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CD3 Mouse mAb (AF405) - Primary antibody, specific to CD3

    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: UCHT1
  • Conjugation: AF405
In stock
Item Number
Ab189660
Grouped product items
SKU Size
Availability
Price Qty
Ab189660-10T
10T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$49.90
Ab189660-25T
25T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$89.90
Ab189660-100T
100T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$249.90

mAb (UCHT1); Mouse anti Human CD3 Antibody (AF405); Flow Cytometry

Basic Description

Product Name CD3 Mouse mAb (AF405) - Primary antibody, specific to CD3
Synonyms CD3epsilon | IMD18 | CD3 epsilon | CD3e antigen | CD3e molecule | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex | T-cell surface antigen T3/Leu-4 epsilon chain | T-cell surface glycoprotein CD3 epsilon chain | TCRE | CD3 epsilon an
Specifications & Purity ExactAb™, Validated, Ex:401nm, Em:422nm, 5 μL/test
Biochemical and Physiological Mechanisms The CD3 complex mediates signal transduction.
Host species Mouse
Specificity CD3
Immunogen Human CD3ε
Positive Control Flow: Human peripheral blood lymphocytes
Conjugation AF405
Excitation(nm) Violet Laser(405nm)
Emission(nm) 422nm
Ex/Em(nm) Ex:401nm;Em:422nm
Grade Em:422nm, Ex:401nm, ExactAb™, Validated
Product Description

Mouse anti Human CD3 Antibody, Monoclonal (UCHT1), could be used for Flow.

Product Properties

Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Concentration 5 μL/test
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

CD3 Mouse mAb (AF405) (Ab189660) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD3 Mouse mAb (AF405) (Ab189660) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF405) (Ab190076).

CD3 Mouse mAb (AF405) (Ab189660) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD3 Mouse mAb (AF405) (Ab189660) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF405) (Ab190076). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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