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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab219596-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$10.90
|
|
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Ab219596-50μg
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50μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$29.90
|
|
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Ab219596-100μg
|
100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$41.90
|
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Ab219596-1mg
|
1mg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$139.90
|
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Recombinant (P93B35); Mouse anti Human CD3ε Antibody; Flow Cytometry, ELISA; Unconjugated
| Product Name | Recombinant CD3 Antibody, specific to Human CD3ε |
|---|---|
| Specifications & Purity | Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), See COA |
| Biochemical and Physiological Mechanisms | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane b |
| Host species | Mouse |
| Specificity | Human CD3ε |
| Immunogen | Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor |
| Positive Control | Flow Cytometry: Human peripheral blood lymphocytes |
| Conjugation | Unconjugated |
| Grade | Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Recombinant, Validated |
| Product Description |
Recombinant CD3ε Antibody, clone P93B35, could be used for Flow Cytometry, ELISA and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C long time. Upon delivery aliquot. Avoid freeze / thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Recombinant CD3 Antibody (Ab219596) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD3 with Recombinant CD3 Antibody (Ab219596) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Mouse IgG (Ab170221) - Isotype Control (left panel) and detected with Alexa Fluor 488-conjugated affinipure goat anti-Mouse IgG.
ecombinant CD3 Antibody (Ab219596) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD3 (red) with Recombinant CD3 Antibody (Ab219596) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with Alexa Fluor 488-conjugated affinipure goat anti-Mouse IgG. Blue - Isotype control, compared with Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
Recombinant CD3 Antibody (Ab219596) - SEC
The purity of Recombinant CD3 Antibody (Ab219596) is more than 95% verified by HPLC.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤0.5 µg per million cells in 100 µl volume |
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