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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab098684-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$69.90
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Ab098684-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
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Ab098684-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$319.90
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Ab098684-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,499.90
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pAb; Rabbit anti Human Cytochrome b5 Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | Cytochrome b5 Antibody - Primary antibody, specific to Cytochrome b5, Rabbit IgG |
|---|---|
| Synonyms | CYB5A | CYB5 | MCB5 | METAG | CYB 5 antibody | CYB 5A antibody | CYB5 antibody | CYB5_HUMAN antibody | Cyb5a antibody | Cytochrome b 5 antibody | Cytochrome b5 (microsomal) antibody | Cytochrome b5 antibody | Cytochrome b5 type A (microsomal) antibody | C |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. |
| Host species | Rabbit |
| Specificity | Cytochrome b5 |
| Immunogen | A synthetic peptide derived from human Cytochrome b5A |
| Positive Control | WB: HepG2 lysates. IHC: Human liver cancer tissue. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human Cytochrome b5 Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Cytochrome b5 Antibody (Ab098684) - Western Blot
All lanes: Cytochrome b5 Antibody (Ab098684) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 100.0 s
Cytochrome b5 Antibody (Ab098684) - IHC
Immunohistochemical analysis of Cytochrome b5A staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Cytochrome b5 Antibody (Ab098684) at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Cytochrome b5 Antibody (Ab098684) - IF/ICC
Immunofluorescent analysis of Cytochrome b5A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Cytochrome b5 Antibody (Ab098684) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
| Application | Dilution info |
|---|---|
| IHC | 1/100 - 1/200 |
| WB | 1/500-1/1000 |
| IF/ICC |
1/100-1/500
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