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CD3 Mouse mAb (PE-Cy5) - Primary antibody, specific to CD3

    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: UCHT1
  • Conjugation: PE-Cy5
In stock
Item Number
Ab189665
Grouped product items
SKU Size
Availability
Price Qty
Ab189665-10T
10T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$49.90
Ab189665-25T
25T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$99.90
Ab189665-100T
100T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$245.90

mAb (UCHT1); Mouse anti Human CD3 Antibody (PE-Cy5); Flow Cytometry

Basic Description

Product Name CD3 Mouse mAb (PE-Cy5) - Primary antibody, specific to CD3
Synonyms CD3epsilon | IMD18 | CD3 epsilon | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, epsilon (CD3-TCR complex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex, epsilon subunit of T3 | T-cell surface a
Specifications & Purity ExactAb™, Validated, 5 μL/test
Biochemical and Physiological Mechanisms The CD3 complex mediates signal transduction.
Host species Mouse
Specificity CD3
Immunogen Human CD3ε
Positive Control Flow Cytometry: Human peripheral blood lymphocytes
Conjugation PE-Cy5
Excitation(nm) Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm) 670nm
Ex/Em(nm) Ex:565nm;Em:670nm
Grade ExactAb™, Validated
Product Description

Mouse anti Human CD3 Antibody, Monoclonal (UCHT1), could be used for Flow Cytometry

Product Properties

Light Chain Type kappa
Purification Method Protein G purified
Concentration 5 μL/test
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

CD3 Mouse mAb (PE-Cy5) (Ab189665) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD3 Mouse mAb (PE-Cy5) (Ab189665) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE-Cy5) (Ab190078).

CD3 Mouse mAb (PE-Cy5) (Ab189665) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD3 Mouse mAb (PE-Cy5) (Ab189665) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE-Cy5) (Ab190078). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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