (Ab093906) - Western Blot
All lanes: Recombinant CD146 Antibody (Ab093906) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 72 kDa
Observed band size: 111 kDa
Exposure time: 8.0 s

Recombinant CD146 Antibody (Ab093906) - IF/ICC
Immunocytochemistry analysis of CD146 (green) in HeLa using Recombinant CD146 Antibody (Ab093906), and cells were counterstained with DAPI (blue).

Recombinant CD146 Antibody (Ab093906) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using Recombinant CD146 Antibody (Ab093906). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000
IHC

1/50 - 1/100
IF/ICC

1/50 - 1/200

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0506017 Certificate of Analysis May 23, 2024 Ab093906
ZJ24F0506018 Certificate of Analysis May 23, 2024 Ab093906

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