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Recombinant CD3 Antibody, specific to Human CD3ε

    Application:
  • ELISA
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: P93B35
  • Conjugation: Unconjugated
In stock
Item Number
Ab219596
Grouped product items
SKU Size
Availability
Price Qty
Ab219596-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$10.90
Ab219596-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$29.90
Ab219596-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$41.90
Ab219596-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$139.90

Recombinant (P93B35); Mouse anti Human CD3ε Antibody; Flow Cytometry, ELISA; Unconjugated

Basic Description

Product Name Recombinant CD3 Antibody, specific to Human CD3ε
Specifications & Purity Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), See COA
Biochemical and Physiological Mechanisms Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane b
Host species Mouse
Specificity Human CD3ε
Immunogen Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
Positive Control Flow Cytometry: Human peripheral blood lymphocytes
Conjugation Unconjugated
Grade Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Recombinant, Validated
Product Description

Recombinant CD3ε Antibody, clone P93B35, could be used for Flow Cytometry, ELISA and so on.

Product Properties

Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C long time. Upon delivery aliquot. Avoid freeze / thaw cycle.

Associated Targets(Human)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant CD3 Antibody (Ab219596) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD3 with Recombinant CD3 Antibody (Ab219596) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Mouse IgG (Ab170221) - Isotype Control (left panel) and detected with Alexa Fluor 488-conjugated affinipure goat anti-Mouse IgG.

ecombinant CD3 Antibody (Ab219596) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD3 (red) with Recombinant CD3 Antibody (Ab219596) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with Alexa Fluor 488-conjugated affinipure goat anti-Mouse IgG. Blue - Isotype control, compared with Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Recombinant CD3 Antibody (Ab219596) - SEC
The purity of Recombinant CD3 Antibody (Ab219596) is more than 95% verified by HPLC.

Application

Application Dilution info
Flow Cytometry

≤0.5 µg per million cells in 100 µl volume

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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