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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab090212-10μl
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10μl |
1
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$79.90
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Ab090212-50μl
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50μl |
1
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$219.90
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Ab090212-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$349.90
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Ab090212-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
|
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Recombinant; Rabbit anti Human ATPB Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | Recombinant ATPB Antibody - Primary antibody, specific to ATP5F1B, Rabbit IgG |
|---|---|
| Synonyms | ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody | ATP synthase subunit beta mitochondrial antibody | ATP synthase subunit beta, mitochondrial antibody | atp5b antibody | ATPB antibody | ATPB_HUMAN antibody | ATPMB antibody |
| Specifications & Purity | Recombinant, ExactAb™, Validated, High Performance, See COA |
| Biochemical and Physiological Mechanisms | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of |
| Host species | Rabbit |
| Specificity | ATP5F1B |
| Immunogen | A synthetic peptide derived from human ATPB (AA 151-164) |
| Positive Control | WB: Jurkat, HeLa, Raji, Hep G2, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description |
Rabbit anti Human ATPB Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
|
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Recombinant ATPB Antibody (Ab090212) - Western Blot
All lanes: Recombinant ATPB Antibody (Ab090212) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 52 kDa
Exposure time: 1.9 s
Recombinant ATPB Antibody (Ab090212) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant ATPB Antibody (Ab090212). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant ATPB Antibody (Ab090212) - IF/ICC
Immunocytochemistry analysis of ATPB (green) in HeLa cells using Recombinant ATPB Antibody (Ab090212) and DAPI (blue).
| Application | Dilution info |
|---|---|
| IHC | 1/100-1/200 |
| IF/ICC | 1/200 - 1/500 |
| WB | 1/2000-1/20000 |