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KAP1 Mouse mAb - Primary antibody, specific to KAP1, IgG1

COA COA
    Application:
  • IF/ICC
  • IHC
  • IHC-F
  • IHC-P
  • IP
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2b
  • Clone Number: 4E1-D12-F8
  • Conjugation: Unconjugated
In stock
Item Number
Ab111687
Grouped product items
SKU Size
Availability
 Price Qty
Ab111687-10μl
10μl
5
$69.90
Ab111687-50μl
50μl
2
$207.90
Ab111687-100μl
100μl
1
$297.90
Ab111687-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,097.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb(4E1-D12-F8); Mouse anti Human KAP1 Antibody; WB, IHC-F, IHC-P, ICC/IF, IP; Unconjugated

View related series
Click to view target information: TRIM28
Accession#:Q13263 (1) Gene ID:10155 (1) KAP1 (1) TRIM28 (1)

Basic Description

Product Name KAP1 Mouse mAb - Primary antibody, specific to KAP1, IgG1
Synonyms E3 SUMO protein ligase TRIM28 antibody | E3 SUMO-protein ligase TRIM28 antibody | FLJ29029 antibody | KAP 1 antibody | KAP-1 antibody | KRAB associated protein 1 antibody | KRAB interacting protein 1 antibody | KRAB-associated protein 1 antibody | KRAB-in
Specifications & Purity ExactAb™, Validated, 1.0mg/mL
Host species Mouse
Specificity KAP1
Immunogen Purified recombinant human KAP1/TIF1 beta protein fragments expressed in E.coli (AA 548-825).
Positive Control WB: A549, Hep G2, HEK293, MCF7, HeLa, A431 lysates. IP: HeLa lysates. ICC/IF: HeLa cells. IHC: Human Spleen Tissue .
Conjugation Unconjugated
Grade ExactAb™, Validated
Product Description

Mouse anti Human KAP1 Antibody, 4E1-D12-F8, could be used for WB, IHC-F, IHC-P, ICC/IF, IP and so on.

Application:
WB: 1/500-1/1000
IHC-F: 1/50-1/100
IHC-P: 1/50-1/100
ICC/IF: 1/50-1/200
IP: 1/20

Protein Function
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.

Post-translational
Phosphorylated upon DNA damage, probably by ATM or ATR. ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes. Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21.

Product Properties

Isotype IgG1
Light Chain Type kappa
SDS-PAGE 150kDa
Purification Method Protein G purified
Shape Liquid
Concentration 1.0mg/mL
Storage Temp Store at -20°C
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

TRIM28 Tbio Transcription intermediary factor 1-beta (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

KAP1 Mouse mAb (Ab111687) - Western Blot
All lanes: KAP1 Mouse mAb (Ab111687) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 100&75 kDa
Observed band size: 100&72 kDa

KAP1 Mouse mAb (Ab111687) - IP
Immunoprecipitation analysis of KAP1 in HeLa lysates using KAP1 Mouse mAb (Ab111687).

KAP1 Mouse mAb (Ab111687) - IF
IF analysis of KAP1 in HeLa using KAP1 Mouse mAb (Ab111687) .

KAP1 Mouse mAb (Ab111687) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Spleen using KAP1 Mouse mAb (Ab111687). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000
IF/ICC

1/50-1/200

IP

1/20
IHC-P

1/50-1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0700725 Certificate of Analysis Jul 31, 2023 Ab111687
ZJ23F0700724 Certificate of Analysis Jul 31, 2023 Ab111687

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