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NECAB3 Antibody - Primary antibody, specific to NECAB3, Rabbit IgG

Grade & Purity:

1.0 mg/mL

Application:

IF/ICC
IHC
WB

Host species: Rabbit
Reactive species: Cow,Human,Mouse,Rat
Isotype: Rabbit IgG
Conjugation: Unconjugated

In stock

Item Number

Ab191500

Grouped product items
SKU	Size	Availability	Price
Ab191500-10μl	10μl	1	$69.90
Ab191500-50μl	50μl	1	$209.90
Ab191500-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$309.90
Ab191500-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,399.90

pAb; Rabbit anti Human NECAB3 Antibody; WB, IHC, ICC, IF; Unconjugated

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Click to view target information: NECAB3

Accession#: Q96P71 (1) Gene ID: 63941 (1) NECAB3 (1)

Basic Description

Product Name	NECAB3 Antibody - Primary antibody, specific to NECAB3, Rabbit IgG
Synonyms	APBA2BP \| NIP1 \| SYTIP2 \| XB51 \| N-terminal EF-hand calcium-binding protein 3 \| Amyloid beta A4 protein-binding family A member 2-binding protein \| Nek2-interacting protein 1 \| Neuronal calcium-binding protein 3 \| X11L-binding protein 51 \| NECAB3 \| STIP3
Specifications & Purity	ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Biochemical and Physiological Mechanisms	Inhibits the interaction of APBA2 with amyloid-beta precursor protein (APP), and hence allows formation of amyloid-beta. May enhance the activity of HIF1A and thus promote glycolysis under normoxic conditions; the function requires its ABM domain and may
Host species	Rabbit
Specificity	NECAB3
Immunogen	A KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NIP1.
Positive Control	WB: COLO 205, HT-29 and Hep G2 cell lysates. IHC: Human lung cancer tissue. IF/ICC: COLO 205 cells.
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, Validated
Product Description	Rabbit anti Human NECAB3 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Immunogen affinity purified
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

NECAB3 Tbio N-terminal EF-hand calcium-binding protein 3 (0 Activities)

Discover Target: NECAB3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: NECAB3 Antibody (Ab191500)

NECAB3 Antibody (Ab191500) - Western Blot
All lanes: NECAB3 Antibody (Ab191500) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 100.0 s

IHC: NECAB3 Antibody (Ab191500)

NECAB3 Antibody (Ab191500) - IHC
Immunohistochemical analysis of NECAB3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with NECAB3 Antibody (Ab191500) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: NECAB3 Antibody (Ab191500)

NECAB3 Antibody (Ab191500) - IF/ICC
Immunofluorescent analysis of NECAB3 staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the NECAB3 Antibody (Ab191500) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Application

Application	Dilution info
WB	1/500-1/1000
IF/ICC	1/50-1/200
IHC	1/50 - 1/100

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NECAB3 Antibody - Primary antibody, specific to NECAB3, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

NECAB3 Antibody - Primary antibody, specific to NECAB3, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews