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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab106204-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$79.90
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Ab106204-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$229.90
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Ab106204-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$379.90
|
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Ab106204-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
|
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pAb; Rabbit anti Human GPI-PLD Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | GPI-PLD Antibody - Primary antibody, specific to GPI-PLD, Rabbit IgG |
|---|---|
| Synonyms | Glycosyl-phosphatidylinositol-specific phospholipase D antibody | Glycoprotein phospholipase D antibody | GPI-PLD antibody | GPI-specific phospholipase D antibody | GPIPLD antibody | GPIPLDM antibody | GPLD1 antibody | MGC22590 antibody | PHLD_HUMAN antib |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane. |
| Host species | Rabbit |
| Specificity | GPI-PLD |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPIPLD. |
| Positive Control | WB: Mouse brian lysates. IHC: Human kidney cancer tissue. IF/ICC: MCF7 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human GPI-PLD Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.
|
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
GPI-PLD Antibody (Ab106204) - Western Blot
All lanes: GPI-PLD Antibody (Ab106204) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 121 kDa
Exposure time: 20.0 s
GPI-PLD Antibody (Ab106204) - IF/ICC
Immunofluorescent analysis of GPIPLD staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GPI-PLD Antibody (Ab106204) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).
GPI-PLD Antibody (Ab106204) - IHC
Immunohistochemical analysis of GPIPLD staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with GPI-PLD Antibody (Ab106204) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| IF/ICC |
1/50-1/200
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| IHC | 1/50-1/100
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