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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab122866-10μl
|
10μl |
1
|
$59.90
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Ab122866-50μl
|
50μl |
1
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$169.90
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Ab122866-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$229.90
|
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Ab122866-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
|
|
pAb; Rabbit anti Human PPP2R5A Antibody; WB, ICC, IF; Unconjugated
| Product Name | PPP2R5A Antibody - Primary antibody, specific to PPP2R5A, Rabbit IgG |
|---|---|
| Synonyms | B56A antibody | 2A5A_HUMAN antibody | MGC131915 antibody | PP2A B subunit B' alpha isoform antibody | PP2A B subunit B56 alpha isoform antibody | PP2A B subunit isoform B''-alpha antibody | PP2A B subunit isoform B56-alpha antibody | PP2A B subunit isofor |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | PPP2R5A |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PPP2R5A. |
| Positive Control | WB: Mouse skeletal muscle, Jurkat and NIH/3T3 lysates. IF/ICC: NIH/3T3 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human PPP2R5A Antibody, Polyclonal, could be used for WB, ICC/IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
PPP2R5A Antibody (Ab122866) - Western Blot
All lanes: PPP2R5A Antibody (Ab122866) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 56 kDa
Observed band size: 52 kDa
Exposure time: 266.0 s
PPP2R5A Antibody (Ab122866) - IF/ICC
Immunofluorescent analysis of PPP2R5A staining in NIH/3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with PPP2R5A Antibody (Ab122866) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
| Application | Dilution info |
|---|---|
| WB |
1/500-1/1000
|
| ICC/IF | 1/100-1/500 |