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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab087835-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$79.90
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Ab087835-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$229.90
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Ab087835-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$379.90
|
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Ab087835-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,199.90
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pAb; Rabbit anti Human AKAP 95 Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | AKAP 95 Antibody - Primary antibody, specific to AKAP8, Rabbit IgG |
|---|---|
| Synonyms | A kinase (PRKA) anchor protein 8 antibody | A kinase anchor protein 8 antibody | A kinase anchor protein 95 antibody | A kinase anchor protein 95 kDa antibody | A kinase anchor protein 95kDa antibody | A-kinase anchor protein 8 antibody | A-kinase anchor |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). |
| Host species | Rabbit |
| Specificity | AKAP8 |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AKAP 95. |
| Positive Control | WB: Hep G2, Jurkat and HEK293 cell lysates. IHC: Human heart tissue. IF/ICC: Jurkat cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human AKAP 95 Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.
|
| Isotype | Rabbit IgG |
|---|---|
| Purification Method | Immunogen affinity purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
AKAP 95 Antibody (Ab087835) - Western Blot
All lanes: AKAP 95 Antibody (Ab087835) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 76 kDa
Observed band size: 91 kDa
Exposure time: 60.0 s
AKAP 95 Antibody (Ab087835) - Western Blot
All lanes: AKAP 95 Antibody (Ab087835) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 76 kDa
Observed band size: 91 kDa
Exposure time: 60.0 s
AKAP 95 Antibody (Ab087835) - Western Blot
Immunohistochemical analysis of AKAP8 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with AKAP 95 Antibody (Ab087835) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
AKAP 95 Antibody (Ab087835) - IF/ICC
Immunofluorescent analysis of AKAP8 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with AKAP 95 Antibody (Ab087835) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
| IF/ICC | 1/50 - 1/200 |