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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab100175-10μl
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10μl |
1
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$49.90
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Ab100175-50μl
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50μl |
1
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$139.90
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Ab100175-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$229.90
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Ab100175-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$1,999.90
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pAb; Rabbit anti Human DNAJC3 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | DNAJC3 Antibody - Primary antibody, specific to DNAJC3, Rabbit IgG |
|---|---|
| Synonyms | DnaJ (Hsp40) homolog subfamily C member 3 antibody | DnaJ homolog subfamily C member 3 antibody | DNAJC3 antibody | DNJC3_HUMAN antibody | double-stranded RNA-activated protein kinase inhibitor antibody | Endoplasmic reticulum DnaJ protein 6 antibody | ER |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein |
| Host species | Rabbit |
| Specificity | DNAJC3 |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DnaJC3. |
| Positive Control | WB: COLO 205 and Raji cell lysates. IHC: Human colorectal cancer tissue. IF/ICC: SGC7901 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human DNAJC3 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
DNAJC3 Antibody (Ab100175) - Western Blot
All lanes: DNAJC3 Antibody (Ab100175) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 57 kDa
Exposure time: 3.7 s
DNAJC3 Antibody (Ab100175) - Western Blot
All lanes: DNAJC3 Antibody (Ab100175) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 57 kDa
Exposure time: 3.7 s
DNAJC3 Antibody (Ab100175) - IHC
Immunohistochemical analysis of DNAJC3 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with DNAJC3 Antibody (Ab100175) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
DNAJC3 Antibody (Ab100175) - IF/ICC
Immunofluorescent analysis of DNAJC3 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with DNAJC3 Antibody (Ab100175) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| IHC | 1/50 - 1/100 |
| IF/ICC | 1/50 - 1/200 |