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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab116570-10μl
|
10μl |
1
|
$69.90
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Ab116570-50μl
|
50μl |
1
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$199.90
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Ab116570-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$319.90
|
|
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Ab116570-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
|
|
pAb; Rabbit anti Human MVK Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | MVK Antibody - Primary antibody, specific to MVK, Rabbit IgG |
|---|---|
| Synonyms | LH receptor mRNA binding protein | LRBP | Mevalonate kinase | Mevalonic aciduria | MK antibody MVLK | KIME_HUMAN | FLJ96772 antibody | KIME_HUMAN antibody | LH receptor mRNA binding protein antibody | LRBP antibody | Mevalonate kinase 1 antibody | Mevalon |
| Specifications & Purity | ExactAb™, Carrier Free, Validated, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | May be a regulatory site in cholesterol biosynthetic pathway. |
| Host species | Rabbit |
| Specificity | MVK |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MVK |
| Positive Control | WB: Jurkat, A431, MCF7 and Hep G2 cell lysates. IHC: Human breast cancer tissue. IF/ICC: Hep G2 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human MVK Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
MVK Antibody (Ab116570) - Western Blot
All lanes: MVK Antibody (Ab116570) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 38.6 s
MVK Antibody (Ab116570) - Western Blot
All lanes: MVK Antibody (Ab116570) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 38.6 s
MVK Antibody (Ab116570) - IF/ICC
Immunofluorescent analysis of MVK staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with MVK Antibody (Ab116570) in 3% BSA-PBS and incubated overnight at 4 °C in a humidity chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MVK Antibody (Ab116570) - IHC
Immunohistochemical analysis of MVK staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with MVK Antibody (Ab116570) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
| Application | Dilution info |
|---|---|
| IHC | 1/100 - 1/200 |
| IF/ICC |
1/100-1/500
|
| WB |
1/500-1/1000
|