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AKAP 95 Antibody - Primary antibody, specific to AKAP8, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab087835
Grouped product items
SKU Size
Availability
Price Qty
Ab087835-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$79.90
Ab087835-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$229.90
Ab087835-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$379.90
Ab087835-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,199.90

pAb; Rabbit anti Human AKAP 95 Antibody; WB, IHC, IF/ICC; Unconjugated

Basic Description

Product Name AKAP 95 Antibody - Primary antibody, specific to AKAP8, Rabbit IgG
Synonyms A kinase (PRKA) anchor protein 8 antibody | A kinase anchor protein 8 antibody | A kinase anchor protein 95 antibody | A kinase anchor protein 95 kDa antibody | A kinase anchor protein 95kDa antibody | A-kinase anchor protein 8 antibody | A-kinase anchor
Specifications & Purity ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Biochemical and Physiological Mechanisms Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).
Host species Rabbit
Specificity AKAP8
Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AKAP 95.
Positive Control WB: Hep G2, Jurkat and HEK293 cell lysates. IHC: Human heart tissue. IF/ICC: Jurkat cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human AKAP 95 Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.

Product Properties

Isotype Rabbit IgG
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

AKAP8 Tbio A-kinase anchor protein 8 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

AKAP 95 Antibody (Ab087835) - Western Blot
All lanes: AKAP 95 Antibody (Ab087835) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 76 kDa
Observed band size: 91 kDa
Exposure time: 60.0 s

AKAP 95 Antibody (Ab087835) - Western Blot
All lanes: AKAP 95 Antibody (Ab087835) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 76 kDa
Observed band size: 91 kDa
Exposure time: 60.0 s

AKAP 95 Antibody (Ab087835) - Western Blot
Immunohistochemical analysis of AKAP8 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with AKAP 95 Antibody (Ab087835) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

AKAP 95 Antibody (Ab087835) - IF/ICC
Immunofluorescent analysis of AKAP8 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with AKAP 95 Antibody (Ab087835) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Application

Application Dilution info
WB

1/500-1/1000

IHC

1/50 - 1/100

IF/ICC

1/50 - 1/200

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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