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Recombinant Alkaline Phosphatase Antibody - Primary antibody, specific to Alkaline Phosphatase, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab088188
Grouped product items
SKU Size
Availability
 Price Qty
Ab088188-10μl
10μl
1
$69.90
Ab088188-50μl
50μl
1
$199.90
Ab088188-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab088188-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,099.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human Alkaline Phosphatase Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Click to view target information: ALPL
Accession#:P05186 (3) Alkaline Phosphatase (8) ALPL (3) Gene ID:249 (3)

Basic Description

Product Name Recombinant Alkaline Phosphatase Antibody - Primary antibody, specific to Alkaline Phosphatase, Rabbit IgG
Synonyms Alkaline phosphatase, germ cell antibody | Alkaline phosphatase, testicular and thymus antibody | Akp2 antibody | Alkaline phosphatase intestinal antibody | Alkaline phosphatase intestinal precursorantibody | Alkaline phosphatase liver/bone/kidney antibod
Specifications & Purity Recombinant, ExactAb™, Validated, High Performance, See COA
Host species Rabbit
Specificity Alkaline Phosphatase
Immunogen A synthetic peptide derived from human Alkaline Phosphatase (AA 1-61).
Positive Control WB: Mouse kidney, Rat kidney, HeLa, Hep G2 and JAR lysates. IHC: Human liver tissue. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, High Performance, Recombinant, Validated
Product Description

Rabbit anti Human Alkaline Phosphatase Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/5000-1/10000
IHC: 1/50-1/200
ICC/IF: 1/50-1/100
Protein Function：
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ALPL Tchem Alkaline phosphatase, tissue-nonspecific isozyme (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant Alkaline Phosphatase Antibody (Ab088188) - Western Blot
All lanes: Recombinant Alkaline Phosphatase Antibody (Ab088188) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 75 kDa
Exposure time: 60.0 s

Recombinant Alkaline Phosphatase Antibody (Ab088188) - Western Blot
All lanes: Recombinant Alkaline Phosphatase Antibody (Ab088188) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 75 kDa
Exposure time: 30.9 s


Recombinant Alkaline Phosphatase Antibody (Ab088188) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Alkaline Phosphatase Antibody (Ab088188). 

Recombinant Alkaline Phosphatase Antibody (Ab088188) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Alkaline Phosphatase Antibody (Ab088188) at 1/50 dilution.

Recombinant Alkaline Phosphatase Antibody (Ab088188) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Alkaline Phosphatase Antibody (Ab088188) at 1/150 dilution. 

Recombinant Alkaline Phosphatase Antibody (Ab088188) - IHC
Immunohistochemical analysis of paraffin-embedded human liver, using Recombinant Alkaline Phosphatase Antibody (Ab088188).

Application

Application Dilution info
WB

1/5000 – 1/10000

IHC

1/50-1/200
IF/ICC

1/50-1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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