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PSMD9 Antibody - Primary antibody, specific to PSMD9, Rabbit IgG

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PSMD9 Antibody - Primary antibody, specific to PSMD9, Rabbit IgG

Grade & Purity:

See COA

Application:

Flow Cytometry
IHC
WB

Host species: Rabbit
Reactive species: Human,Mouse,Rat
Isotype: Rabbit IgG
Conjugation: Unconjugated

In stock

Item Number

Ab123714

Grouped product items
SKU	Size	Availability	Price	Qty
Ab123714-10μl	10μl	6	$69.90
Ab123714-50μl	50μl	4	$189.90
Ab123714-100μl	100μl	2	$249.90
Ab123714-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,999.90

pAb; Rabbit anti Human PSMD9 Antibody; WB, IHC, Flow; Unconjugated

View related series

Click to view target information: PSMD9

26S proteasome subunit 9 (1) Accession#: O00233 (1) Flow Cytometry (1158) Gene ID: 5715 (1) PSMD9 (1)

Basic Description

Product Name	PSMD9 Antibody - Primary antibody, specific to PSMD9, Rabbit IgG
Synonyms	26S proteasome non-ATPase regulatory subunit 9 antibody \| 26S proteasome regulatory subunit p27 antibody \| Homolog of rat Bridge 1 antibody \| MGC8644 antibody \| p27 antibody \| Proteasome (prosome macropain) 26S subunit non ATPase 9 antibody \| Proteasome 2
Specifications & Purity	Carrier Free, KO Validation, ExactAb™, Azide Free, Validated, See COA
Host species	Rabbit
Specificity	PSMD9
Immunogen	Recombinant protein of human PSMD9 (AA 2-223).
Positive Control	WB: A549, HeLa, Hep G2, Jurkat, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human liver, liver cancer, glioma, lung cancer, breast cancer, prostate tissues. Flow: Human SiHa cells.
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, KO Validation, Validated
Product Description	Rabbit anti Human PSMD9 Antibody, Polyclonal, could be used for WB, IHC, Flow and so on. Application: WB: 0.01-5 µg/mL IHC: 5-20 µg/mL Flow: 5-20µg/mL per 10^6 cells in 100 µl volume Protein Function: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified + Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

PSMD9 Tbio 26S proteasome non-ATPase regulatory subunit 9 (0 Activities)

Discover Target: PSMD9

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: PSMD9 Antibody (Ab123714)

PSMD9 Antibody (Ab123714) - Western Blot
All lanes: PSMD9 Antibody (Ab123714) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 23, 28 kDa
Exposure time: 23.7 s

IHC: PSMD9 Antibody (Ab123714)

PSMD9 Antibody (Ab123714) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PSMD9 Antibody (Ab123714)

PSMD9 Antibody (Ab123714) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PSMD9 Antibody (Ab123714)

PSMD9 Antibody (Ab123714) - IHC
DAB staining on IHC
Samples: Human Glioma Tissue
Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PSMD9 Antibody (Ab123714)

PSMD9 Antibody (Ab123714) - IHC
DAB staining on IHC
Samples: Human Lung cancer Tissue
Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PSMD9 Antibody (Ab123714)

PSMD9 Antibody (Ab123714) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PSMD9 Antibody (Ab123714)

PSMD9 Antibody (Ab123714) - IHC
DAB staining on IHC
Samples: Human Prostate Tissue
Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Flow Cytometry: PSMD9 Antibody (Ab123714)

PSMD9 Antibody (Ab123714) - Flow Cytometry
Human SiHa cell was fixed with 2% paraformaldehyde (10 min) ,
permeabilised with 0.1% BSA-Triton X-100, then stained with 20 µg/mL PSMD9 Antibody (Ab123714) (red histogram) or Isotype control antibody (green histogram), followed by 1 µg/mL FITC-conjugated Anti-rabbit IgG Secondary Antibody.

Application

Application	Dilution info
IHC	5-20 µg/mL
WB	0.01-5 µg/mL
Flow Cytometry	5-20µg/mL per 10^6 cells in 100 µl volume

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0707901	Certificate of Analysis	Jul 08, 2024	Ab123714
ZJ24F0707902	Certificate of Analysis	Jul 08, 2024	Ab123714
ZJ24F0707900	Certificate of Analysis	Jul 08, 2024	Ab123714

Documents & Articles

Flow Cytometry Using Methanol Permeabilization

Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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