This is a demo store. No orders will be fulfilled.

DNAJC3 Antibody - Primary antibody, specific to DNAJC3, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Cow,Human,Mouse,Pig,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab100175
Grouped product items
SKU Size
Availability
Price Qty
Ab100175-10μl
10μl
1
$49.90
Ab100175-50μl
50μl
1
$139.90
Ab100175-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$229.90
Ab100175-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

pAb; Rabbit anti Human DNAJC3 Antibody; WB, IHC, ICC, IF; Unconjugated

Basic Description

Product Name DNAJC3 Antibody - Primary antibody, specific to DNAJC3, Rabbit IgG
Synonyms DnaJ (Hsp40) homolog subfamily C member 3 antibody | DnaJ homolog subfamily C member 3 antibody | DNAJC3 antibody | DNJC3_HUMAN antibody | double-stranded RNA-activated protein kinase inhibitor antibody | Endoplasmic reticulum DnaJ protein 6 antibody | ER
Specifications & Purity ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Biochemical and Physiological Mechanisms Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein
Host species Rabbit
Specificity DNAJC3
Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DnaJC3.
Positive Control WB: COLO 205 and Raji cell lysates. IHC: Human colorectal cancer tissue. IF/ICC: SGC7901 cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human DNAJC3 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

DNAJC3 Tbio DnaJ homolog subfamily C member 3 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

DNAJC3 Antibody (Ab100175) - Western Blot
All lanes: DNAJC3 Antibody (Ab100175) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 57 kDa
Exposure time: 3.7 s

DNAJC3 Antibody (Ab100175) - Western Blot
All lanes: DNAJC3 Antibody (Ab100175) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 57 kDa
Exposure time: 3.7 s


DNAJC3 Antibody (Ab100175) - IHC
Immunohistochemical analysis of DNAJC3 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with DNAJC3 Antibody (Ab100175) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

DNAJC3 Antibody (Ab100175) - IF/ICC
Immunofluorescent analysis of DNAJC3 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with DNAJC3 Antibody (Ab100175) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Application

Application Dilution info
WB

1/500-1/2000

IHC

1/50 - 1/100

IF/ICC

1/50 - 1/200

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

Shall we send you a message when we have discounts available?

Remind me later

Thank you! Please check your email inbox to confirm.

Oops! Notifications are disabled.